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1. 发明申请

WO2017025648A1 STEVIOL GLYCOSIDE TRANSPORT 审中-公开
标题翻译： STEVIOL GLYCOSIDE运输
公开(公告)号：WO2017025648A1
公开(公告)日：2017-02-16
申请号：PCT/EP2016/069356
申请日：2016-08-15
申请人： DSM IP ASSETS B.V.
发明人： BOER, Viktor, Marius , ZWARTJENS, Priscilla , VAN DEN BERG, Eric
IPC分类号： C07K14/39 , C12P19/56 , C12P15/00 , C12N1/16 , A23L2/60
CPC分类号： C07K14/39 , A23L2/60 , C12P15/00 , C12P19/56
摘要： A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
摘要翻译：能够产生过表达介导甜菊糖苷转运的多肽的甜菊糖苷的重组宿主，该多肽包含SEQ ID NO：29所示的氨基酸序列或与其序列同一性至少约50％的氨基酸序列。能够产生已经在其基因组中修饰的甜菊糖苷的重组宿主，导致介导甜菊糖苷转运的多肽的产生缺陷，并且该多肽包含SEQ ID NO： 29或与其具有至少约50％序列同一性的氨基酸序列。

2. 发明申请

WO2019211230A1 STEVIOL GLYCOSIDE TRANSPORT 审中-公开
公开(公告)号：WO2019211230A1
公开(公告)日：2019-11-07
申请号：PCT/EP2019/060897
申请日：2019-04-29
申请人： DSM IP ASSETS B.V.
发明人： VAN LEEUWEN, Johannes, Gustaaf, Ernst , BOER, Viktor, Marius , ZWARTJENS, Priscilla , VAN VUGT, Jos , DE JONG, René, Marcel
IPC分类号： C07K14/39 , C12P19/56
摘要： The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.

3. 发明申请

WO2017060318A2 KAURENOIC ACID HYDROXYLASES 审中-公开
标题翻译：杀真菌酸羟脯氨酸
公开(公告)号：WO2017060318A2
公开(公告)日：2017-04-13
申请号：PCT/EP2016/073818
申请日：2016-10-05
申请人： DSM IP ASSETS B.V.
发明人： VAN LEEUWEN, Johannes, Gustaaf, Ernst , ZWARTJENS, Priscilla , BOER, Viktor, Marius
IPC分类号： C12N9/02
CPC分类号： C12N9/0073 , C12Y114/13079
摘要： The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
摘要翻译：本发明涉及具有山梨酸13-羟化酶活性的变体多肽，该变体多肽包含当与包含SEQ ID NO：1所示的序列的山梨酸13-羟化酶比对时的氨基酸序列包含至少一个取代相应于氨基酸72,85,108,127,129,141,172,195,196,197,29,226,236,291,302,361或464中任一个的氨基酸残基，所述位置被定义参考SEQ ID NO：1，并且其中与具有山梨酸13-羟化酶活性的参考多肽相比，变体具有一个或多个修饰的性质。本发明的变体多肽可以用于重组宿主中用于生产甜菊醇或甜菊醇糖苷。

4. 发明申请

WO2018104238A1 KAURENOIC ACID HYDROXYLASES 审中-公开
公开(公告)号：WO2018104238A1
公开(公告)日：2018-06-14
申请号：PCT/EP2017/081390
申请日：2017-12-04
申请人： DSM IP ASSETS B.V.
发明人： VAN LEEUWEN, Johannes, Gustaaf, Ernst , ZWARTJENS, Priscilla , BOER, Viktor, Marius
IPC分类号： C12N9/02 , A23L27/30
摘要： The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

5. 发明申请

WO2016146711A1 UDP-GLYCOSYLTRANSFERASES 审中-公开
标题翻译： UDP糖基转移酶
公开(公告)号：WO2016146711A1
公开(公告)日：2016-09-22
申请号：PCT/EP2016/055734
申请日：2016-03-16
申请人： DSM IP ASSETS B.V.
发明人： BOER, Viktor, Marius , VAN LEEUWEN, Johannes, Gustaaf, Ernst , ZWARTJENS, Priscilla , KOLEN, Catharina, Petronella, Antonia, Maria
IPC分类号： C12N9/10 , C12P19/56
CPC分类号： C12N9/1048 , A23L2/60 , A23L27/36 , C12N9/1051 , C12P5/007 , C12P19/56 , C12Y204/01017
摘要： The present invention relates to polypeptides having UDP- Glycosyltransferase activity, in particular UGT2 activity, and having at least 85% identity to the amino acid sequence set forth in one of SEQ ID NO: 1, 3, 6, 9, 11, 14, 17, 20, 22, 25. The application also relates to recombinant host comprising a recombinant nucleic acid sequence encoding said polypeptides and uses thereof to prepare glycosylated diterpenes, like steviol glycoside. The host cells may comprise further enzymes of the steviol gycoside biosynthesis patway.
摘要翻译：本发明涉及具有UDP-糖基转移酶活性的多肽，特别是UGT2活性，并且与SEQ ID NO：1,3,6,9,11,14，本申请还涉及包含编码所述多肽的重组核酸序列的重组宿主及其用于制备糖基化二萜（如甜菊糖苷）的重组宿主。宿主细胞可以包含甜菊糖苷生物合成培养基的其它酶。

