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1. 发明申请

WO2004067749A1 PLANT TRANSFORMATION WITH IN VIVO ASSEMBLY OF A SEQUENCE OF INTEREST 审中-公开
标题翻译：在生活顺序组合中的植物转化
公开(公告)号：WO2004067749A1
公开(公告)日：2004-08-12
申请号：PCT/EP2004/000892
申请日：2004-01-30
申请人： ICON GENETICS AG , ICON GENETICS, INC. , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8205
摘要： A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest and capable of expressing a function of interest from said DNA sequence of interest, said process comprising (a) providing plant cells or plants with at least two different vectors, whereby (i) said at least two different vectors are adapted to recombine with each other by site-specific recombination in said plant cells for producing a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
摘要翻译：一种生产转基因植物或植物细胞的方法，所述转基因植物或植物细胞在染色体上稳定转化，所述染色体具有感兴趣的DNA序列并且能够从所述感兴趣的DNA序列表达目的功能，所述方法包括（a）向植物细胞或植物提供至少两种不同的载体，其中（i）所述至少两种不同的载体适于通过所述植物细胞中的位点特异性重组而彼此重组，用于产生含有所述感兴趣的DNA序列的非复制重组产物，（ii）所述至少两个不同的载体适于将所述目的DNA序列整合到所述染色体中，（iii）所述感兴趣的DNA序列包含来自所述至少两种不同载体中的至少两种的序列部分，所述序列部分是表达所述感兴趣的功能所必需的从所述感兴趣的DNA序列; 和（b）选择表达所述目的功能的植物或植物细胞。

2. 发明申请

WO2004067748A1 PLANT TRANSFORMATION WITH IN VIVO ASSEMBLY OF A TRAIT 审中-公开
标题翻译：在旅行社组织中的植物转化
公开(公告)号：WO2004067748A1
公开(公告)日：2004-08-12
申请号：PCT/EP2004/000891
申请日：2004-01-30
申请人： ICON GENETICS, AG , ICON GENETICS, INC. , GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5' to 3' direction, for - a 5' part of said RNA sequence of interest and - a 5' part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5' to 3' direction, for - a 3' part of an intron and - a 3' part of said RNA sequence of interest.
摘要翻译：通过表达感兴趣的RNA序列赋予具有感兴趣特征的植物或植物细胞的方法，所述方法包括：向第一载体和第二载体提供所述植物的植物细胞或细胞，并选择赋予所述特征的所述性状的细胞其中所述第一载体含有第一个核苷酸序列，其具有在5'至3'方向上编码所述感兴趣的所述RNA序列的5'部分的第一个区段和内含子的5'部分; 并且所述第二载体含有第二个核苷酸序列，第二个片段在5'至3'方向上编码3'部分的内含子和3'部分所述感兴趣的RNA序列。

3. 发明申请

WO2003102197A1 TRANSGENIC PLANTS WITH CONTROLLED DISTRIBUTION OF A TRAIT TO PROGENY 审中-公开
标题翻译：具有受控分配的转基因作物的转基因植物
公开(公告)号：WO2003102197A1
公开(公告)日：2003-12-11
申请号：PCT/EP2003/002986
申请日：2003-03-21
申请人： ICON GENETICS AG , ICON GENETICS INC. , WERNER, Stefan , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： ICON GENETICS INC. , WERNER, Stefan , GIRITCH, Anatoly , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , C12N15/8213 , C12N15/8216 , C12N15/8231 , C12N15/8265 , C12N15/8289
摘要： A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclearchomosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said firrst heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotide sequence. Further, the invention provides a process of producing hybrid seeds for agriculture.
摘要翻译：一种生产表达感兴趣性状的转基因多细胞植物或其部分的方法，所述性状具有所述性状受控于后代的分布，其中所述方法包括（i）产生第一种植物或其细胞，其具有第一种核染色体的位点是包含编码所述目的性状的核苷酸序列的第一片段的第一异源核苷酸序列，（ii）产生第二植物或其细胞，所述第二植物或其细胞在与所述步骤的所述核染色体同源的核小体中具有第二位点（i），第二异源核苷酸序列，其包含编码所述感兴趣性状的核苷酸序列的第二片段，和（iii）将所述第一和第二植物或其细胞杂交以产生表现出所述功能性状的后代，由所述最强异源核苷酸序列编码的蛋白质或多肽和由所述第二h编码的蛋白质或多肽异源核苷酸序列。此外，本发明提供了一种生产用于农业的杂种种子的方法。

