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1. 发明申请

WO2008122447A3 DNA METHYLATION ASSAY FOR THE DIAGNOSIS OR PROGNOSIS OF MEDICAL CONDITIONS 审中-公开
标题翻译：用于诊断或预防医学条件的DNA甲基化测定
公开(公告)号：WO2008122447A3
公开(公告)日：2009-05-07
申请号：PCT/EP2008002850
申请日：2008-04-10
申请人： EPIGENOMICS AG , MODEL FABIAN , SLEDZIEWSKI ANDREW , DE VOS THEO , SCHUSTER MATTHIAS , DISTLER JUERGEN , LOFTON-DAY CATHERINE
发明人： MODEL FABIAN , SLEDZIEWSKI ANDREW , DE VOS THEO , SCHUSTER MATTHIAS , DISTLER JUERGEN , LOFTON-DAY CATHERINE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2523/125 , C12Q2600/154
摘要： The present invention relates to methods for performing DNA methylation assays of biological samples for features associated with disease conditions. The method is carried out on 2-8 aliquots of the =sample. The extent of methylation is used in the prediction of a medical condition. Among the conditions analysed are colorectal and breast cancer and among the genes analysed are SEPT9 and PITX2.
摘要翻译：本发明涉及用于与疾病状况相关的特征进行生物样品的DNA甲基化测定的方法。该方法在2-8等分试样上进行。甲基化的程度用于预测医疗状况。所分析的病例包括结肠直肠癌和乳腺癌，分析的基因有SEPT9和PITX2。

2. 发明申请

WO2008008983A2 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：WO2008008983A2
公开(公告)日：2008-01-17
申请号：PCT/US2007073509
申请日：2007-07-13
申请人： EPIGENOMICS AG , LOFTON-DAY CATHY , SLEDZIEWSKI ANDREW , LESCHE RALF , SCHUSTER MATTHIAS , DISTLER JUERGEN , TETZNER REIMO , HILDMANN THOMAS , MODEL FABIAN , SONG XIAOLING
发明人： LOFTON-DAY CATHY , SLEDZIEWSKI ANDREW , LESCHE RALF , SCHUSTER MATTHIAS , DISTLER JUERGEN , TETZNER REIMO , HILDMANN THOMAS , MODEL FABIAN , SONG XIAOLING
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/154
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供用于检测或检测和区分增殖性疾病之间或之中的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

3. 发明申请

WO2007115213A2 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：方法和核酸分析细胞增殖性疾病
公开(公告)号：WO2007115213A2
公开(公告)日：2007-10-11
申请号：PCT/US2007065706
申请日：2007-03-30
申请人： EPIGENOMICS AG , LOFTON-DAY CATHY , SLEDZIEWSKI ANDREW , LESCHE RALF , SCHUSTER MATTHIAS , DISTLER JUERGEN , HILDMANN THOMAS , MODEL FABIAN , SONG XIAOLING , TETZNER REIMO
发明人： LOFTON-DAY CATHY , SLEDZIEWSKI ANDREW , LESCHE RALF , SCHUSTER MATTHIAS , DISTLER JUERGEN , HILDMANN THOMAS , MODEL FABIAN , SONG XIAOLING , TETZNER REIMO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/154
摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供了用于检测或检测和区分肝细胞增殖性病症或用于检测或检测和区分结直肠细胞增殖性病症或在结肠直肠细胞增殖性病症中进行区分的方法，核酸和试剂盒。本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别之间的检测和分化，从而使得改善的患者诊断和治疗成为可能。

4. 发明申请

WO2006113466A8 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：WO2006113466A8
公开(公告)日：2012-04-12
申请号：PCT/US2006014131
申请日：2006-04-17
申请人： EPIGENOMICS AG , DISTLER JUERGEN , HILDMANN THOMAS , LESCHE RALF , LOFTON-DAY CATHY , MODEL FABIAN , SCHUSTER MATTHIAS , SLEDZIEWSKI ANDREW , SONG XIAOLING , TETZNER REIMO
发明人： DISTLER JUERGEN , HILDMANN THOMAS , LESCHE RALF , LOFTON-DAY CATHY , MODEL FABIAN , SCHUSTER MATTHIAS , SLEDZIEWSKI ANDREW , SONG XIAOLING , TETZNER REIMO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

5. 发明申请

WO2006113466A3 METHODS AND NUCLEIC ACIDS FOR ANALYSES OF CELLULAR PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于细胞增殖性疾病分析的方法和核酸
公开(公告)号：WO2006113466A3
公开(公告)日：2007-01-18
申请号：PCT/US2006014131
申请日：2006-04-17
申请人： EPIGENOMICS INC , DISTLER JUERGEN , HILDMANN THOMAS , LESCHE RALF , LOFTON-DAY CATHY , MODEL FABIAN , SCHUSTER MATTHIAS , SLEDZIEWSKI ANDREW , SONG XIAOLING , TETZNER REIMO
发明人： DISTLER JUERGEN , HILDMANN THOMAS , LESCHE RALF , LOFTON-DAY CATHY , MODEL FABIAN , SCHUSTER MATTHIAS , SLEDZIEWSKI ANDREW , SONG XIAOLING , TETZNER REIMO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6806 , C12Q2600/106 , C12Q2600/112 , C12Q2600/154 , C12Q2600/158
摘要： Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明的方面提供用于检测或检测和区分肝细胞增殖性疾病或用于检测或检测和鉴别结直肠细胞增殖性疾病之间或之间的方法，核酸和试剂盒。具体方面公开并提供基因组序列，其甲基化模式具有用于改善所述疾病类别的检测和分化的实质性用途，从而能够改善患者的诊断和治疗。

