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1. 发明申请

WO2010004336A2 STIMULATED CELL STANDARDS 审中-公开
标题翻译：刺激细胞标准
公开(公告)号：WO2010004336A2
公开(公告)日：2010-01-14
申请号：PCT/GB2009/050815
申请日：2009-07-09
申请人： HEALTH PROTECTION AGENCY , STEBBINGS, Richard John
发明人： STEBBINGS, Richard John
IPC分类号： G01N33/96
CPC分类号： G01N33/96
摘要： Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labelled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labelled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g. carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation.
摘要翻译：用于产生用于通过刺激细胞在细胞因子释放抑制剂存在下产生细胞因子的细胞内细胞因子水平和细胞因子释放来监测细胞内细胞因子水平和细胞因子释放的方法，用固定剂如多聚甲醛固定刺激的细胞，洗涤至去除多余的固定剂并冷冻干燥受刺激的固定细胞。还公开了用于产生细胞增殖测定的标记参考标准的方法，其中从人或动物体分离的增殖能力的哺乳动物细胞用细胞增殖期间在子细胞之间划分的标记（例如染料）进行标记（例如羧基荧光素琥珀酰亚胺酯），刺激细胞增殖，通过加入固定剂固定增殖的细胞，然后通过冷冻干燥或冷冻保存来保存细胞。

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