4. 发明申请

WO2002077246A3 SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS 审中-公开
公开(公告)号：WO2002077246A3
公开(公告)日：2002-10-03
申请号：PCT/EP2002/003266
申请日：2002-03-22
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
发明人： KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
IPC分类号： C12N15/82
摘要： A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

5. 发明申请

WO2006012906A1 RNA VIRUS-DERIVED PLANT EXPRESSION SYSTEM 审中-公开
标题翻译： RNA病毒衍生植物表达系统
公开(公告)号：WO2006012906A1
公开(公告)日：2006-02-09
申请号：PCT/EP2003/012530
申请日：2003-11-10
申请人： ICON GENETICS AG , ICON GENETICS, INC. , MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8203
摘要： A stably transformed plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, and (ii) a sequence of interest to be expressed from said RNA replicon, whereby said sequences for replicon function exhibit at selected localities of said sequence of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences.
摘要翻译：一种稳定转化的植物，植物部分或植物细胞培养物，其在细胞核中含有具有可操作地连接或可与转录启动子连接的RNA复制子的序列的异源DNA，其中编码RNA复制子的所述序列包含（i）复制子功能序列的所述RNA复制子，所述序列衍生自植物RNA病毒的序列，和（ii）将从所述RNA复制子表达的所需序列，其中所述复制子功能的序列在所述植物的所述序列的选定位置显示与所述植物RNA病毒的所述序列的RNA病毒功能保守差异，与不显示所述差异的RNA复制子相比，所述差异导致复制子形成的频率增加。

6. 发明申请

WO2003001900A2 PROCESS OF CONTROLLED SHUFFLING OF CHROMOSOME FRAGMENTS FOR PLANT BREEDING 审中-公开
标题翻译：用于植物育种的染色体片段的控制过程
公开(公告)号：WO2003001900A2
公开(公告)日：2003-01-09
申请号：PCT/EP2002/007134
申请日：2002-06-27
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , ELIBY, Serik , MARILLONNET, Sylvestre , BASCOMB, Newell , GLEBA, Yuri
发明人： KLIMYUK, Victor , ELIBY, Serik , MARILLONNET, Sylvestre , BASCOMB, Newell , GLEBA, Yuri
IPC分类号： A01H
CPC分类号： C12N15/1027
摘要： Disclosed is a method of constructing a wild-species genomic library of chromosome fragments incorporated in a crop-species genome. First, a number of transformants for donor and recipient plant species is produced, carrying the DNA constructs necessary for the exchange of chromosomal fragments mediated by site-specific recombination. The donor and recipient are chosen such that, upon sexual cross or somatic cell fusion, they produce unstable progeny or demonstrate preferential segregation or sorting out. Second, the crossing between donor and recipient species and formation of chromosomal recombinants of donor and recipient plant species is induced. Third, taking advantage of the instability of hybrids between donor and recipient, recombinant cells and plants of the recipient are selected which contain specific chromosome fragments of the donor species. Also disclosed are transgenic plants, libraries and breeding material produced by the methods.
摘要翻译：公开了构建掺入作物品种基因组中的染色体片段的野生物种基因组文库的方法。首先，产生用于供体和受体植物物种的许多转化体，携带用于交换由位点特异性重组介导的染色体片段所必需的DNA构建体。选择供体和受体，使得在性交交或体细胞融合时，它们产生不稳定的后代或证明优先分离或分选。第二，诱导供体和受体物种之间的交叉以及供体和受体植物物种的染色体重组体的形成。第三，利用供体和受体之间的杂交体的不稳定性，选择受体的重组细胞和植物，其含有供体物种的特定染色体片段。还公开了通过该方法生产的转基因植物，文库和育种材料。

7. 发明申请

WO2006079546A3 PRODUCTION OF ANTIBODIES IN PLANTS WITH PLUS -SENSE SINGLE -STRANDED VIRAL RNA VECTORS 审中-公开
公开(公告)号：WO2006079546A3
公开(公告)日：2006-08-03
申请号：PCT/EP2006/000721
申请日：2006-01-27
申请人： ICON GENETICS AG , GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： GIRITCH, Anatoly , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82 , C12N15/67 , C12N15/63
摘要： Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus- sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non- competing viral vectors.

