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    • 7. 发明申请
    • PROTEASES WITH MODIFIED PRO REGIONS
    • 具有修改的PRO区域的PROTEASES
    • WO2010123754A1
    • 2010-10-28
    • PCT/US2010/031269
    • 2010-04-15
    • DANISCO US INC.FERRARI, EugenioFIORESI, CarolVAN KIMMENADE, Anita
    • FERRARI, EugenioFIORESI, CarolVAN KIMMENADE, Anita
    • C12N15/75C12N9/54
    • C12N9/54C12N15/75
    • The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
    • 本发明提供了用于在细菌宿主细胞中生产成熟蛋白酶的方法和组合物。 所述组合物包括编码经修饰的蛋白酶的修饰的多核苷酸,所述修饰的多核苷酸在所述亲区具有至少一个突变; 由修饰的多核苷酸编码的修饰的丝氨酸蛋白酶; 表达盒,DNA构建体和包含编码修饰的蛋白酶的修饰的多核苷酸的载体; 和用本发明的载体转化的细菌宿主细胞。 所述方法包括用于增强细菌宿主细胞中成熟蛋白酶的产生的方法,例如 芽孢杆菌 宿主细胞。 产生的蛋白酶可用于工业生产酶,适用于各种行业,包括但不限于清洁,动物饲料和纺织加工业。