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1. 发明申请

WO2014102368A1 RECOMBINANT HOST CELL FOR BIOSYNTHETIC PRODUCTION 审中-公开
标题翻译：用于生产生产的重组宿主细胞
公开(公告)号：WO2014102368A1
公开(公告)日：2014-07-03
申请号：PCT/EP2013/078117
申请日：2013-12-30
申请人： EVIAGENICS S.A.
发明人： RAMAEN, Odile , SAUVEPLANE, Vincent , PANDJAITAN, Rudy
IPC分类号： C12N9/88 , C12P7/24 , C12N15/52
CPC分类号： C12P7/24 , C12N9/0028 , C12N9/0042 , C12N9/0073 , C12N9/1007 , C12N9/88 , C12N9/93 , C12N15/52 , C12Y105/01036 , C12Y106/02004 , C12Y114/13002 , C12Y114/13007 , C12Y114/13011 , C12Y201/01068 , C12Y301/01073 , C12Y402/01 , C12Y402/01017 , C12Y403/01023 , C12Y403/01024 , C12Y403/01025 , C12Y602/01012
摘要： The invention relates to a cell comprising heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.
摘要翻译：本发明涉及包含编码参与苯丙素代谢途径的多酶复合物的异源多核苷酸的细胞，以及香草醛或其羟基苯甲醛前体的生物合成，所述多酶复合物包含用于生物合成香豆酸和巴豆酸酶的酶。

2. 发明申请

WO2012175570A1 METHOD OF METABOLIC EVOLUTION 审中-公开
标题翻译：代谢演化方法
公开(公告)号：WO2012175570A1
公开(公告)日：2012-12-27
申请号：PCT/EP2012/061877
申请日：2012-06-20
申请人： EVIAGENICS S.A. , PANDJAITAN, Rudy , SEBAI, Sarra , LUQUE, Alejandro
发明人： PANDJAITAN, Rudy , SEBAI, Sarra , LUQUE, Alejandro
IPC分类号： C12N15/10 , C12N15/90 , C12P7/26 , C12P17/06
CPC分类号： C12N15/1058 , C12N15/1027 , C12N15/1082 , C12N15/905 , C12P7/26 , C12P7/40 , C12P7/42 , C12P17/06
摘要： The invention relates to a method for metabolic evolution of a variant of a natural small aromatic molecule product of a metabolic pathway, by somatic in vivo assembly and recombination of said metabolic pathway employing a gene mosaic of at least one gene A, which comprises a) in a single step procedure (i) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (ii) recombining said genes, (iii) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5' end or 3' end anchoring to the 5' or 3' end of said integration site, (iv) recombining eventual further genes of said metabolic pathway, and b) selecting clones comprising said gene mosaic and said eventual further genes capable of expressing said variant, methods of preparing a library of cells producing variants of natural small aromatic molecule products of a metabolic pathway, the libraries so produced and used to prepare said variants.
摘要翻译：本发明涉及代谢途径的天然小芳香族分子产物的变体代谢进化的方法，通过使用至少一种基因A的基因嵌合体的所述代谢途径的体内体内装配和重组，所述方法包括a）在单步骤中（i）将具有序列同源性小于99.5％的至少一个基因A的细胞转化为待重组的另一基因，其是细胞基因组的组成部分或呈现在遗传构建体的框架中，（ii）重组所述基因，（iii）在靶基因组的整合位点产生基因的基因镶嵌，其中所述至少一个基因A在5'末端或3'末端具有单个侧翼靶序列锚定到所述整合位点的5'或3'末端，（iv）重组所述代谢途径的最终进一步的基因，和b）选择包含所述基因嵌合体的克隆和所述最终能够表达所述变体的进一步的基因，方法制备产生代谢途径的天然小芳香族分子产物变体的细胞库，所述文库如此制备并用于制备所述变体。

3. 发明申请

WO2013053883A1 METHOD OF GENERATING GENE MOSAICS IN EUKARYOTIC CELLS 审中-公开
标题翻译：在原核细胞中产生基因组蛋白的方法
公开(公告)号：WO2013053883A1
公开(公告)日：2013-04-18
申请号：PCT/EP2012/070250
申请日：2012-10-12
申请人： EVIAGENICS S.A. , PANDJAITAN, Rudy , LUQUE, Alejandro
发明人： PANDJAITAN, Rudy , LUQUE, Alejandro
IPC分类号： C12N15/90 , C12N15/10
CPC分类号： C12N15/81 , C12N9/0006 , C12N15/1082 , C12N15/905
摘要： The invention relates to a method for generating a gene mosaic by somatic in vivo recombination, comprising a) in a single step procedure (i) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (ii) recombining said genes, (iii) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5' end or 3' end anchoring to the 5´or 3´end of said integration site, and b) selecting clones comprising the gene mosaic, wherein said cell is a eukaryotic strain with a knock-out of at least one DNA repair gene. The invention further refers to a method of producing a diversity of gene mosaics and gene assembly.
摘要翻译：本发明涉及通过体细胞体内重组产生基因嵌合体的方法，其包括a）在单步骤中（i）将具有序列同源性小于99.5％的至少一个基因A的细胞转化至另一基因至重组，其是细胞基因组的组成部分或呈现在遗传构建体的框架中，（ii）重组所述基因，（iii）在靶基因组的整合位点产生基因的基因嵌合，其中所述至少一个基因A具有在所述整合位点的5'端或3'末端的5'端或3'末端具有单个侧翼靶序列，和b）选择包含基因嵌合体的克隆，其中所述细胞是真核生物具有至少一个DNA修复基因的敲除的菌株。本发明还涉及产生多样性的基因马赛克和基因组装的方法。

