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1. 发明申请

WO2009109360A1 METHOD OF PROTEASE PRODUCTION IN PLANTS 审中-公开
标题翻译：在植物中生产蛋白质的方法
公开(公告)号：WO2009109360A1
公开(公告)日：2009-09-11
申请号：PCT/EP2009/001502
申请日：2009-03-03
申请人： ICON GENETICS GMBH , KANDZIA, Romy , ENGLER, Carola , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： KANDZIA, Romy , ENGLER, Carola , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8257 , C07K14/415
摘要： A process of producing a protease in a plant or in plant cells, comprising (a) providing a plant comprising a heterologous nucleotide sequence comprising a coding sequence encoding a fusion protein, said fusion protein comprising: an apoplast or plastid signal peptide; a SUMO protein or a derivative of a SUMO protein; and a zymogen of said protease, and (b) expressing said fusion protein.
摘要翻译：一种在植物或植物细胞中产生蛋白酶的方法，包括（a）提供包含异源核苷酸序列的植物，所述异源核苷酸序列包含编码融合蛋白的编码序列，所述融合蛋白包含：质外体或质体信号肽; SUMO蛋白或SUMO蛋白的衍生物; 和所述蛋白酶的酶原，和（b）表达所述融合蛋白。

2. 发明申请

WO2008028661A2 POTEXVIRUS- DERIVED REPLICON 审中-公开
标题翻译： POTEXVIRUS-衍生的REPLICON
公开(公告)号：WO2008028661A2
公开(公告)日：2008-03-13
申请号：PCT/EP2007/007785
申请日：2007-09-06
申请人： ICON GENETICS GMBH , MARILLONNET, Sylvestre , ENGLER, Carola , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , ENGLER, Carola , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8203 , C12N15/8216 , C12N2770/26011 , C12N2770/26043
摘要： Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.
摘要翻译：包含或编码RNA复制品的核酸，其依次包括以下片段（i）至（iii）：i）编码病毒RNA依赖性RNA聚合酶或其功能保守变体的核酸序列; ii）核酸序列，其包含：a）罐状病毒三重基因嵌段或其功能保守变体，和b）编码潜在外壳蛋白或其功能保守变体的序列; 或编码多巴胺病毒运动蛋白的序列; 和iii）在植物或植物组织中可从所述复制品表达的异源核酸序列。

3. 发明申请

WO2006003018A2 BIOLOGICALLY SAFE TRANSIENT PROTEIN EXPRESSION IN PLANTS 审中-公开
标题翻译：生物安全瞬态蛋白表达在植物中
公开(公告)号：WO2006003018A2
公开(公告)日：2006-01-12
申请号：PCT/EP2005/007361
申请日：2005-07-07
申请人： ICON GENETICS AG , MARILLONNET, Sylvestre , ENGLER, Carola , MÜHLBAUER, Stefan , HERZ, Stefan , WERNER, Stefan , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , ENGLER, Carola , MÜHLBAUER, Stefan , HERZ, Stefan , WERNER, Stefan , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82 , A01H5/12 , C12N15/74
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterelogous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译：通过从植物或植物叶中感兴趣的序列表达所述感兴趣的蛋白质来产生感兴趣的蛋白质的方法，包括：a）通过用农杆菌浸润所述植物或所述植物叶来转染所述植物或所述植物叶子所述含有T-DNA的所述农杆菌菌株具有编码复制子的序列部分的恒耳DNA序列，其中所述编码复制子的序列含有所述复制子的复制子功能所需的序列，所述序列衍生自所述复制子表达的所述感兴趣的序列，b）任选地从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供第一遗传修饰呈现所述农杆菌菌株在没有所述补体因子的情况下缺陷用于用所述T-DNA转染生物体。