6. 发明申请

WO2009065511A2 METHODS AND NUCLEIC ACIDS FOR THE ANALYSIS OF GENE EXPRESSION ASSOCIATED WITH THE DEVELOPMENT OF PROSTATE CELL PROLIFERATIVE DISORDERS 审中-公开
标题翻译：用于分析与前列腺细胞增殖性疾病发展相关的基因表达的方法和核酸
公开(公告)号：WO2009065511A2
公开(公告)日：2009-05-28
申请号：PCT/EP2008009510
申请日：2008-11-11
申请人： EPIGENOMICS AG , SLEDZIEWSKI ANDREW , PAYNE SHANNON , SCHUSTER MATTHIAS , LEWIN JOERN , SCHLEGEL THOMAS , MOROTTI ANDREW M
发明人： SLEDZIEWSKI ANDREW , PAYNE SHANNON , SCHUSTER MATTHIAS , LEWIN JOERN , SCHLEGEL THOMAS , MOROTTI ANDREW M
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , C12Q2600/154 , C12Q2600/158
摘要： The invention provides methods, nucleic acids and kits for detecting prostate cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said disorder, thereby enabling the improved diagnosis and treatment of patients.
摘要翻译：本发明提供了用于检测前列腺细胞增殖性病症的方法，核酸和试剂盒。本发明公开了其甲基化模式可用于改善所述疾病的检测的基因组序列，从而使患者的改善的诊断和治疗成为可能。

7. 发明申请

WO2006039563A2 METHOD FOR PROVIDING DNA FRAGMENTS DERIVED FROM AN ARCHIVED SAMPLE 审中-公开
标题翻译：用于提供从存档样品衍生的DNA片段的方法
公开(公告)号：WO2006039563A2
公开(公告)日：2006-04-13
申请号：PCT/US2005035317
申请日：2005-09-30
申请人： EPIGENOMICS AG , BALLHAUSE MATTHIAS , BERLIN KURT , DIETRICH DIMO , KLUTH ANTJE , SCHUSTER MATTHIAS , WAGNER UTE , WASSERKORT REINHOLD , ZIEBARTH HEIKE
发明人： BALLHAUSE MATTHIAS , BERLIN KURT , DIETRICH DIMO , KLUTH ANTJE , SCHUSTER MATTHIAS , WAGNER UTE , WASSERKORT REINHOLD , ZIEBARTH HEIKE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1003 , C12Q2527/127 , C12Q2523/125 , C12Q2521/531 , C12Q2547/107
摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.
摘要翻译：本发明的方面涉及从归档样品（例如石蜡包埋和/或固定组织活组织检查等）提供DNA片段的组合物和方法。具体方面提供了分离DNA的高产率以及大部分DNA由长DNA片段组成并且分离的基因组DNA不含相关或交联的污染物如蛋白质，肽，氨基酸或RNA的方法。这些方法是容易的，具有成本效益的，其特征在于重现性和可靠性高。特定方面提供用于提供衍生自归档样品的DNA片段的方法，其中在例如扩增步骤之前的DNA的产量为至少20％，并且可扩增至约1000个碱基对的扩增子。

8. 发明申请

WO2005098035A3 METHOD FOR THE QUANTIFICATION OF METHYLATED DNA 审中-公开
标题翻译：甲基化DNA的定量方法
公开(公告)号：WO2005098035A3
公开(公告)日：2006-02-02
申请号：PCT/EP2005003793
申请日：2005-04-06
申请人： EPIGENOMICS AG , GUETIG DAVID , HABIGHORST DIRK , KLUTH ANTJE , SCHMITT ARMIN , SCHUSTER MATTHIAS , SCHWOPE INA
发明人： GUETIG DAVID , HABIGHORST DIRK , KLUTH ANTJE , SCHMITT ARMIN , SCHUSTER MATTHIAS , SCHWOPE INA
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6823 , C12Q2565/101 , C12Q2535/131 , C12Q2545/114 , C12Q2523/125
摘要： The method according to the invention concerns a method for the quantification of two different variations of a DNA sequence. Particularly, the invention relates to a quantification of methylated DNA. For this purpose, the DNA to be investigated is first converted so that cytosine is converted to uracil, while 5-methylcytosine remains unchanged. Then the converted DNA is amplified by means of a real-time PCR. However, probes are utilized, one of which is specific for the methylated status and one for the unmethylated status of the DNA. The degree of methylation of the investigated DNA can be calculated from the ratio of the signal intensities of the probes or from the Ct values. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer and other disorders associated with a change in the methylation status, as well as for the prediction of drug interactions.
摘要翻译：根据本发明的方法涉及用于量化DNA序列的两个不同变体的方法。特别地，本发明涉及甲基化DNA的定量。为此目的，首先将待研究的DNA转化为胞嘧啶转化为尿嘧啶，而5-甲基胞嘧啶保持不变。然后通过实时PCR扩增转化的DNA。然而，使用探针，其中之一是特异于甲基化状态，一个用于DNA的非甲基化状态。所研究的DNA的甲基化程度可以根据探针的信号强度或Ct值的比值来计算。根据本发明的方法特别适合于与甲基化状态的变化相关的癌症和其它疾病以及用于预测药物相互作用的诊断和预后。

