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1. 发明申请

WO2013086219A1 RIBOSOMAL PROMOTORS FROM B. SUBTILIS FOR PROTEIN PRODUCTION IN MICROORGANISMS 审中-公开
标题翻译：来自B.微生物中蛋白质生产的BTI的RIBOSOMAL PROMOTORS
公开(公告)号：WO2013086219A1
公开(公告)日：2013-06-13
申请号：PCT/US2012/068285
申请日：2012-12-06
申请人： DANISCO US INC. , BONGIORNI, Cristina , FOX, Bryan P. , VAN KIMMENADE, Anita
发明人： BONGIORNI, Cristina , FOX, Bryan P. , VAN KIMMENADE, Anita
IPC分类号： C12N15/75
CPC分类号： C12N9/54 , C12N15/75 , C12Y304/21062
摘要： The present invention provides methods and compositions of improved expression systems in microorganisms. The methods and compositions comprise a ribosomal promoter derived from a Bacillus species microorganism, such a ribosomal RNA or a ribosomal protein promoter.
摘要翻译：本发明提供了微生物中改进的表达系统的方法和组合物。所述方法和组合物包含衍生自芽孢杆菌属物种微生物的核糖体启动子，例如核糖体RNA或核糖体蛋白质启动子。

2. 发明申请

WO2010144283A1 BACILLUS STRAIN FOR INCREASED PROTEIN PRODUCTION 审中-公开
标题翻译：用于增加蛋白质生产的微生物菌株
公开(公告)号：WO2010144283A1
公开(公告)日：2010-12-16
申请号：PCT/US2010/037040
申请日：2010-06-02
申请人： DANISCO US INC. , BONGIORNI, Cristina , FERRARI, Eugenio
发明人： BONGIORNI, Cristina
IPC分类号： C07K14/32 , C12N9/12 , C12N9/16 , C12N1/21
CPC分类号： C12N9/16 , C07K14/32 , C12N9/1205
摘要： The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp . Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp . Host cells is further increased in modified Bacillus sp . Host cells that overexpress YmaH. Methods for producing proteins of interest in the modified host cells are also provided
摘要翻译：本发明提供已被遗传操作以具有增强的产生感兴趣蛋白质的能力的宿主细胞。特别地，本发明涉及改良的芽孢杆菌属具有至少一个失活的phr基因的宿主细胞。通过改良的芽孢杆菌菌种增强生产的目的蛋白质宿主细胞在改良的芽孢杆菌中进一步增加。宿主细胞过度表达YmaH。还提供了在修饰的宿主细胞中产生目标蛋白质的方法

3. 发明申请

WO2018187524A1 BACILLUS HOST CELLS PRODUCING β-GALACTOSIDASES AND LACTASES IN THE ABSENCE OF P-NITROBENZYLESTERASE SIDE ACTIVITY 审中-公开
公开(公告)号：WO2018187524A1
公开(公告)日：2018-10-11
申请号：PCT/US2018/026170
申请日：2018-04-05
申请人： DUPONT NUTRITION BIOSCIENCES APS , BONGIORNI, Cristina
发明人： BONGIORNI, Cristina , KAER, Karina Hansen
IPC分类号： C12R1/07 , C12P1/00
摘要： Certain embodiments of the disclosure are directed to modified Bacillus spp. host cells expressing/producing β-galactosidase and/or lactase enzymes and the use of such enzymes for the production of galactooligosaccharide (GOS) compositions in one or more dairy related end products. More particularly, certain embodiments are directed to expressing/producing such β-galactosidase and/or lactase enzymes in modified Bacillus spp. host cells, wherein such enzymes produced and purified from the modified Bacillus host cells are free of unwanted/undesirable enzymatic side activities including, but not limited to, unwanted/undesirable lipase side activities, phospholipase side activities, side activities, cellulase side activities, pectinase side activities, amylase side activities, protease side activities, mannanase side activities and the like.

4. 发明申请

WO2023023644A1 POLYNUCLEOTIDES ENCODING NOVEL NUCLEASES, COMPOSITIONS THEREOF AND METHODS THEREOF FOR ELIMINATING DNA FROM PROTEIN PREPARATIONS 审中-公开
公开(公告)号：WO2023023644A1
公开(公告)日：2023-02-23
申请号：PCT/US2022/075210
申请日：2022-08-19
申请人： DANISCO US INC.
发明人： BONGIORNI, Cristina , PRATT II, Robert James , REBOLI, Matthew , VAN KIMMENADE, Anita , IVERSON, Amanda Kathleen
IPC分类号： C12N1/08 , C12N9/22 , C12N15/75
摘要： The instant disclosure provides, among other things, novel nuclease proteins (enzymes), novel nuclease compositions and protein preparations thereof, recombinant polynucleotides (DNA) encoding such nuclease proteins, recombinant host cells expressing and producing one or more nuclease proteins (optionally co-expressing one or more proteins of interest) and the like. More particularly, the nuclease proteins (enzymes) of the instant disclosure are particularly useful in mitigating DNA contamination, such as contaminating DNA present in a fermentation broth in which microbial host cells have been fermented, contaminating DNA present in recovered proteins of interest, contaminating DNA present in protein preparations, and the like.

