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1. 发明申请

WO2010000416A1 PROCESS FOR THE EXTRACTION OF FATTY ACIDS FROM ALGAL BIOMASS 审中-公开
标题翻译：从ALGAL BIOMASS提取脂肪酸的方法
公开(公告)号：WO2010000416A1
公开(公告)日：2010-01-07
申请号：PCT/EP2009/004617
申请日：2009-06-19
申请人： ENI S.P.A. , D'ADDARIO, Ezio, Nicola , CAPUANO, Federico , D'ANGELI, Edoardo , MEDICI, Roberto
发明人： D'ADDARIO, Ezio, Nicola , CAPUANO, Federico , D'ANGELI, Edoardo , MEDICI, Roberto
IPC分类号： C11B1/10 , C11C1/04 , C12M1/107 , C12M1/00 , C12P7/64 , C11C3/00
CPC分类号： C11B1/10 , C11C1/04 , C11C3/003 , C12P7/6409 , Y02E50/13 , Y02E50/343
摘要： Process for the extraction of fatty acids from algal biomass comprising: producing an aqueous suspension of algal biomass; subjecting the aqueous suspension of algal biomass to acid hydrolysis and extraction by the addition of at least one non-polar organic solvent and at least one inorganic acid to said aqueous suspension of algal biomass, so as to obtain the following three phases: (i) a semisolid phase comprising a slurry of the algal biomass; (ii) an aqueous phase comprising inorganic compounds and hydrophilic organic compounds; (iii) an organic phase comprising fatty acids and hydrophobic organic compounds other than said fatty acids.
摘要翻译：从藻类生物质中提取脂肪酸的方法，包括：产生藻类生物质的水悬浮液; 通过向所述藻类生物质的水悬浮液中加入至少一种非极性有机溶剂和至少一种无机酸使藻类生物质的水悬浮液进行酸水解和提取，以获得以下三个阶段：（i）包含藻类生物质的浆料的半固体相; （ii）包含无机化合物和亲水性有机化合物的水相; （iii）包含除所述脂肪酸以外的脂肪酸和疏水性有机化合物的有机相。

2. 发明申请

WO2010089063A1 PROCESS FOR THE EXTRACTION OF LIPIDS FROM ALGAL BIOMASS 审中-公开
标题翻译：从ALGAL BIOMASS提取脂肪的方法
公开(公告)号：WO2010089063A1
公开(公告)日：2010-08-12
申请号：PCT/EP2010/000570
申请日：2010-01-28
申请人： ENI S.P.A. , D'ADDARIO, Ezio Nicola , D'ANGELI, Edoardo , MEDICI, Roberto
发明人： D'ADDARIO, Ezio Nicola , D'ANGELI, Edoardo , MEDICI, Roberto
IPC分类号： C11B1/10 , C11C3/00 , C11C3/12 , C12P7/64
CPC分类号： C11B1/10 , C11C3/00 , C11C3/003 , C11C3/12 , Y02E50/13
摘要： A process for the extraction of lipids from algal biomass comprising: - producing an aqueous suspension of algal biomass; - adding to said aqueous suspension of algal biomass at least one organic solvent immiscible or substantially immiscible with water obtaining an organic -aqueous mixture; - subjecting said organic -aqueous mixture to evaporation of water and lipid extraction, operating at a temperature such to obtain the substantial complete removal of the water from said organic -aqueous mixture, obtaining: (i) an organic phase comprising lipids and said organic solvent; (ii) a semi-solid phase comprising a residue of said algal biomass.
摘要翻译：从藻类生物质中提取脂质的方法，包括：产生藻类生物质的水悬浮液; - 向藻类生物质的所述含水悬浮液中加入至少一种与水不混溶或基本上与水不混溶的有机溶剂，得到有机 - 水混合物; - 使所述有机 - 水混合物蒸发水和脂质提取，在如下温度下操作，从而从所述有机 - 水性混合物中实质上完全除去水，得到：（i）包含脂质和所述有机溶剂的有机相 ; （ii）包含所述藻类生物质的残余物的半固相。

3. 发明申请

WO2015075630A1 PROCESS FOR THE EXTRACTION OF LIPIDS AND SUGARS FROM ALGAL BIOMASS 审中-公开
标题翻译：从ALGAL BIOMASS提取脂肪和糖的方法
公开(公告)号：WO2015075630A1
公开(公告)日：2015-05-28
申请号：PCT/IB2014/066130
申请日：2014-11-18
申请人： ENI S.P.A.
发明人： MASSETTI, Felicia , CAPUANO, Federico , MEDICI, Roberto , MIGLIO, Roberta
IPC分类号： C11C1/10 , C12N1/12 , C12P19/00
CPC分类号： C11B1/10 , C12N1/02 , C12N1/12 , C13K1/00 , C13K13/002 , C13K13/007 , Y02E50/13
摘要： Process for the extraction of lipids and sugars from algal biomass, which comprises: - producing an aqueous suspension of algal biomass; - bringing the pH of said aqueous suspension of algal biomass to a value higher than or equal to 10, preferably ranging from 10.2 to 12, obtaining an aqueous suspension of algal biomass at basic pH; - adding at least one anionic flocculant to said aqueous suspension of algal biomass at basic pH obtaining a concentrated algal biomass; - recovering said concentrated algal biomass; -subjecting said concentrated algal biomass to extraction of the lipids obtaining: (i) an organic phase comprising lipids; (ii) a semi-solid phase comprising a residue of said algal biomass; - subjecting said semi-solid phase (ii) to hydrolysis obtaining sugars. The lipids thus obtained can be advantageously used in the production of biodiesel or green diesel which can be used as such, or in a mixture with other fuels for motor vehicles. The sugars thus obtained can be advantageously used as carbon sources in fermentation processes for the production of lipids and also for the production of alcohols (e.g., ethanol, butanol). Said alcohols can be advantageously used as biofuels for motor vehicles or as components that can be added to fuels for motor vehicles.
摘要翻译：从藻类生物质中提取脂质和糖的方法，其包括： - 产生藻类生物质的水悬浮液; - 将藻类生物质的所述水性悬浮液的pH值提高到高于或等于10，优选为10.2至12的值，得到碱性pH下的藻类生物质的水悬浮液; - 在碱性pH下向藻类生物质的所述水性悬浮液中加入至少一种阴离子絮凝剂，得到浓缩藻类生物质; - 回收所述浓缩藻类生物质; - 将所述浓缩的藻类生物质引入所述脂质的提取中，获得：（i）包含脂质的有机相; （ii）包含所述藻类生物质的残余物的半固相; - 对所述半固相（ii）进行水解获得糖。由此获得的脂质可以有利地用于生产生物柴油或绿色柴油，其可以原样使用，或与用于机动车辆的其它燃料的混合物。由此获得的糖可有利地用作用于生产脂质的发酵方法中的碳源，也可用于生产醇（例如乙醇，丁醇）。所述醇可以有利地用作机动车辆的生物燃料或作为机动车辆燃料的组分。

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