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    • 2. 发明申请
    • COMPOSITIONS AND METHODS FOR INCREASING PLANT TOLERANCE TO HIGH POPULATION DENSITY
    • 增加植物对高人口密度的耐受性的组合物和方法
    • WO2007103956A2
    • 2007-09-13
    • PCT/US2007063445
    • 2007-03-07
    • PIONEER HI BRED INTDU PONTBATE NICHOLAS JAUKERMAN MILO J
    • BATE NICHOLAS JAUKERMAN MILO J
    • C12N15/82
    • C12N15/8261C07K14/415Y02A40/146
    • Compositions and methods for suppressing the shade-avoidance response of plants and improving plant yield are provided. Compositions of the invention include an early flowering 3 (ELF3) maize gene, the promoter for this gene, an Arabidopsis basic helix-loop-helix transcription factor (bHLH-041), and fragments and variants thereof. The ELF3 promoter sequence is useful for driving expression of polynucleotides of interest in a plant. The ELF3 and bHLH-041 sequences of the invention, or variants and fragments thereof, are provided in expression cassettes for use in manipulating expression of the ELF3 and bHLH-041 genes. By increasing expression of ELF3 and/or suppressing expression of bHLH-041, the methods of the invention provide for altered response of a plant to light quality and suppression of the high-density-invoked survival mode of development. The invention thus provides methods for growing crop plants at high population densities for yield enhancement. Transformed plants having the altered shade-avoidance phenotype of the invention, and seeds of said plants, are also provided.
    • 提供了抑制植物避光反应和提高植物产量的组合物和方法。 本发明的组合物包括早期开花3(ELF3)玉米基因,该基因的启动子,拟南芥碱性螺旋 - 环 - 螺旋转录因子(bHLH-041)及其片段和变体。 ELF3启动子序列可用于驱动植物中感兴趣的多核苷酸的表达。 在用于操纵ELF3和bHLH-041基因表达的表达盒中提供本发明的ELF3和bHLH-041序列或其变体和片段。 通过增加ELF3的表达和/或抑制bHLH-041的表达,本发明的方法提供植物对光质量的响应改变和抑制高密度诱导的存活发育模式。 因此,本发明提供了用于在高种群密度下种植作物以提高产量的方法。 还提供了具有本发明的遮荫避免表型和所述植物的种子的转化植物。