4. 发明申请

WO2007137788A1 PLANT VIRUS-BASED INDUCIBLE EXPRESSION SYSTEM 审中-公开
标题翻译：植物基于病毒的诱导表达系统
公开(公告)号：WO2007137788A1
公开(公告)日：2007-12-06
申请号：PCT/EP2007/004688
申请日：2007-05-25
申请人： ICON GENETICS GMBH , WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8238
摘要： A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.
摘要翻译：一种产生一种或多于一种感兴趣的蛋白质的方法，包括：（a）提供包含第一异源核苷酸序列的植物或植物细胞，所述第一异源核苷酸序列包含编码RNA复制子的核苷酸序列，和与所述核苷酸序列可操作地连接的第一诱导型启动子编码所述RNA复制子; 所述RNA复制子不编码提供所述植物中所述RNA复制子的细胞与细胞运动的蛋白质; 所述RNA复制子编码聚合酶和所述一种或多种感兴趣的蛋白质，所述聚合酶适于复制所述RNA复制子; 和（b）在步骤（a）的所述植物或植物细胞中诱导所述诱导型启动子，从而在所述植物或植物细胞中产生所述一种或多种感兴趣的蛋白质。

5. 发明申请

WO2004108934A1 SAFE PRODUCTION OF A PRODUCT OF INTEREST IN HYBRID SEEDS 审中-公开
标题翻译：安全生产混合种子利益产品
公开(公告)号：WO2004108934A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/006069
申请日：2004-06-04
申请人： ICON GENETICS AG , WERNER, Stefan , KANDZIA, Romy , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , KANDZIA, Romy , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8242 , C12N15/8257
摘要： A process of the production of a product of interest in an F1 seed obtained by a hybridization of a first and a second transgenic parental plant, said hybridization generating a genetic endowment in said F1 seed for said production by combining in said F1 seed first and second partial genetic endowments of said first and second transgenic parental plants, followed by isolating said product of interest from said F1 seed or a seedling thereof.
摘要翻译：通过第一和第二转基因亲本植物的杂交获得的F1种子中感兴趣的产品的生产过程，所述杂交通过在所述F1种子中组合产生用于所述生产的遗传禀赋第一和第二所述第一和第二转基因亲本植物的部分遗传禀赋，然后从所述F1种子或其幼苗中分离所述目的产物。

6. 发明申请

WO2002077246A3 SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS 审中-公开
公开(公告)号：WO2002077246A3
公开(公告)日：2002-10-03
申请号：PCT/EP2002/003266
申请日：2002-03-22
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
发明人： KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
IPC分类号： C12N15/82
摘要： A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

7. 发明申请

WO2006012906A1 RNA VIRUS-DERIVED PLANT EXPRESSION SYSTEM 审中-公开
标题翻译： RNA病毒衍生植物表达系统
公开(公告)号：WO2006012906A1
公开(公告)日：2006-02-09
申请号：PCT/EP2003/012530
申请日：2003-11-10
申请人： ICON GENETICS AG , ICON GENETICS, INC. , MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8203
摘要： A stably transformed plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, and (ii) a sequence of interest to be expressed from said RNA replicon, whereby said sequences for replicon function exhibit at selected localities of said sequence of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences.
摘要翻译：一种稳定转化的植物，植物部分或植物细胞培养物，其在细胞核中含有具有可操作地连接或可与转录启动子连接的RNA复制子的序列的异源DNA，其中编码RNA复制子的所述序列包含（i）复制子功能序列的所述RNA复制子，所述序列衍生自植物RNA病毒的序列，和（ii）将从所述RNA复制子表达的所需序列，其中所述复制子功能的序列在所述植物的所述序列的选定位置显示与所述植物RNA病毒的所述序列的RNA病毒功能保守差异，与不显示所述差异的RNA复制子相比，所述差异导致复制子形成的频率增加。

8. 发明申请

WO2004067749A1 PLANT TRANSFORMATION WITH IN VIVO ASSEMBLY OF A SEQUENCE OF INTEREST 审中-公开
标题翻译：在生活顺序组合中的植物转化
公开(公告)号：WO2004067749A1
公开(公告)日：2004-08-12
申请号：PCT/EP2004/000892
申请日：2004-01-30
申请人： ICON GENETICS AG , ICON GENETICS, INC. , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8205
摘要： A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest and capable of expressing a function of interest from said DNA sequence of interest, said process comprising (a) providing plant cells or plants with at least two different vectors, whereby (i) said at least two different vectors are adapted to recombine with each other by site-specific recombination in said plant cells for producing a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
摘要翻译：一种生产转基因植物或植物细胞的方法，所述转基因植物或植物细胞在染色体上稳定转化，所述染色体具有感兴趣的DNA序列并且能够从所述感兴趣的DNA序列表达目的功能，所述方法包括（a）向植物细胞或植物提供至少两种不同的载体，其中（i）所述至少两种不同的载体适于通过所述植物细胞中的位点特异性重组而彼此重组，用于产生含有所述感兴趣的DNA序列的非复制重组产物，（ii）所述至少两个不同的载体适于将所述目的DNA序列整合到所述染色体中，（iii）所述感兴趣的DNA序列包含来自所述至少两种不同载体中的至少两种的序列部分，所述序列部分是表达所述感兴趣的功能所必需的从所述感兴趣的DNA序列; 和（b）选择表达所述目的功能的植物或植物细胞。

