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    • 3. 发明申请
    • FIBER-OPTIC SENSOR ARRAY
    • 光纤传感器阵列
    • WO03003063A3
    • 2003-04-17
    • PCT/US0220596
    • 2002-06-27
    • IA INCPRIUSKA ERIC MSMITH RICHARD HERB JUDITH LDOWNWARD JAMES GULRICH OTHO E
    • PRIUSKA ERIC MSMITH RICHARD HERB JUDITH LDOWNWARD JAMES GULRICH OTHO E
    • G01N21/25G01N21/64G01N33/533G01N33/543G01N33/53
    • G01N21/648G01N21/6428G01N21/6452G01N21/6458G01N33/533G01N33/54306G01N2021/6439G01N2021/6441G01N2021/6482
    • A method for performing a rapid, homogenous assays for monitoring the reactions of a binding target, by immobilizing a fluorescent-capable chelate complex that is derivatized so as to posses recognition binding ligands, labeling the complex with a labeled second chelator that is added to the assay thereby forming a fluorescent mixed chelate, and measuring the fluorescent mixed chelate, whereby the measurement of the label enable monitoring of the reaction of the binding target. A rapid assay for performing the above method including a first chelating molecule, a fluorescent-capable ion complexed with the first chelating molecule, a second chelating molecule for reacting with the fluorescent-capable ion complexed with the first chelating molecule, and a measuring device for measuring fluorescent resulting from the second chelating molecule reacting with the fluorescent-capable ion complexed with the first chelating molecule. A biosensor for monitoring molecular interactions between receptors, including a biosensor having attached thereto a fluorescent-capable ion complexed with a first chelating molecule, whereby upon exposure to a second chelating molecule said complex becomes fluorescent is also provided.
    • 一种用于进行用于监测结合靶的反应的快速均质测定的方法,通过固定衍生化以具有识别结合配体的荧光能螯合物复合物,将标记的第二螯合剂标记在添加到 测定荧光混合螯合物,测定荧光混合螯合物,由此标记物的测定能够监测结合靶的反应。 一种用于执行上述方法的快速测定法,包括第一螯合分子,与第一螯合分子络合的荧光能离子,用于与与第一螯合分子络合的荧光能离子反应的第二螯合分子,以及用于 测量由第二螯合分子产生的荧光与与第一螯合分子络合的具有荧光能力的离子反应。 用于监测受体之间的分子相互作用的生物传感器,包括附着有与第一螯合分子络合的荧光能离子的生物传感器,由此当暴露于第二螯合分子时,还提供所述络合物变为荧光的生物传感器。