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1. 发明申请

WO2008133638A3 XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS FOR ETHANOL PRODUCTION 审中-公开
标题翻译：用于乙醇生产的XYLOSE-ZYMOMONAS的XYLITOL合成突变体
公开(公告)号：WO2008133638A3
公开(公告)日：2009-01-29
申请号：PCT/US2007020950
申请日：2007-09-28
申请人： DU PONT , MIDWEST RESEARCH INST , VIITANEN PAUL V , MCCUTCHEN CAROL M , CHOU YAT-CHEN , ZHANG MIN
发明人： VIITANEN PAUL V , MCCUTCHEN CAROL M , CHOU YAT-CHEN , ZHANG MIN
IPC分类号： C12N1/22 , C12P7/06 , C12P7/10
CPC分类号： C12N1/22 , C12P7/10 , Y02E50/16 , Y02E50/17
摘要： A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism.lt also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
摘要翻译：用葡萄糖 - 果糖氧化还原酶基因的遗传修饰工程改造了一种利用木糖的酵母菌菌株，导致GFOR酶活性的降低。工程化的菌株表现出木糖代谢产物的减少，这是木糖代谢的有害副产物。在过程相关条件下，混合糖发酵过程中还会消耗更多的木糖并产生更多的乙醇。

2. 发明申请

WO2008051348A3 ETHANOL PRODUCTION USING XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS 审中-公开
标题翻译：用木糖醇合成突变型木糖醇突变株生产乙醇
公开(公告)号：WO2008051348A3
公开(公告)日：2008-09-12
申请号：PCT/US2007020945
申请日：2007-09-28
申请人： DU PONT , MIDWEST RESEARCH INST , VIITANEN PAUL V , MCCUTCHEN CAROL M , EMPTAGE MARK , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN
发明人： VIITANEN PAUL V , MCCUTCHEN CAROL M , EMPTAGE MARK , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN
IPC分类号： C12P7/06 , C12N1/22 , C12P7/10
CPC分类号： C12P7/065 , Y02E50/16 , Y02E50/17
摘要： Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
摘要翻译：发现使用具有葡萄糖 - 果糖氧化还原酶基因的遗传修饰的利用木糖的发酵单胞菌菌株生产乙醇，由于木糖醇的产生大大降低，木糖醇是发酵期间合成的木糖代谢的有害副产物，所以发现其得到改善。

3. 发明申请

WO2008133638A2 XYLITOL SYNTHESIS MUTANT OF XYLOSE-UTILIZING ZYMOMONAS FOR ETHANOL PRODUCTION 审中-公开
标题翻译：用于乙醇生产的XYLOSE-ZYMOMONAS的XYLITOL合成突变体
公开(公告)号：WO2008133638A2
公开(公告)日：2008-11-06
申请号：PCT/US2007020950
申请日：2007-09-28
申请人： DU PONT , MIDWEST RESEARCH INST , VIITANEN PAUL V , MCCUTCHEN CAROL M , CHOU YAT-CHEN , ZHANG MIN
发明人： VIITANEN PAUL V , MCCUTCHEN CAROL M , CHOU YAT-CHEN , ZHANG MIN
CPC分类号： C12N1/22 , C12P7/10 , Y02E50/16 , Y02E50/17
摘要： A strain of xylose-utilizing Zymomonas was engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism.lt also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.
摘要翻译：用葡萄糖 - 果糖氧化还原酶基因的遗传修饰工程改造了一种利用木糖的酵母菌菌株，导致GFOR酶活性的降低。工程化的菌株表现出木糖代谢产物的减少，这是木糖代谢的有害副产物。在过程相关条件下，混合糖发酵过程中还会消耗更多的木糖并产生更多的乙醇。

4. 发明申请

WO2008051349A3 IMPROVED ETHANOL PRODUCT I ON IN FERMENTATION OF MIXED SUGARS CONTAINING XYLOSE IN THE PRESENCE OF SUGAR ALCOHOLS 审中-公开
标题翻译：改进的乙醇产品I在糖乳液存在下发酵中含有XYLOSE的混合糖
公开(公告)号：WO2008051349A3
公开(公告)日：2008-08-07
申请号：PCT/US2007020946
申请日：2007-09-28
申请人： DU PONT , MIDWEST RESEARCH INST , VIITANEN PAUL V , MCCUTCHEN CAROL M , LI XU , EMPTAGE MARK , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
发明人： VIITANEN PAUL V , MCCUTCHEN CAROL M , LI XU , EMPTAGE MARK , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
IPC分类号： C12N1/00
CPC分类号： C12N1/20 , C12P7/065 , C12R1/01 , Y02E50/16 , Y02E50/17
摘要： Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
摘要翻译：当发现含木糖的运动发酵单胞菌菌株在含有木糖的混合糖的培养基中生长时，如果在培养基中包含山梨糖醇或甘露醇，则发现其具有改善的乙醇生产。在没有生长滞后期的混合糖的浓度以及较高的糖浓度下观察到效果。