9. 发明申请

WO2003001900A2 PROCESS OF CONTROLLED SHUFFLING OF CHROMOSOME FRAGMENTS FOR PLANT BREEDING 审中-公开
标题翻译：用于植物育种的染色体片段的控制过程
公开(公告)号：WO2003001900A2
公开(公告)日：2003-01-09
申请号：PCT/EP2002/007134
申请日：2002-06-27
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , ELIBY, Serik , MARILLONNET, Sylvestre , BASCOMB, Newell , GLEBA, Yuri
发明人： KLIMYUK, Victor , ELIBY, Serik , MARILLONNET, Sylvestre , BASCOMB, Newell , GLEBA, Yuri
IPC分类号： A01H
CPC分类号： C12N15/1027
摘要： Disclosed is a method of constructing a wild-species genomic library of chromosome fragments incorporated in a crop-species genome. First, a number of transformants for donor and recipient plant species is produced, carrying the DNA constructs necessary for the exchange of chromosomal fragments mediated by site-specific recombination. The donor and recipient are chosen such that, upon sexual cross or somatic cell fusion, they produce unstable progeny or demonstrate preferential segregation or sorting out. Second, the crossing between donor and recipient species and formation of chromosomal recombinants of donor and recipient plant species is induced. Third, taking advantage of the instability of hybrids between donor and recipient, recombinant cells and plants of the recipient are selected which contain specific chromosome fragments of the donor species. Also disclosed are transgenic plants, libraries and breeding material produced by the methods.
摘要翻译：公开了构建掺入作物品种基因组中的染色体片段的野生物种基因组文库的方法。首先，产生用于供体和受体植物物种的许多转化体，携带用于交换由位点特异性重组介导的染色体片段所必需的DNA构建体。选择供体和受体，使得在性交交或体细胞融合时，它们产生不稳定的后代或证明优先分离或分选。第二，诱导供体和受体物种之间的交叉以及供体和受体植物物种的染色体重组体的形成。第三，利用供体和受体之间的杂交体的不稳定性，选择受体的重组细胞和植物，其含有供体物种的特定染色体片段。还公开了通过该方法生产的转基因植物，文库和育种材料。

10. 发明申请

WO2002096192A2 PROCESS OF PRODUCING ENVIRONMENTALLY SAFE TRANSGENIC ORGANISMS 审中-公开
标题翻译：生产环境安全型转基因生物的方法
公开(公告)号：WO2002096192A2
公开(公告)日：2002-12-05
申请号：PCT/EP2002/004724
申请日：2002-04-29
申请人： ICON GENETICS AG , WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： A01K
CPC分类号： C12N15/8286 , A01K2217/05 , A01K2217/20 , A01K2227/10 , C12N15/8263 , C12N15/8265 , C12N15/8271 , C12N15/8273 , C12N15/8277 , C12N15/85 , C12N15/8509 , C12N2800/30 , C12N2800/40 , C12N2840/10 , C12N2840/445 , Y02A40/162
摘要： A process of producing a transgenic multi-cellular plant or animal organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises hybridising a first multi-cellular organism or a cell thereof having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest and a second multi-cellular organism or a cell thereof having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said hybridation.
摘要翻译：一种产生表达感兴趣的性状并具有所述性状受控分布的后代的转基因多细胞植物或动物生物的方法，其中所述方法包括将具有第一异源DNA序列的第一多细胞生物体或其细胞杂交其包含编码所述感兴趣性状的核苷酸序列的第一片段和具有第二异源DNA序列的第二多细胞生物体或其细胞，所述第二异源DNA序列包含编码所述感兴趣性状的核苷酸序列的第二片段，由此所述第一和第二设计异源序列使得所述感兴趣的性状由于所述杂交后的RNA转拼而产生。

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