5. 发明申请

WO2021086606A1 MICROBIAL HOST CELLS FOR THE PRODUCTION OF HETEROLOGOUS CYANURIC ACID HYDROLASES AND BIURET HYDROLASES 审中-公开
公开(公告)号：WO2021086606A1
公开(公告)日：2021-05-06
申请号：PCT/US2020/055489
申请日：2020-10-14
申请人： DANISCO US INC
发明人： BARENDS, Sharief , BONGIORNI, Cristina , GUO, Feng , MAGGIO-HALL, Lori, Ann , WACKETT, Lawrence, Philip , WHITED, Gregory, M.
IPC分类号： C12N9/80 , C12N9/86 , C12N15/09 , C12N15/74 , C12P21/02
摘要： The present disclosure is generally related to the fields of biology, molecular biology, genetics, microbial host cells, industrial enzyme production, and the like. More particularly, certain embodiments of the disclosure are related to microbial host cells for the production of heterologous proteins, which microbial host cells are well-suited for growth in submerged cultures for the large-scale production of heterologous cyanuric acid hydrolases and biuret hydrolases.

6. 发明申请

WO2016134213A2 ENHANCED PROTEIN EXPRESSION 审中-公开
标题翻译：增强蛋白表达
公开(公告)号：WO2016134213A2
公开(公告)日：2016-08-25
申请号：PCT/US2016/018598
申请日：2016-02-19
申请人： DANISCO US INC
发明人： BONGIORNI, Cristina , DELANGE, Dennis , ENGLAND, George , KOLKMAN, Marc , LEEFLANG, Chris
IPC分类号： C12N15/68
CPC分类号： C12N15/52 , C03C8/16 , C03C8/18 , C03C17/04 , C03C17/3411 , C09D5/24 , C12N15/67 , C12N15/68 , C12N15/75 , H01B1/22 , H01L31/022425
摘要： The present invention relates in general to nucleic acids and bacterial cells having a genetic alteration that result in increased expression of a protein of interest and methods of making and using the same.
摘要翻译：本发明一般涉及具有导致目标蛋白质表达增加的遗传改变的核酸和细菌细胞，以及制备和使用该蛋白质的方法。

7. 发明申请

WO2017112733A1 ENHANCED PROTEIN PRODUCTION AND METHODS THEREOF 审中-公开
标题翻译：增强的蛋白质生产及其方法
公开(公告)号：WO2017112733A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/067936
申请日：2016-12-21
申请人： DANISCO US INC.
发明人： BONGIORNI, Cristina , NEEF, Jolanda , SCHMIDT, Brian F. , VAN KIMMENADE, Anita , VAN DIJL, Jan Maarten
IPC分类号： C07K14/32 , C12P1/04
CPC分类号： C07K14/32 , C12N9/54 , C12P21/02 , C12Y304/24
摘要： The present disclosure is generally related to modified Gram-positive bacterial cells producing increased amounts of one or more protein(s) of interest. Certain embodiments of the instant disclosure are therefore directed to modified Gram-positive bacterial cells expressing an increased amount of a POI relative to unmodified ( i.e. , parental) Gram-positive bacterial cells, wherein the modified ( i.e. , daughter) bacterial cells comprise a modification which increases ras P gene expression. In certain other embodiments, the disclosure pertains to methods of modifying bacterial cells such that the modified (daughter) cells produce an increased level of a protein of interest. In other embodiments, the disclosure pertains to a protein of interest produced by fermenting a modified bacterial cell of the instant disclosure. Certain other embodiments of the disclosure are directed to one or more proteinaceous compositions comprising one or more protein(s) of interest thus made.
摘要翻译：本公开内容通常涉及产生增加量的一种或多种感兴趣蛋白质的修饰的革兰氏阳性细菌细胞。因此，本公开的某些实施方案涉及相对于未修饰（即，亲本）革兰氏阳性细菌细胞表达增加量的POI的修饰的革兰氏阳性细菌细胞，其中修饰的（