5. 发明申请

WO2009120730A3 HIGH EXPRESSION ZYMOMONAS PROMOTERS 审中-公开
标题翻译：高表达ZYMOMONAS PROMOTERS
公开(公告)号：WO2009120730A3
公开(公告)日：2009-12-10
申请号：PCT/US2009038158
申请日：2009-03-25
申请人： DU PONT , ALLIANCE SUSTAINABLE ENERGY , VIITANEN PAUL V , TAO LUAN , ZHANG YUYING , CAIMI PERRY G , MCCUTCHEN CAROL , MCCOLE LAURA , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
发明人： VIITANEN PAUL V , TAO LUAN , ZHANG YUYING , CAIMI PERRY G , MCCUTCHEN CAROL , MCCOLE LAURA , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
IPC分类号： C12N15/09
CPC分类号： C12N15/74
摘要： Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译：鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体，其直接改善可操作地连接的异源核酸的表达水平。这些是用于在发酵单胞菌，酵母属和其他相关细菌中表达嵌合基因的高表达启动子。

6. 发明申请

WO2009120731A3 ZYMOMONAS WITH IMPROVED XYLOSE UTILIZATION 审中-公开
标题翻译：具有改进的木糖利用率的ZYMOMONAS
公开(公告)号：WO2009120731A3
公开(公告)日：2009-11-12
申请号：PCT/US2009038164
申请日：2009-03-25
申请人： DU PONT , ALLIANCE SUSTAINABLE ENERGY , VIITANEN PAUL V , TAO LUAN , ZHANG YUYING , CAIMI PERRY G , MCCUTCHEN CAROL , MCCOLE LAURA , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
发明人： VIITANEN PAUL V , TAO LUAN , ZHANG YUYING , CAIMI PERRY G , MCCUTCHEN CAROL , MCCOLE LAURA , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
IPC分类号： C12N1/20 , C12N9/92 , C12N15/52
CPC分类号： C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要： Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译：通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因突变启动子的嵌合木糖异构酶基因来改造发酵单胞菌菌株。该启动子指导木糖异构酶表达的增加，并且当该菌株另外工程化用于表达木酮糖激酶，转醛醇酶和转酮醇酶时，获得改进的木糖利用。

7. 发明申请

WO2009058938A3 PROCESS FOR THE PRODUCTION OF ETHANOL FROM A MEDIUM COMPRISING XYLOSE, EMPLOYING A RECOMBINANT ZYMOMONAS STRAIN HAVING A REDUCED HIMA EXPRESSION 审中-公开
标题翻译：从包含XYLOSE的中间产生乙醇的方法，使用具有还原性HIMA表达的重组ZYMOMONASAS菌株
公开(公告)号：WO2009058938A3
公开(公告)日：2009-06-18
申请号：PCT/US2008081694
申请日：2008-10-30
申请人： DU PONT , VIITANEN PAUL V , TAO LUAN , KNOKE KYLE , ZHANG YUYING , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
发明人： VIITANEN PAUL V , TAO LUAN , KNOKE KYLE , ZHANG YUYING , CAIMI PERRY G , ZHANG MIN , CHOU YAT-CHEN , FRANDEN MARY ANN
IPC分类号： C12P7/06
CPC分类号： C12P7/065 , Y02E50/17
摘要： Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of thehimA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
摘要翻译：通过筛选发酵单胞菌突变文库，发现himA基因参与乙酸盐对发酵单胞菌性能的抑制作用。当与含有木糖的混合糖培养基，特别是在乙酸存在下培养时，与亲本菌株相比，进一步工程改造以降低himA基因活性的木糖酶发酵单胞菌菌株发现乙醇产量增加。

