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1. 发明申请

WO2003102197A1 TRANSGENIC PLANTS WITH CONTROLLED DISTRIBUTION OF A TRAIT TO PROGENY 审中-公开
标题翻译：具有受控分配的转基因作物的转基因植物
公开(公告)号：WO2003102197A1
公开(公告)日：2003-12-11
申请号：PCT/EP2003/002986
申请日：2003-03-21
申请人： ICON GENETICS AG , ICON GENETICS INC. , WERNER, Stefan , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： ICON GENETICS INC. , WERNER, Stefan , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , C12N15/8213 , C12N15/8216 , C12N15/8231 , C12N15/8265 , C12N15/8289
摘要： A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclearchomosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said firrst heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide sequence. Further, the invention provides a process of producing hybrid seeds for agriculture.
摘要翻译：一种生产表达感兴趣性状的转基因多细胞植物或其部分的方法，所述性状具有所述性状受控于后代的分布，其中所述方法包括（i）产生第一种植物或其细胞，其具有第一种核染色体的位点是包含编码所述目的性状的核苷酸序列的第一片段的第一异源核苷酸序列，（ii）产生第二植物或其细胞，所述第二植物或其细胞在与所述步骤的所述核染色体同源的核小体中具有第二位点（i），第二异源核苷酸序列，其包含编码所述感兴趣性状的核苷酸序列的第二片段，和（iii）将所述第一和第二植物或其细胞杂交以产生表现出所述功能性状的后代，由所述最强异源核苷酸序列编码的蛋白质或多肽和由所述第二h编码的蛋白质或多肽异源核苷酸序列。此外，本发明提供了一种生产用于农业的杂种种子的方法。

2. 发明申请

WO2004067749A1 PLANT TRANSFORMATION WITH IN VIVO ASSEMBLY OF A SEQUENCE OF INTEREST 审中-公开
标题翻译：在生活顺序组合中的植物转化
公开(公告)号：WO2004067749A1
公开(公告)日：2004-08-12
申请号：PCT/EP2004/000892
申请日：2004-01-30
申请人： ICON GENETICS AG , ICON GENETICS, INC. , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8205
摘要： A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest and capable of expressing a function of interest from said DNA sequence of interest, said process comprising (a) providing plant cells or plants with at least two different vectors, whereby (i) said at least two different vectors are adapted to recombine with each other by site-specific recombination in said plant cells for producing a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
摘要翻译：一种生产转基因植物或植物细胞的方法，所述转基因植物或植物细胞在染色体上稳定转化，所述染色体具有感兴趣的DNA序列并且能够从所述感兴趣的DNA序列表达目的功能，所述方法包括（a）向植物细胞或植物提供至少两种不同的载体，其中（i）所述至少两种不同的载体适于通过所述植物细胞中的位点特异性重组而彼此重组，用于产生含有所述感兴趣的DNA序列的非复制重组产物，（ii）所述至少两个不同的载体适于将所述目的DNA序列整合到所述染色体中，（iii）所述感兴趣的DNA序列包含来自所述至少两种不同载体中的至少两种的序列部分，所述序列部分是表达所述感兴趣的功能所必需的从所述感兴趣的DNA序列; 和（b）选择表达所述目的功能的植物或植物细胞。

3. 发明申请

WO2004067748A1 PLANT TRANSFORMATION WITH IN VIVO ASSEMBLY OF A TRAIT 审中-公开
标题翻译：在旅行社组织中的植物转化
公开(公告)号：WO2004067748A1
公开(公告)日：2004-08-12
申请号：PCT/EP2004/000891
申请日：2004-01-30
申请人： ICON GENETICS, AG , ICON GENETICS, INC. , GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5' to 3' direction, for - a 5' part of said RNA sequence of interest and - a 5' part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5' to 3' direction, for - a 3' part of an intron and - a 3' part of said RNA sequence of interest.
摘要翻译：通过表达感兴趣的RNA序列赋予具有感兴趣特征的植物或植物细胞的方法，所述方法包括：向第一载体和第二载体提供所述植物的植物细胞或细胞，并选择赋予所述特征的所述性状的细胞其中所述第一载体含有第一个核苷酸序列，其具有在5'至3'方向上编码所述感兴趣的所述RNA序列的5'部分的第一个区段和内含子的5'部分; 并且所述第二载体含有第二个核苷酸序列，第二个片段在5'至3'方向上编码3'部分的内含子和3'部分所述感兴趣的RNA序列。

4. 发明申请

WO2006079546A3 PRODUCTION OF ANTIBODIES IN PLANTS WITH PLUS -SENSE SINGLE -STRANDED VIRAL RNA VECTORS 审中-公开
公开(公告)号：WO2006079546A3
公开(公告)日：2006-08-03
申请号：PCT/EP2006/000721
申请日：2006-01-27
申请人： ICON GENETICS AG , GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82 , C12N15/67 , C12N15/63
摘要： Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus- sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non- competing viral vectors.

5. 发明申请

WO2004101797A1 PROCESS OF PRODUCING A PLASTID-TARGETED PROTEIN IN PLANT CELLS 审中-公开
标题翻译：在植物细胞中生产PLASTID-TARGEED蛋白的过程
公开(公告)号：WO2004101797A1
公开(公告)日：2004-11-25
申请号：PCT/EP2004/005151
申请日：2004-05-13
申请人： ICON GENETICS AG , KLIMYUK, Victor , BENNING, Gregor , GILS, Mario , GIRITCH, Anatoly , GLEBA, Yuri
发明人： KLIMYUK, Victor , BENNING, Gregor , GILS, Mario , GIRITCH, Anatoly , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C07K14/56 , C07K14/61 , C07K2319/08 , C12N15/8221 , C12N15/8257
摘要： A process of producing a protein of interest in cells of a predetermined plant, comprising: introducing into said cells a vector encoding a fusion protein comprising from the N-terminus to the C-terminus (i) a transit peptide for targeting said fusion protein into plastids and, contiguous thereto, (ii) said protein of interest, wherein the C-terminal three amino acids X -3 X -2 X -1 of said transit peptide and the N-terminal amino acid Z of said protein of interest form a cleavage site X -3 X -2 X -1 -Z for cleaving said fusion protein between X -1 and Z for releasing said protein of interest in plastids, whereby for a predetermined amino acid Z of said protein of interest an amino acid sequence X -3 X -2 X -1 is selected from the set of amino acid sequences X -3 X -2 X -1 naturally occurring contiguous to Z in plastid-targeted fusion proteins in plants, thereby forming said cleavage site.
摘要翻译：一种在预定植物的细胞中产生感兴趣的蛋白质的方法，包括：向所述细胞中引入编码包含N末端至C末端的融合蛋白的载体（i）用于靶向所述融合蛋白的转运肽质体和与其相邻的（ii）所述感兴趣的蛋白质，其中所述转运肽的C末端三个氨基酸X - X - X - 和N-末端氨基酸Z为所述感兴趣的蛋白质形成切割位点X-X-X-Z，用于在X -1和Z之间切割所述融合蛋白，以释放所述质体中的所述目的蛋白，由此，所述目标蛋白质的预定氨基酸Z为氨基酸序列X-X <-2>，选自氨基酸序列X-X X - 1>在植物中的质体靶向融合蛋白中与Z邻接天然存在，从而形成所述切割位点。

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