9. 发明申请

WO2008009365A2 A METHOD FOR DETERMINING THE METHYLATION RATE OF A NUCLEIC ACID 审中-公开
标题翻译：一种确定核酸甲基化速率的方法
公开(公告)号：WO2008009365A2
公开(公告)日：2008-01-24
申请号：PCT/EP2007006064
申请日：2007-07-09
申请人： EPIGENOMICS AG , LEWIN JOERN , LESCHE RALF , SCHUSTER MATTHIAS , DIETRICH DIMO
发明人： LEWIN JOERN , LESCHE RALF , SCHUSTER MATTHIAS , DIETRICH DIMO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6869 , C12Q2523/125 , C12Q2535/107 , C12Q2545/101
摘要： The invention relates to a method for quantitatively determining the methylation rate of a nucleic acid through sequencing. According to the invention, the method comprises at least the following steps: a) treating the nucleic acid with a chemical reagent or an enzyme containing solution, whereby the base pairing behavior of methylated cytosine bases and/or unmethylated cytosine bases of the nucleic acid are altered such that methylated cytosine bases become distinguishable from unmethylated cytosine bases, and b) introducing into the nucleic acid at least one base for generating a sequencing signal to be used as a reference signal for normalization, and c) sequencing the nucleic acid, whereby a signal from each cytosine base of the nucleic acid, or a signal from each guanine base of the nucleic acid and a reference signal from the at least on introduced base is obtained, and d) normalizing the signal obtained from each cytosine base of the nucleic acid, or the signal obtained from each guanine base of the nucleic acid to the reference signal from the at least one introduced base.
摘要翻译：本发明涉及通过测序定量测定核酸甲基化速率的方法。根据本发明，该方法至少包括以下步骤：a）用化学试剂或含酶溶液处理核酸，由此核酸的甲基化胞嘧啶碱基和/或非甲基化胞嘧啶碱基的碱基配对行为是改变为使得甲基化胞嘧啶碱基与非甲基化胞嘧啶碱基区分开，并且b）将至少一个碱基引入至少一个碱基，用于产生用作标准化参考信号的测序信号，和c）对核酸进行测序，由此获得来自核酸的每个胞嘧啶碱基的信号或来自核酸的每个鸟嘌呤碱基的信号和来自至少一个引入的碱基的参考信号，以及d）使从核酸的每个胞嘧啶碱基获得的信号归一化或从核酸的每个鸟嘌呤碱基获得的信号与来自至少一个引入的碱基的参考信号。

10. 发明申请

WO2004022779A3 METHOD FOR THE DETECTION OF NUCLEIC ACID SEQUENCES BY MEANS OF CRACKABLE PROBE MOLECULES 审中-公开
标题翻译：通过可纺固体分子检测核酸序列的方法
公开(公告)号：WO2004022779A3
公开(公告)日：2004-06-03
申请号：PCT/DE0302936
申请日：2003-09-01
申请人： EPIGENOMICS AG , SCHATZ PHILIPP , SCHUSTER MATTHIAS , BERLIN KURT
发明人： SCHATZ PHILIPP , SCHUSTER MATTHIAS , BERLIN KURT
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2563/167
摘要： The invention relates to a method for detecting nucleic acid sequences, which is characterized by the fact that the following steps are carried out: a) at least one nucleic acid is bound onto a solid phase; b) probe molecules which are provided with a crackable bond and a probe molecule-specific mass marker are hybridized in a sequence-specific manner to the nucleic acid; c) the probe molecules that are not hybridized are removed; d) the hybridized probe molecules are brought into contact with a matrix which cracks said crackable bonds and is used as a matrix in a MALDI mass spectrometer; e) the mass markers are detected in the positions in which the nucleic acid is bound.
摘要翻译：本发明描述了用于检测核酸序列的方法，其特征在于进行以下步骤：a）至少一种核酸与固相结合; b）探针分子与核酸序列特异性杂交，其中探针分子具有可剪切键和对探针分子特异的质量标签; c）去除未杂交的探针分子; d）使杂交的探针分子与裂解所述可裂解键的基质接触，同时用作MALDI质谱仪中的基质; e）检测核酸结合位置的质量标签。

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