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1. 发明申请

WO0219963A3 SYNTHETIC ERYTHROPOIESIS STIMULATING PROTEINS 审中-公开
标题翻译：合成红细胞生成素刺激蛋白
公开(公告)号：WO0219963A3
公开(公告)日：2003-02-06
申请号：PCT/US0121928
申请日：2001-07-12
申请人： GRYPHON THERAPEUTICS INC , KOCHENDOERFER GERD , BOTTI PAOLO , BRADBURNE JAMES A , CHEN SHIAH-YUN , CRESSMAN SONYA , HUNTER CHRISTIE L , KENT STEPHEN B H , LOW DONALD W
发明人： KOCHENDOERFER GERD , BOTTI PAOLO , BRADBURNE JAMES A , CHEN SHIAH-YUN , CRESSMAN SONYA , HUNTER CHRISTIE L , KENT STEPHEN B H , LOW DONALD W
IPC分类号： A61K38/00 , A61K38/02 , A61K38/22 , A61K47/34 , A61K47/42 , A61K47/48 , A61P7/00 , A61P7/06 , A61P9/10 , A61P11/02 , A61P11/06 , A61P17/00 , A61P19/02 , A61P29/00 , A61P31/12 , A61P37/06 , A61P37/08 , A61P43/00 , C07K1/00 , C07K1/06 , C07K14/47 , C07K14/505 , C07K14/52 , C07K17/08 , C07K19/00
CPC分类号： C07K14/505 , A61K38/00
摘要： Synthetic erythropoiesis stimulating proteins are provided. Also provided are methods for synthesizing the proteins. The invention further relates to derivatives of such synthetic erythropoiesis stimulating proteins that are polymer-modified in a defined manner. Methods and uses for such proteins and derivatized proteins are also provided.
摘要翻译：提供合成红细胞生成刺激蛋白。还提供了合成蛋白质的方法。本发明还涉及以定义的方式聚合物修饰的这种合成的红细胞生成刺激蛋白的衍生物。还提供了这些蛋白质和衍生化蛋白质的方法和用途。

2. 发明申请

WO0012536A2 LIPID MATRIX-ASSISTED CHEMICAL LIGATION AND SYNTHESIS OF MEMBRANE POLYPEPTIDES 审中-公开
标题翻译：脂质基辅助化学合成和膜多糖的合成
公开(公告)号：WO0012536A2
公开(公告)日：2000-03-09
申请号：PCT/US9919542
申请日：1999-08-26
申请人： GRYPHON SCIENCES , KOCHENDOERFER GERD G , HUNTER CHRISTIE L , KENT STEPHEN B H , BOTTI PAOLO
发明人： KOCHENDOERFER GERD G , HUNTER CHRISTIE L , KENT STEPHEN B H , BOTTI PAOLO
IPC分类号： G01N33/532 , A61K49/08 , A61K51/08 , C07C229/16 , C07K1/00 , C07K1/02 , C07K1/04 , C07K1/107 , C07K1/12 , C07K1/13 , C07K14/11 , C07K14/155 , C07K14/16 , C07K14/195 , C07K14/215 , C07K14/36 , C07K14/435 , C07K14/54 , C07K14/705 , C07K17/02 , C07K19/00 , G01N21/78 , G01N33/542 , G01N33/566 , G01N33/68
CPC分类号： A61K49/0056 , A61K49/0041 , C07C229/16 , C07K1/023 , C07K1/026 , C07K1/04 , C07K1/042 , C07K1/045 , C07K14/005 , C07K14/215 , C07K14/36 , C07K14/43522 , C07K14/5421 , C07K14/705 , C07K2319/00 , C12N2740/16322 , C12N2760/16022 , Y10S435/87
摘要： The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.
摘要翻译：本发明涉及掺入脂质基质中的脂质基质辅助化学连接和膜多肽的合成的方法和组合物。本发明通过逐步化学选择性化学连接未受保护的肽区段，其中至少一个肽片段嵌入脂质基质中，制备嵌入脂质基质内的预折叠膜多肽。可以使用适用于未保护肽段连接的任何化学选择性反应化学物质。合适的脂质基质包括脂质体，胶束，细胞膜片和光学各向同性立体脂质相基质。根据本发明的方法和组合物合成的预先折叠的合成和半合成膜多肽还允许位点特异性引入一个或多个可检测部分，例如发色团，其可以在合成期间方便地引入。本发明的方法和组合物具有多种用途。例如，它们可用于测定配体结合膜多肽和包含受体的结构域，因此对于结构/功能研究，药物筛选/选择/设计和诊断等（包括高通量应用）非常有用。本发明的方法和组合物特别适用于以前不可接近的膜多肽的FRET分析。