8. 发明申请

WO2017075195A1 ENHANCED PROTEIN EXPRESSION AND METHODS THEREOF 审中-公开
标题翻译：增强的蛋白质表达及其方法
公开(公告)号：WO2017075195A1
公开(公告)日：2017-05-04
申请号：PCT/US2016/059078
申请日：2016-10-27
申请人： DANISCO US INC
发明人： BONGIORNI, Cristina , CERVIN, Marguerite A. , ENGLAND, George , ZHU, Chao , KOOPMAN, Frank Wouter
IPC分类号： C12N1/20 , C12N9/12
CPC分类号： C12N9/1247 , C12P21/00 , C12P21/02
摘要： The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpo C gene encoding a variant RNA-polymerase (RNAP) β'-subunit polypeptide. In certain embodiments, the rpo C gene encoding the variant β'-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpo C gene encoding the variant β-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.
摘要翻译：本公开内容通常涉及产生增加量的一种或多种感兴趣蛋白质和修饰的具有增加的遗传能力的革兰氏阳性细菌细胞的修饰的革兰氏阳性细菌细胞。因此，本公开的某些实施方案涉及相对于表达相同目的蛋白的未修饰（亲本）革兰氏阳性细菌细胞表达增加量的感兴趣蛋白的修饰的革兰氏阳性细菌细胞，其中修饰的细菌细胞包含at 编码变异RNA聚合酶（RNAP）β'-亚基多肽的rpoC基因中的至少一个突变。在某些实施方案中，编码变体β'-亚基多肽的rpoC基因整合到修饰细胞的染色体中。在其他实施方案中，编码变体β-亚基多肽的rpoC基因包含在引入修饰细胞中的染色体外质粒中。在其他实施方案中，本公开涉及感受态芽孢杆菌宿主细胞，其包含编码修饰的rpoC多肽的核酸构建体的至少一个拷贝，所述修饰的rpoC多肽包含与SEQ ID NO：8具有90％序列同一性并且天冬氨酸与甘氨酸取代在位置796 SEQ ID NO：8，其中编码rpoC多肽的多核苷酸对于在引入第一核酸构建体之前是非感受态的芽孢杆菌属宿主细胞是外源的。

9. 发明申请

WO2016100128A1 ENHANCED PROTEIN EXPRESSION 审中-公开
标题翻译：增强蛋白表达
公开(公告)号：WO2016100128A1
公开(公告)日：2016-06-23
申请号：PCT/US2015/065296
申请日：2015-12-11
申请人： DANISCO US INC
发明人： BONGIORNI, Cristina , CHRISTENSEN, Robert, I. , SCHMIDT, Brian, F. , VAN KIMMENADE, Anita
IPC分类号： C12N15/75 , C07K14/32
CPC分类号： C07K14/32 , C12N9/12 , C12N9/54 , C12N9/88 , C12N15/75 , C12P21/02 , C12R1/125
摘要： The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of the Bacillus genus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kin A gene, a phr A gene or a phr E gene.
摘要翻译：本发明一般涉及具有导致目的蛋白质表达增加的遗传改变的细菌细胞和制备和使用这些细胞的方法。本发明的方面包括Grampositive微生物，例如遗传改变的芽孢杆菌的成员，其延迟，减少或阻断用于孢子形成的基因的表达或活化，从而导致目的蛋白质的表达增强。遗传改变是减少kinA基因，phrA基因或phrE基因表达的改变。

10. 发明申请

WO2017152169A1 ENGINEERED RIBOSOMAL PROMOTERS FOR PROTEIN PRODUCTION IN MICROORGANISMS 审中-公开
标题翻译：用于微生物蛋白质生产的工程化RIBOSOMAL启动子
公开(公告)号：WO2017152169A1
公开(公告)日：2017-09-08
申请号：PCT/US2017/020913
申请日：2017-03-06
申请人： DANISCO US INC.
发明人： BONGIORNI, Cristina , KOLKMAN, Marc , LEEFLANG, Chris , RHODIUS, Virgil Arthur , VAN KIMMENADE, Anita
IPC分类号： C07K14/32 , C12N15/75
摘要： The instant disclosure is generally related to novel engineered hybrid ribosomal promoters from Bacillus. In certain embodiments, the instant disclosure is directed to one or more nucleic acid compositions comprising said engineered promoters operably linked to nucleic acids encoding proteins of interest. Thus, the disclosure set forth herein described methods and compositions for the production of proteins of interest using one or more of the novel engineered hybrid ribosomal promoters of the disclosure.
摘要翻译：本公开通常涉及来自芽孢杆菌的新型工程化杂交核糖体启动子。在某些实施方案中，本公开涉及一种或多种包含所述工程化启动子的核酸组合物，其可操作地连接至编码感兴趣蛋白质的核酸。因此，本文阐述的公开内容描述了使用本公开内容的一种或多种新型工程化杂交核糖体启动子来生产感兴趣的蛋白质的方法和组合物。

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