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1. 发明申请

WO2007139470A1 A METHOD OF PREPARING AN IMMOBILISED METAL ION CHROMATOGRAPHY ADSORBENT AND METHODS OF PURIFYING PROTEINS, PEPTIDES OR POLYNUCLEOTIDES. 审中-公开
标题翻译：制备固定化金属离子色谱吸附剂的方法和净化蛋白质，肽或多核苷酸的方法。
公开(公告)号：WO2007139470A1
公开(公告)日：2007-12-06
申请号：PCT/SE2007/000513
申请日：2007-05-28
申请人： GE HEALTHCARE BIO-SCIENCES AB , ANDERSSON, Lars, C. , BELEW, Makonnen , GEBRU, Tesfai , GLAD, Gunnar , LINDGREN, Helena , NORRMAN, Nils
发明人： ANDERSSON, Lars, C. , BELEW, Makonnen , GEBRU, Tesfai , GLAD, Gunnar , LINDGREN, Helena , NORRMAN, Nils
IPC分类号： B01D15/38 , B01J20/289
CPC分类号： B01J20/3265 , B01D15/3828 , B01J20/289 , B01J45/00
摘要： The present invention relates to a method of preparing an immobilised metal ion affinity chromatography (IMAC) adsorbent, which comprises to provide chromatography ligands comprised of alkylene diamine triacetic acid, or a derivative thereof, and coupling thereof to a carrier via nitrogen. In an advantageous embodiment, the alkylene diamine triacetic acid is ethylene diaminetriacetic acid (ED3A). The invention further relates to a method of purifying proteins, peptides and/or polynucleotides, particularily histidine and/or cysteine containing proteins or peptides from an animal cell culture media.
摘要翻译：本发明涉及一种制备固定化金属离子亲和层析（IMAC）吸附剂的方法，其包括提供由亚烷基二胺三乙酸或其衍生物构成的色谱配体，以及通过氮与载体的偶联。在有利的实施方案中，亚烷基二胺三乙酸是乙二胺三乙酸（ED3A）。本发明还涉及从动物细胞培养基中纯化蛋白质，肽和/或多核苷酸，特别是组氨酸和/或含半胱氨酸的蛋白质或肽的方法。

2. 发明申请

WO2003097693A1 METHOD, USE AND KIT FOR SEPARATING ALBUMIN FROM CONTAMINANTS IN A LIQUID 审中-公开
标题翻译：方法，用于从液体中的污染物中分离ALBUM的用途和工具
公开(公告)号：WO2003097693A1
公开(公告)日：2003-11-27
申请号：PCT/SE2003/000792
申请日：2003-05-15
申请人： AMERSHAM BIOSCIENCES AB , BELEW, Makonnen
发明人： BELEW, Makonnen
IPC分类号： C07K14/765
CPC分类号： C07K14/765
摘要： The present invention is a method of separating recombinant human serum albumin (rHSA) from low molecular weight contaminants in a liquid, which method comprises the steps of: (a) providing a separation medium, which is comprised of anion-exchanging ligands coupled to a base matrix; and (b) contacting the liquid with the separation medium to adsorb the rHSA to the ligands. In one embodiment, the functional groups of the ligands are weak anion-exchanging groups, preferably secondary amines, and the density of ligands on the base matrix is relatively high.
摘要翻译：本发明是一种从液体中的低分子量污染物中分离重组人血清白蛋白（rHSA）的方法，该方法包括以下步骤：（a）提供分离介质，其由阴离子交换配体基础矩阵; 和（b）使液体与分离介质接触以将rHSA吸附到配体上。在一个实施方案中，配体的官能团是弱阴离子交换基团，优选仲胺，并且基础基质上配体的密度相对较高。

3. 发明申请

WO2003097692A1 METHOD FOR ALBUMIN PURIFICATION 审中-公开
标题翻译：氧化铝纯化方法
公开(公告)号：WO2003097692A1
公开(公告)日：2003-11-27
申请号：PCT/SE2003/000766
申请日：2003-05-09
申请人： AMERSHAM BIOSCIENCES AB , NORTH CHINA PHARMACEUTICAL GROUP CORPORATION , BELEW, Makonnen , LI, Mei, Yan , ZHANG, Wei
发明人： BELEW, Makonnen , LI, Mei, Yan , ZHANG, Wei
IPC分类号： C07K14/765
CPC分类号： C07K14/765
摘要： The present invention is a method of purifying recombinant human serum albumin (rHSA) from a solution, which method comprises to subject a cell culture supernatant (CCS) comprising rHSA cation exchange on a bimodal high salt tolerant matrix; hydrophobic interaction chromatography (HIC); and anion exchange. The bimodal high salt tolerant cation exchange matrix used enables per-forming a purification of a cell culture supernatant directly, in the sense that no further dilution thereof is necessary. Due to its high binding capacity, said bimodal cation exchange matrix also allows use of a smaller amount of matrix as compared to a corresponding conventional cation exchanger matrix. Accordingly, the present invention allows substantial savings as regards volumes and consequently operation costs.
摘要翻译：本发明是一种从溶液中纯化重组人血清白蛋白（rHSA）的方法，该方法包括在双峰耐盐基质上使包含rHSA阳离子交换的细胞培养上清（CCS）疏水相互作用色谱（HIC）; 和阴离子交换。所使用的双峰高耐盐阳离子交换基质可以直接纯化细胞培养物上清液，因为不需要进一步稀释。由于其结合能力高，与相应的常规阳离子交换剂基质相比，所述双峰阳离子交换基质还允许使用较少量的基质。因此，本发明允许在体积和因此的操作成本方面大量节省。

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