4. 发明申请

WO2014128252A1 BIOSYNTHESIS OF O-METHYLATED PHENOLIC COMPOUNDS 审中-公开
标题翻译： O-甲基苯酚化合物的生物合成
公开(公告)号：WO2014128252A1
公开(公告)日：2014-08-28
申请号：PCT/EP2014/053412
申请日：2014-02-21
申请人： EVIAGENICS S.A.
发明人： RAMAEN, Odile , SEBAI, Sarra , PANDJAITAN, Rudy
IPC分类号： C12N9/10 , C12N15/52 , C12P7/22 , C12P7/42 , C12P7/24
CPC分类号： C12N9/1011 , C12N15/52 , C12P7/22 , C12P7/24 , C12P7/42
摘要： The invention provides for a process for biosynthesis of an O-methylated compound by biotransforming a compound of Formula I into an O-methylated compound, wherein R 1 is H, CHO, COOH, C 1-6 alkyl-COOH, or C 2-6 alkenyl-COOH; R 2 is H, OH, or OCH 3 ; which process comprises the steps: a) providing a recombinant host cell comprising i) at least one first heterologous gene encoding a first enzyme which is a 4-O-1 methyltransferase operable to biotransform the compound into a 4-O-methylated compound; and ii) optionally, if R 2 is OH, at least one second heterologous gene encoding a second enzyme which is a 3-O-methyltransferase, wherein said first and second enzymes are operable to biotransform the compound into a 3,4-O-dimethylated compound; b) cultivating said host cell to express said first and optionally said second enzyme; c) providing the compound and biotransforming the compound into a 4-O-methylated compound or if R 2 is OH, into a 3,4-O-dimethylated compound.
摘要翻译：本发明提供了通过将式I化合物生物转化成O-甲基化化合物的O-甲基化化合物的生物合成方法，其中R 1是H，CHO，COOH，C 1-6烷基-COOH或C 2-6烯基-COOH ; R2是H，OH或OCH3; 所述方法包括以下步骤：a）提供重组宿主细胞，其包含i）至少一种编码第一种酶的第一异源基因，所述第一种酶是可操作以将化合物生物转化为4-O-甲基化化合物的4-O-1甲基转移酶; 和ii）任选地，如果R 2是OH，则至少一个编码作为3-O-甲基转移酶的第二种酶的第二种异源基因，其中所述第一种和第二种酶可操作以将化合物生物转化为3,4-二甲基化的复合; b）培养所述宿主细胞以表达所述第一和任选所述第二酶; c）提供化合物并将化合物生物转化成4-O-甲基化的化合物，或者如果R2是OH，则转化为3,4-O-二甲基化的化合物。

5. 发明申请

WO2011124693A1 METHOD OF GENERATING GENE MOSAICS 审中-公开
标题翻译：产生基因组蛋白的方法
公开(公告)号：WO2011124693A1
公开(公告)日：2011-10-13
申请号：PCT/EP2011/055530
申请日：2011-04-08
申请人： EVIAGENICS S.A. , PANDJAITAN, Rudy , LUQUE, Alejandro
发明人： PANDJAITAN, Rudy , LUQUE, Alejandro
IPC分类号： C12N15/10 , C12N15/90
CPC分类号： C12N15/1058 , C12N15/1027 , C12N15/1082 , C12N15/90 , C12N15/902
摘要： The invention relates to a method for generating a gene mosaic by somatic in vivo recombination, comprising: e) in a single step procedure (vii) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (viii) recombining said genes, (ix) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5' end or 3' end anchoring to the 5'or 3'end of said integration site, and f) selecting clones comprising the gene mosaic, as well as a method of producing a diversity of gene mosaics and gene assembly.
摘要翻译：本发明涉及通过体细胞体内重组产生基因嵌合体的方法，其包括：e）在单步骤过程中（vii）将具有序列同源性小于99.5％的至少一个基因A的细胞转化至另一基因重组，其是细胞基因组的组成部分或呈现在遗传构建体的框架中，（viii）重组所述基因，（ix）在靶基因组的整合位点产生基因的基因嵌合体，其中所述至少一个基因A在所述整合位点的5'端或3'端的5'端或3'末端具有单个侧翼靶序列，和f）选择包含基因嵌合体的克隆，以及方法产生多样性的基因马赛克和基因组装。

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