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1. 发明申请

WO2006079814A2 REACTION METHOD 审中-公开
标题翻译：反应方法
公开(公告)号：WO2006079814A2
公开(公告)日：2006-08-03
申请号：PCT/GB2006/000264
申请日：2006-01-26
申请人： ENIGMA DIAGNOSTICS LTD , SQUIRREL, David, James , LEE, Martin, Alan
发明人： SQUIRREL, David, James , LEE, Martin, Alan
IPC分类号： G01N35/00 , B01J19/00 , B01L3/00
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00335 , B01J2219/00344 , B01J2219/00421 , B01J2219/00468 , B01J2219/00704 , B01J2219/00722 , B01L3/502 , B01L3/5021 , B01L7/52 , B01L2200/16 , B01L2300/0838 , B01L2300/087 , B01L2400/0409 , C40B40/06 , G01N35/0098
摘要： A method for carrying out a multi-step reaction, said method comprising 1) adding one or more first reagents to a reaction vessel, said reaction vessel comprising an upper chamber capable of holding reagents, which is open to a lower chamber to which reagent flow is restricted, 2) subjecting said reaction vessel to a centrifugal force so as to drive the said one or more first reagents into the lower chamber; 3) adding a further reagent to the first chamber and closing said chamber; 4) subjecting at least one of the lower chamber or the upper chamber to conditions which cause said one or more first reagents or said further reagent to take part in a first reaction or reach a desired reaction condition; 5) subjecting said reaction vessel to a centrifugal force so as to drive the said further reagent into the lower chamber and allowing it to interact with the contents of the lower chamber; wherein at least steps (2) to (5) are carried out automatically . This method allows multiple steps to be carried out in vessels without opening the vessel. It is particularly suitable for use in connection with PCR reactions.
摘要翻译：一种进行多步反应的方法，所述方法包括：1）向反应容器中加入一种或多种第一试剂，所述反应容器包括能容纳试剂的上室，所述反应容器向下室开放，试剂流 2）对所述反应容器进行离心力，以驱动所述一种或多种第一试剂进入下室; 3）向第一室加入另外的试剂并关闭所述室; 4）使下室或上室中的至少一个使得使所述一种或多种第一试剂或所述另外的试剂参与第一反应或达到所需反应条件的条件; 5）使所述反应容器受到离心力，以便将所述另外的试剂驱动到下室中并允许其与下室的内容物相互作用; 其中至少步骤（2）至（5）自动进行。该方法允许在不打开容器的情况下在容器中进行多个步骤。特别适用于PCR反应。

2. 发明申请

WO2004065017A2 APPARATUS FOR COLLECTING PARTICLES 审中-公开
标题翻译：收集颗粒的装置
公开(公告)号：WO2004065017A2
公开(公告)日：2004-08-05
申请号：PCT/GB2004/000251
申请日：2004-01-21
申请人： THE SECRETARY OF STATE FOR DEFENCE , SYMONDS, William, Hunter , SQUIRREL, David, James , POMEROY, Nigel, Patrick , BERRY, Martin, John
发明人： SYMONDS, William, Hunter , SQUIRREL, David, James , POMEROY, Nigel, Patrick , BERRY, Martin, John
IPC分类号： B04C5/20
CPC分类号： B01D45/16 , B01D50/004 , B04C3/02 , B04C5/18 , B04C5/20 , B04C9/00 , B04C11/00 , G01N1/2211 , G01N15/06 , G01N2001/2223 , G01N2015/0088 , G01N2015/0681
摘要： Improved apparatus for collecting particles from air comprises a cyclone having air inlet and outlet means, means maintaining an airflow there through and means delivering a collecting fluid from a reservoir to particles separated from the air flow in the cyclone, in which the air inlet means is associated with means for heating the incoming air. The apparatus, which is optionally provided with enclosure means for trapping heat, prevents freezing of the collecting fluid at temperatures near to and even below 0 ° C.
摘要翻译：用于从空气中收集颗粒的改进的装置包括具有空气入口和出口装置的旋风器，意味着保持其中的气流，以及将收集流体从储存器输送到与旋风分离器中的空气流分离的颗粒的装置，其中进气装置与用于加热进入空气的装置相关联。可选地设置有用于捕获热量的封闭装置的装置防止在接近甚至低于0℃的温度下收集液体的冷冻。

3. 发明申请

WO2003042693A2 SIGNAL SYSTEM AND ELEMENTS USED THEREIN 审中-公开
标题翻译：信号系统和使用的元件
公开(公告)号：WO2003042693A2
公开(公告)日：2003-05-22
申请号：PCT/GB2002/005120
申请日：2002-11-13
申请人： THE SECRETARY OF STATE FOR DEFENCE , DAY, John, Cavendish , SQUIRREL, David, James , BAILEY, Mark, John , WHITE, Peter, John
发明人： DAY, John, Cavendish , SQUIRREL, David, James , BAILEY, Mark, John , WHITE, Peter, John
IPC分类号： G01N33/50
CPC分类号： C12N9/0083 , C07K14/43563 , C07K2319/00 , C12Q1/66
摘要： A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.
摘要翻译：一种测量细胞内ATP和/或基因表达的方法，该方法包括用编码萤光素酶和荧光素再循环蛋白的构建体转化细胞，将荧光素引入所述细胞，以及监测来自所述细胞的生物发光信号。还描述了新的萤光素回收蛋白，并且这些蛋白质以及它们在光学活性酶底物生产中的应用被要求保护。

4. 发明申请

WO2003042693A3 METHOD FOR MEASURING INTRACELLULAR ATP AND/OR GENE EXPRESSION 审中-公开
公开(公告)号：WO2003042693A3
公开(公告)日：2003-05-22
申请号：PCT/GB2002/005120
申请日：2002-11-13
申请人： THE SECRETARY OF STATE FOR DEFENCE , DAY, John, Cavendish , SQUIRREL, David, James , BAILEY, Mark, John , WHITE, Peter, John
发明人： DAY, John, Cavendish , SQUIRREL, David, James , BAILEY, Mark, John , WHITE, Peter, John
IPC分类号： G01N33/50
摘要： A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.

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