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1. 发明申请

WO2004072291A3 USE OF HOP ACIDS IN FUEL ETHANOL PRODUCTION 审中-公开
标题翻译：在燃料乙醇生产中使用HOP酸
公开(公告)号：WO2004072291A3
公开(公告)日：2005-11-10
申请号：PCT/US2004003684
申请日：2004-02-09
申请人： HAAS INC JOHN I , MAYE JOHN PAUL
发明人： MAYE JOHN PAUL
IPC分类号： A23L3/00 , A23L3/3508 , C12C3/08 , C12C3/12 , C12C11/02 , C12H1/00 , C12P20060101 , C12P7/06 , C13B10/00
CPC分类号： C13B10/006 , A23L3/3508 , C12H1/003 , C12P7/06 , Y02E50/17
摘要： Six hop acids are common to hops and beer: alpha acid, beta acids, isoalpha acids, rho-isoalpha acids, tetrahydro-isoalpha acids, and hexahydro-isoalpha acids. The six hop acids were tested to determine which were the most effective in inhibiting the growth of bacteria common to fuel ethanol production. The bacteria used in the tests were Lactobacillus brevis and Lactobacillus fermentum. The minimum inhibitory concentrations (MIC) of the hop acids were determined using MRS-broth. Molasses mash and wheat mashes were used as the growth media for the fermentations. In all cases the hop acids controlled the growth of these two lactobacillus bacteria with tetrahydroisoalpha acid, hexahydroisoalpha acid, and isoalpha acid killing the most bacteria at the lowest MIC. Treating yeast propagators, steep tanks, and fermenters with a minimum inhibitory concentration of hop acids will stop bacteria growth, increase ethanol yields and avoid the need for antibiotics.
摘要翻译：啤酒花和啤酒中的六跳酸是常见的：α酸，β酸，异亮氨酸，rho-isoalpha酸，四氢异丙酸和六氢异壬酸。测试六滴酸，以确定哪些是抑制燃料乙醇生产中常见的细菌生长最有效的。试验中使用的细菌是短乳杆菌（Lactobacillus brevis）和发酵乳杆菌（Lactobacillus fermentum）。使用MRS-肉汤测定啤酒花酸的最小抑制浓度（MIC）。糖浆和麦芽糖被用作发酵的生长培养基。在所有情况下，啤酒花酸控制这两种乳杆菌细菌的生长，其中四氢异二酸，六氢异
羟酸和异葎草酸以最低的MIC杀死最多的细菌。处理具有最小抑制浓度的啤酒花的酵母繁殖者，陡峭的罐和发酵罐将阻止细菌生长，增加乙醇产量并避免需要抗生素。

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