3. 发明申请

WO2007140291A3 EXPRESSION QUANTIFICATION USING MASS SPECTROMETRY 审中-公开
标题翻译：使用质谱分析的表达量
公开(公告)号：WO2007140291A3
公开(公告)日：2008-05-29
申请号：PCT/US2007069724
申请日：2007-05-25
申请人： APPLERA CORP , HUNTER CHRISTIE L
发明人： HUNTER CHRISTIE L
IPC分类号： G01N33/68
CPC分类号： G01N33/68 , G01N33/6848 , G01N33/6851 , G01N2333/90209
摘要： In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more iso forms of a protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent.
摘要翻译：在各个方面，本教导提供用于蛋白质表达的绝对定量的系统，方法，测定和试剂盒。在各个方面，本教导提供了确定感兴趣的一种或多种感兴趣的一种或多种感兴趣的蛋白质的浓度的方法。在各个方面，本教导提供使用标记蛋白片段和亲 - 子离子跃迁监测（PDITM）的标准样品测定蛋白质的一种或多种异构体的绝对浓度的方法。在各种实施方案中，可以使用本教导以多种方式确定样品中生物分子的多种同种型，生物过程中的多种蛋白，多种样品的组合或其组合的绝对浓度。在各个方面，提供了评估生物系统对化学试剂的响应的方法。

4. 发明申请

WO2008008919A2 METHODS AND SYSTEMS FOR SEQUENCE-BASED DESIGN OF MULTIPLE REACTION MONITORING TRANSITIONS AND EXPERIMENTS 审中-公开
标题翻译：多元反应监测过程和实验的基于序列设计的方法与系统
公开(公告)号：WO2008008919A2
公开(公告)日：2008-01-17
申请号：PCT/US2007073399
申请日：2007-07-12
申请人： APPLERA CORP , MDS INC , HUNTER CHRISTIE L , PATEL ALPESH A
发明人： HUNTER CHRISTIE L , PATEL ALPESH A
CPC分类号： G06F19/18 , H01J49/004
摘要： Computer-implementable methods consistent with the present teachings can be used in the design of selective- and multiple-reaction monitoring experiments for the identification of proteins and peptides of interest. By determining candidate parent-daughter ion transition pairs and scoring the pairs based on their ability to be detected experimental design time can be reduced and with a more focused list of transition pairs more experiments can be run successfully in a given period of time.
摘要翻译：与本教导一致的计算机可实施的方法可以用于选择性和多重反应监测实验的设计，用于鉴定感兴趣的蛋白质和肽。通过确定候选父 - 子离子转移对，并根据其检测能力对这些对进行评分，可以减少实验设计时间，并且通过更集中的转移对列表，可以在给定的时间段内成功运行更多的实验。

