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1. 发明申请

WO2013182647A2 METHOD FOR DETECTING SUSCEPTIBILITY OF MICROORGANISMS TO CHEMICAL AGENTS 审中-公开
标题翻译：检测微生物对化学试剂的不良性的方法
公开(公告)号：WO2013182647A2
公开(公告)日：2013-12-12
申请号：PCT/EP2013061719
申请日：2013-06-06
申请人： IDMIC SA
发明人： GOVORUN VADIM , ILINA ELENA , PODOPLELOV ALEXEI
CPC分类号： C12Q1/18 , G01N2333/24 , G01N2333/245 , G01N2560/00
摘要： The invention relates to a method for detecting susceptibility of microorganisms to chemical agents, wherein microorganisms are cultured in a nutrient medium broth together with a chemical agent or antibiotic followed by processing of the medium in which microbial cell fragments may be dispensed due to cell lysis, and following that, the microorganism-free supernatant is analyzed on its protein components and compared to control or reference samples. The method comprises the addition of a quantity of microbial cells to a liquid nutrient medium supplemented with a quantity of an enhancer specific for a certain group (genus or family) of microorganisms to the nutrient medium. The microorganisms are pre-incubated and after addition of a quantity of antibiotic to the nutrient medium the microorganisms are again incubated. The cells are removed from the incubated nutrient medium to yield the supernatant. The proteins within the supernatant are extracted by adding alcohol followed by isolation of the proteins. The protein sediment is used to acquire a mass spectrum for comparison with a reference mass spectrum.
摘要翻译：本发明涉及一种用于检测微生物对化学试剂的敏感性的方法，其中将微生物与营养培养基肉汤一起在化学试剂或抗生素中培养，然后加工由于细胞裂解可分泌微生物细胞碎片的培养基，并且随后，对其无微生物的上清液进行蛋白质组分的分析，并与对照或参照样品进行比较。该方法包括将一定数量的微生物细胞添加到补充有一定数量的特定微生物群（属或家族）的增强子的液体营养培养基中的营养培养基中。微生物被预培养，并且在向营养培养基中加入一定数量的抗生素后，再次温育微生物。从培养的营养培养基中除去细胞以产生上清液。通过加入醇提取上清液中的蛋白质，然后分离蛋白质。蛋白质沉淀物用于获得与参考质谱图进行比较的质谱。

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