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1. 发明申请

WO9949047A3 PLANT 1,3-BETA-D-GLUCAN SYNTHASE AND BRITTLE-1 ENCODING SEQUENCES 审中-公开
标题翻译：植物1,3-β-D-葡萄糖合成酶和BRITTLE-1编码序列
公开(公告)号：WO9949047A3
公开(公告)日：2000-03-30
申请号：PCT/US9906583
申请日：1999-03-22
申请人： DU PONT , ALLEN STEPHEN M , HITZ WILLIAM D , LIGHTNER JONATHAN EDWARD , RAFALSKI J ANTONI
发明人： ALLEN STEPHEN M , HITZ WILLIAM D , LIGHTNER JONATHAN EDWARD , RAFALSKI J ANTONI
IPC分类号： C12N9/10 , C12N15/29 , C12N15/54
CPC分类号： C12N9/1051
摘要： This invention relates to isolated nucleic acid fragments encoding a carbohydrate biosynthetic enzyme, selected from plant 1,3-beta-D-glucan synthases or brittle-1 proteins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the carbohydrate biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the carbohydrate biosynthetic enzyme in a transformed host cell.
摘要翻译：本发明涉及编码选自植物1,3-β-D-葡聚糖合酶或脆性-1蛋白质的碳水化合物生物合成酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分碳水化合物生物合成酶的嵌合基因，其中嵌合基因的表达导致转化宿主细胞中碳水化合物生物合成酶水平的改变。

2. 发明申请

WO0005386A3 CHORISMATE BIOSYNTHESIS ENZYMES 审中-公开
公开(公告)号：WO0005386A3
公开(公告)日：2000-04-27
申请号：PCT/US9916352
申请日：1999-07-20
申请人： DU PONT , FAMODU LAYO O , HITZ WILLIAM D , LIGHTNER JONATHAN EDWARD
发明人： FAMODU LAYO O , HITZ WILLIAM D , LIGHTNER JONATHAN EDWARD
IPC分类号： G01N33/50 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/88 , C12N15/09 , C12N15/60 , C12N15/82 , C12Q1/25 , C12Q1/527 , C12Q1/68 , G01N33/15 , C12Q1/02
CPC分类号： C12N15/8254 , C12N9/88 , C12N15/8251
摘要： This invention relates to an isolated nucleic acid fragment encoding a DAHP synthetase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the DAHP synthetase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the DAHP synthetase in a transformed host cell.
摘要翻译：本发明涉及编码DAHP合成酶的分离的核酸片段。本发明还涉及在正义或反义方向上编码全部或部分DAHP合成酶的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中DAHP合成酶水平的改变。

3. 发明申请

WO0006755A3 MODIFICATION OF STARCH BIOSYNTHETIC ENZYME GENE EXPRESSION TO PRODUCE STARCHES IN GRAIN CROPS 审中-公开
标题翻译：淀粉合成酶基因表达载体在淀粉生产中的应用
公开(公告)号：WO0006755A3
公开(公告)日：2000-09-08
申请号：PCT/US9916296
申请日：1999-07-26
申请人： DU PONT , BROGLIE KAREN E , LIGHTNER JONATHAN EDWARD
发明人： BROGLIE KAREN E , LIGHTNER JONATHAN EDWARD
IPC分类号： A01H5/00 , A01H1/00 , C08B30/00 , C08B33/00 , C12N9/10 , C12N15/09 , C12N15/113 , C12N15/29 , C12N15/54 , C12N15/82 , C12N15/11
CPC分类号： C12N15/1137 , C08B30/00 , C12N9/1051 , C12N15/8245
摘要： The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch synthase enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of cereal crops comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch synthase structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination; and (2) transforming cereal crops with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed cereal crops compared to the fine structure of starch derived from cereal crops not possessing said chimeric gene.
摘要翻译：本发明公开了利用cDNA克隆构建用于抑制玉米中淀粉合酶酶活性的有义和反义基因。更具体地说，本发明涉及一种控制来源于谷类作物谷物的淀粉的淀粉细结构的方法，包括：（1）制备包含编码淀粉合酶结构基因或其片段的核酸片段的嵌合基因，所述核酸片段可操作地连接在上游侧以有义或反义方向与编码指导玉米胚乳组织中基因表达的启动子的核酸片段连接，并且在下游侧与编码用于转录终止的合适调控序列的核酸片段可操作连接; 和（2）用所述嵌合基因转化谷类作物，其中所述嵌合基因的表达导致来自所述转化的谷类作物籽粒的淀粉的细微结构与来自不具有所述谷类作物的谷类作物的淀粉的精细结构相比改变嵌合基因。

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