8. 发明申请

WO2002077246A2 SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS 审中-公开
标题翻译：使用放大矢量进行定位转换
公开(公告)号：WO2002077246A2
公开(公告)日：2002-10-03
申请号：PCT/EP2002/003266
申请日：2002-03-22
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
发明人： KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8203
摘要： A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.
摘要翻译：将感兴趣的DNA靶向整合到聚集体细胞核基因组中的方法，包括： i）向植物细胞提供能够在植物细胞中自主复制的扩增载体或其前体，所述载体包含： a）编码在植物细胞中起作用的起始起点的DNA序列，b）载体和宿主核DNA之间的位点特异性和/或同源重组所必需的DNA序列，和c）任选的另外的DNA 出于兴趣; ii）任选地提供有助于载体扩增和/或细胞与细胞移动和/或位点特异性和/或同源重排的条件，和iii）选择在植物核DNA中的预定位点进行重组的细胞。

9. 发明申请

WO2004046361A1 METHOD OF CONTROLLING A CELLULAR PROCESS IN A MULTI-CELLULAR ORGANISM 审中-公开
标题翻译：控制多细胞生物体中细胞过程的方法
公开(公告)号：WO2004046361A1
公开(公告)日：2004-06-03
申请号：PCT/EP2003/013021
申请日：2003-11-20
申请人： ICON GENETICS AG , ICON GENETICS, INC. , WERNER, Stefan , MARILLONNET, Sylvestre , DOROKHOV, Yurii , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , MARILLONNET, Sylvestre , DOROKHOV, Yurii , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8216 , C12N15/8217
摘要： A method of controlling a genetically-modified multi-cellular organism or a part thereof, comprising the following steps: (a) providing a multi-cellular organism or a part thereof, whereby cells of said multi-cellular organism or said part contain a heterologous nucleic acid, (b) causing expression of a I protein from said heterologous nucleic acid in at least some of said cells, wherein said protein is capable of (i) leaving a cell and entering other cells of said multi-cellular organism or a part thereof, (ii) causing expression of said protein in cells containing said heterologous nucleic acid, and optionally (iii) controlling a cellular process of interest.
摘要翻译：一种控制遗传修饰的多细胞生物体或其一部分的方法，包括以下步骤：（a）提供多细胞生物体或其一部分，由此所述多细胞生物体或所述部分的细胞含有异源核酸，（b）在至少一些所述细胞中引起来自所述异源核酸的I蛋白的表达，其中所述蛋白质能够（i）离开细胞并进入所述多细胞生物体的其它细胞或部分（ii）引起所述蛋白质在含有所述异源核酸的细胞中的表达，以及任选地（iii）控制感兴趣的细胞过程。

10. 发明申请

WO2004046359A2 METHOD OF CONTROLLING PROCESSES IN PLANTS OR PLANT CELLS 审中-公开
标题翻译：控制植物或植物细胞过程的方法
公开(公告)号：WO2004046359A2
公开(公告)日：2004-06-03
申请号：PCT/EP2003/013016
申请日：2003-11-20
申请人： ICON GENETICS AG , ICON GENETICS, INC. , WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8218
摘要： A method of controlling a genetically-modified plant or plant cells, said methodcomprising the following steps: (a) providing a genetically-modified plant or plant cells, said plant or plant cells containing a heterologous nucleic acid encoding a first polypeptide containing or consisting of a first fragment of a protein, (b) introducing a second polypeptide into cells of said genetically-modified plant or plant cells, said second polypeptide containing (i) a second fragment of said protein and (ii) a peptide sequence enabling the introduction of said second polypeptide into cells of said genetically-modified plant or plant cells, whereby said first fragment and said second fragment jointly generate a predetermined function of said protein only when jointly present.
摘要翻译：所述方法包括以下步骤：（a）提供遗传修饰的植物或植物细胞，所述植物或植物细胞含有编码第一多肽的异源核酸，所述第一多肽含有或由蛋白质的第一片段，（b）将第二多肽引入所述经遗传修饰的植物或植物细胞的细胞中，所述第二多肽含有（i）所述蛋白质的第二片段和（ii）能够引入所述第二多肽进入所述经遗传修饰的植物或植物细胞的细胞，其中所述第一片段和所述第二片段仅在共同存在时共同产生所述蛋白质的预定功能。

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