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    • 3. 发明申请
    • COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING EVENTS USING TETHERS ANCHORED TO OR ADJACENT TO NANOPORES
    • 用于检测活动的组合物,系统和方法,使用锚固或邻近纳米比亚
    • WO2015187670A3
    • 2016-02-25
    • PCT/US2015033749
    • 2015-06-02
    • ILLUMINA INC
    • MANDELL JEFFREY GGUNDERSON KEVIN LGUNDLACH JENS H
    • C12Q1/00C12Q1/68G01N33/53
    • C12Q1/6869G01N27/44743G01N27/44791C12Q2525/197C12Q2565/607C12Q2565/631
    • Compositions, systems, and methods for detecting events are provided. A composition can include a nanopore including a first side, a second side, and an aperture extending through the first and second sides; and a permanent tether including head and tail regions and an elongated body disposed therebetween. The head region can be anchored to or adjacent to the first or second side of the nanopore. The elongated body including a reporter region can be movable within the aperture responsive to a first event occurring adjacent to the first side of the nanopore. For example, the reporter region is translationally movable toward the first side responsive to the first event, then toward the second side, then toward the first side responsive to a second event. The first event can include adding a first nucleotide to a polynucleotide. The second event can include adding a second nucleotide to the polynucleotide.
    • 提供了用于检测事件的组合,系统和方法。 组合物可以包括纳米孔,其包括第一侧,第二侧和延伸穿过第一和第二侧的孔; 以及包括头部和尾部区域的永久系绳以及设置在它们之间的细长体。 头部区域可以锚固到或邻近纳米孔的第一或第二侧。 包括报告器区域的细长体可响应于邻近纳米孔第一侧发生的第一事件而在孔内可移动。 例如,响应于第一事件,然后朝向第二侧,然后响应于第二事件朝向第一侧,报告器区域可平移地朝向第一侧移动。 第一个事件可以包括向多核苷酸添加第一个核苷酸。 第二个事件可以包括向多核苷酸添加第二个核苷酸。