5. 发明申请

WO2007044935A3 METHODS FOR THE DEVELOPMENT OF A BIOMOLECULE ASSAY 审中-公开
标题翻译：生物分子测定方法
公开(公告)号：WO2007044935A3
公开(公告)日：2007-10-18
申请号：PCT/US2006040263
申请日：2006-10-12
申请人： APPLERA CORP , MDS INC , HUNTER CHRISTIE L
发明人： HUNTER CHRISTIE L
IPC分类号： G01N33/68 , G01N30/72
CPC分类号： G01N33/6848 , G01N30/7233 , Y10T436/105831 , Y10T436/24
摘要： The present teachings provide methods for the development of a mass spectrometric based assay for a protein in a sample using parent-daughter ion transition monitoring (PDITM). In various aspects, the present teachings provide methods for developing a mass spectrometric based assay for a protein in a sample without the use of a standard for the protein. In various embodiments, the sample comprises proteolytic fragments of a protein which is present in low abundance in the physiological fluid from which it is derived.
摘要翻译：本教导提供了使用亲 - 子离子跃迁监测（PDITM）开发用于样品中的蛋白质的基于质谱法的测定方法。在各个方面，本教导提供了开发用于样品中蛋白质的基于质谱法的测定方法而不使用蛋白质标准的方法。在各种实施方案中，样品包含蛋白质的蛋白水解片段，所述蛋白质蛋白质在其衍生的生理液体中的丰度低。

6. 发明申请

WO2008151207A3 EXPRESSION QUANTIFICATION USING MASS SPECTROMETRY 审中-公开
标题翻译：使用质谱分析的表达量
公开(公告)号：WO2008151207A3
公开(公告)日：2009-03-05
申请号：PCT/US2008065703
申请日：2008-06-04
申请人： APPLERA CORP , HUNTER CHRISTIE L
发明人： HUNTER CHRISTIE L
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/68 , G01N33/6851 , G01N2333/90209
摘要： In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of about the top forty-one proteins present in human plasma. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more proteins using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the state of a biological system inclduing, but not limited to, the disease state of an animal.
摘要翻译：在各个方面，本教导提供用于蛋白质表达的绝对定量的系统，方法，测定和试剂盒。在各个方面，本教导提供确定人血浆中存在的前四十一个蛋白质的浓度的方法。在各方面，本教导提供了使用标记蛋白片段和亲代 - 子代离子跃迁监测（PDITM）的标准样品来确定一种或多种蛋白质的绝对浓度的方法。在各种实施方案中，可以使用本教导以多重方式确定样品中生物分子的多种同种型，生物过程中的多种蛋白，多种样品的组合或其组合的绝对浓度。在各个方面，提供了评估包括但不限于动物的疾病状态的生物系统的状态的方法。

7. 发明申请

WO2005114700A3 EXPRESSION QUANTIFICATION USING MASS SPECTROMETRY 审中-公开
标题翻译：使用质谱分析的表达量
公开(公告)号：WO2005114700A3
公开(公告)日：2006-02-23
申请号：PCT/US2005017799
申请日：2005-05-19
申请人： APPLERA CORP , HUNTER CHRISTIE L , WEBB SALLY , HUNT ANTONY J , KITTERINGHAM NEIL , PENNINGTON STEPHEN
发明人： HUNTER CHRISTIE L , WEBB SALLY , HUNT ANTONY J , KITTERINGHAM NEIL , PENNINGTON STEPHEN
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/68 , G01N33/6803 , G01N33/6848 , G01N33/6851 , G01N2333/90209 , H01J49/0031
摘要： In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more isoforms of a protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent.
摘要翻译：在各个方面，本教导提供用于蛋白质表达的绝对定量的系统，方法，测定和试剂盒。在各个方面，本教导提供了确定感兴趣的一种或多种感兴趣的一种或多种感兴趣的蛋白质的浓度的方法。在各个方面，本教导提供使用标记蛋白片段和父 - 子离子转移监测（PDITM）的标准样品测定蛋白质的一种或多种同种型的绝对浓度的方法。在各种实施方案中，可以使用本教导以多重方式确定样品中生物分子的多种同种型，生物过程中的多种蛋白，多种样品的组合或其组合的绝对浓度。在各个方面，提供了评估生物系统对化学试剂的响应的方法。

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