8. 发明申请

WO2004037973A3 ZYMOMONAS PENTOSE-SUGAR FERMENTATING STRAINS AND USES THEREOF 审中-公开
标题翻译： ZYMOMONAS PENTOSE-SUGAR发酵菌株及其用途
公开(公告)号：WO2004037973A3
公开(公告)日：2004-09-16
申请号：PCT/US0312726
申请日：2003-04-25
申请人： MIDWEST RESEARCH INST , ZHANG MIN , CHOU YAT-CHEN , HOWE WILLIAM , EDDY CHRISTINE , EVANS KENT
发明人： ZHANG MIN , CHOU YAT-CHEN , HOWE WILLIAM , EDDY CHRISTINE , EVANS KENT
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N9/12 , C12N9/92 , C12N15/52 , C12N15/74 , C12N15/90 , C12P7/06 , C12R1/01 , C12N1/20
CPC分类号： C12N9/1022 , C12N9/1205 , C12N9/92 , C12N15/52 , C12N15/74 , C12N15/90 , C12N15/902 , C12P7/06 , C12P7/065 , Y02E50/16 , Y02E50/17
摘要： A Zymomonas integrant posseses the ability to ferment pentose into ethanol. The operon encoding for the pentose-fermenting enzymes are integrated into the Zymomonas genome in a two-integration event of homologous recombination and transposition. Each operon may include more than one pentose-reducing enzyme encoding sequence. The integrant in some embodiments includes enzyme sequences encoding xylose isomerase, xylulokinase, transketolase and transaldolase. The Zymomonas integrants are highly stable, and retain activity for producing the pentose-fermenting enzyme for between 80 to 160 generations. The integrants are also resistant to acetate inhibition, as the integrants demonstrate efficient ethanol production even in the presence of 8 up to 16 grams acetate per liter media.
摘要翻译：发酵单胞菌具有将戊糖发酵成乙醇的能力。编码戊糖发酵酶的操纵子在同源重组和转座的双重整合事件中整合到发酵单胞菌基因组中。每个操纵子可以包括多于一种戊糖还原酶编码序列。在一些实施方案中的综合体包括编码木糖异构酶，木酮糖激酶，转酮醇酶和转醛醇酶的酶序列。发酵单胞菌的整合体是高度稳定的，并且保留在80至160代之间产生戊糖发酵酶的活性。整合剂也耐乙酸盐抑制，因为即使在每升培养基中存在8至16克乙酸盐的情况下，整合体也显示有效的乙醇生产。

9. 发明申请

WO0183786B1 STABLE ZYMOMONAS MOBILIS XYLOSE AND ARABINOSE FERMENTING STRAINS 审中-公开
标题翻译：稳定的ZYMOMONAS MOBILIS XYLOSE和ARABINOSE发酵菌株
公开(公告)号：WO0183786B1
公开(公告)日：2002-07-18
申请号：PCT/US0111334
申请日：2001-04-06
申请人： MIDWEST RESEARCH INST
发明人： ZHANG MIN , CHOU YAT-CHEN
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N9/12 , C12N9/24 , C12N9/92 , C12N15/74 , C12N15/90 , C12P7/06 , C12R1/01 , C12N15/52
CPC分类号： C12N9/1205 , C12N9/1022 , C12N9/92 , C12N15/74 , C12N15/90 , C12N15/902 , C12P7/06 , Y02E50/17
摘要： The present invention briefly includes a transposon for stable insertion of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt , and at least one promoter for expression of the structural genes in the bacterium, a pair of inverted insertion sequences, the operons contained inside the insertion sequences, and a transposase gene located outside of the insertion sequences. A plasmid shuttle vector for transformation of foreign genes into a bacterial genome, comprising at least one operon having structural genes encoding enzymes selected from the group consisting of xylAxylB, araBAD and tal/tkt , at least one promoter for expression of the structural genes in the bacterium, and at least two DNA fragments having homology with a gene in the bacterial genome to be transfomed, is also provided. The transposon and shuttle vectors are useful in constructing significantly different Zymomonas mobilis strains, according to the present invention, which are useful in the conversion of the cellulose derived pentose sugars into fuels and chemicals, using traditional fermentation technology, because they are stable for expression in a non-selection medium.
摘要翻译：本发明简要地包括用于将外来基因稳定插入到细菌基因组中的转座子，其包含至少一个操纵子，所述操纵子具有编码选自由二甲酰基A，细菌中的结构基因，一对倒置插入序列，插入序列内部的操纵子和位于插入序列外部的转座酶基因。一种用于将外来基因转化到细菌基因组中的质粒穿梭载体，其包含至少一个具有编码选自下组的结构基因的操纵子：xylAxylB，araBAD和tal / tkt，至少一种用于表达还提供了细菌中的结构基因和至少两个与待转染细菌基因组中的基因具有同源性的DNA片段。根据本发明，转座子和穿梭载体可用于构建显着不同的运动发酵单胞菌菌株，其可用于使用传统发酵技术将纤维素衍生的戊糖转化为燃料和化学品，因为它们对于在非选择介质。

10. 发明申请

WO0183784A3 METHOD OF SITE-SPECIFIC INSERTION IN ZYMOMONAS MOBILIS 审中-公开
标题翻译： ZYMOMONAS MOBILIS中的特定插入方法
公开(公告)号：WO0183784A3
公开(公告)日：2003-10-02
申请号：PCT/US0111239
申请日：2001-04-06
申请人： MIDWEST RESEARCH INST
发明人： ZHANG MIN , CHOU YAT-CHEN
IPC分类号： C12N15/09 , C12N15/74 , C12N15/90
CPC分类号： C12N15/74 , C12N15/902
摘要： The present invention briefly provides a method of site-specific insertion in Zymomonas, comprising, providing a Zymomonas gene fragment, interrupting a DNA sequence the fragment, and transforming the Zymomonas through homologous recombination with the interrupted fragment.
摘要翻译：本发明简要提供在发酵单胞菌中特异性插入的方法，其包括：提供酵母单胞菌属基因片段，中断DNA序列片段，并通过与中断片段的同源重组转化酵母单胞菌。

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