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    • 4. 发明申请
    • TRIGGERED ASSEMBLY OF METAFLUOROPHORES
    • 触发组件的METAFLUORORHORES
    • WO2016140726A3
    • 2016-10-27
    • PCT/US2015065948
    • 2015-12-16
    • HARVARD COLLEGE
    • ONG LUVENA LZHANG DAVID YUWEI DIMINGJUNGMANN RALFYIN PENG
    • G01N21/64C12Q1/68
    • C12Q1/6876C07H21/04
    • Aspects of the present disclosure relate to systems, kits and methods that comprise a nucleic acid capture strand linked to a first dye molecule, a nucleic acid trigger strand longer than the capture strand and comprising (a) a capture domain that is complementary to the capture strand and (b) at least two concatenated domains, each of which comprises two subdomains, and a partially double-stranded nucleic acid comprising a single- stranded toehold domain having a nucleotide sequence complementary to one of the subdomains of the two subdomains of the concatenated domains, a double-stranded region linked to a second dye molecule and having a nucleotide sequence complementary to the other of the two subdomains of the concatenated domains, and a single- stranded hairpin loop having a nucleotide sequence that is complementary to the single-stranded toehold domain.
    • 本公开的方面涉及系统,试剂盒和方法,其包含与第一染料分子连接的核酸捕获链,比捕获链长的核酸触发链并且包含(a)与捕获物互补的捕获结构域 (b)至少两个连接的结构域,每个连接结构域包含两个亚结构域,以及包含单链结构域的部分双链核酸,所述单链结构域具有与连接的两个亚结构域的子结构域之一互补的核苷酸序列 与第二染料分子连接并具有与连接域的两个亚域中的另一个互补的核苷酸序列的双链区,以及具有与单链互补的核苷酸序列的单链发夹环 toehold域。