6. 发明申请

WO2018078014A1 GERANYLGERANYL PYROPHOSPHATE SYNTHASES 审中-公开
标题翻译： GERANYLERERANLPYROPHOSPHATE合成
公开(公告)号：WO2018078014A1
公开(公告)日：2018-05-03
申请号：PCT/EP2017/077439
申请日：2017-10-26
申请人： DSM IP ASSETS B.V.
发明人： BOER, Viktor, Marius , ZWARTJENS, Priscilla , VAN LEEUWEN, Johannes, Gustaaf, Ernst
IPC分类号： C12N9/10 , C12P19/44 , C12N15/52
摘要： The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
摘要翻译：本发明涉及具有香叶基香叶基焦磷酸合酶活性的变体多肽，所述变体多肽包含氨基酸序列，所述氨基酸序列当与包含SEQ ID NO：1所示序列的香叶基香叶基焦磷酸合酶比对时，包含对应于位置92,100或235处的任何氨基酸的氨基酸残基的至少一个取代，所述位置参考SEQ ID NO：1进行限定，并且其中所述变体与参照物相比具有一个或多个修饰的性质具有香叶基香叶基焦磷酸合酶活性的多肽。本发明的变体多肽可以在重组宿主中用于生产甜菊醇或甜菊醇糖苷。

7. 发明申请

WO2017025649A1 STEVIOL GLYCOSIDE TRANSPORT 审中-公开
标题翻译： STEVIOL GLYCOSIDE运输
公开(公告)号：WO2017025649A1
公开(公告)日：2017-02-16
申请号：PCT/EP2016/069357
申请日：2016-08-15
申请人： DSM IP ASSETS B.V.
发明人： BOER, Viktor, Marius , ZWARTJENS, Priscilla , VAN DEN BERG, Eric
IPC分类号： C12N1/15 , C12N1/19 , C12N1/21 , C12P19/56 , C07K14/39
CPC分类号： C07K14/39 , C12P19/56
摘要： A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.
摘要翻译：能够产生过表达介导甜菊糖苷转运的多肽的甜菊糖苷的重组宿主，该多肽包含SEQ ID NO：35或SEQ ID NO：38所示的氨基酸序列或具有至少约50个氨基酸序列％序列同一性。能够产生已经在其基因组中修饰的甜菊糖苷的重组宿主，导致介导甜菊糖苷转运的多肽的产生缺陷，并且该多肽包含SEQ ID NO： 35或SEQ ID NO：38或与其之间具有至少约50％序列同一性的氨基酸序列。

8. 发明申请

WO2013110673A1 DITERPENE PRODUCTION 审中-公开
标题翻译：地面生产
公开(公告)号：WO2013110673A1
公开(公告)日：2013-08-01
申请号：PCT/EP2013/051262
申请日：2013-01-23
申请人： DSM IP ASSETS B.V.
发明人： LEHMANN, Martin , TRUEHEART, Joshua , ZWARTJENS, Priscilla , WU, Liang , BOER, Viktor , SAGT, Cornelis, Maria, Jacobus , KUMAR, Manoj , MEIJRINK, Bernard
IPC分类号： A23L1/236 , C12N9/02 , C12N9/88 , C12N9/90 , C12P5/00
CPC分类号： C12P19/56 , A23K20/10 , A23K20/121 , A23K20/163 , A23L2/60 , A23L27/36 , A23V2002/00 , C07H15/256 , C12N9/0073 , C12N9/1051 , C12N9/88 , C12N9/90 , C12N15/52 , C12P5/007 , C12Y114/13078 , C12Y204/01035 , C12Y402/03019 , C12Y505/01013
摘要： The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.
摘要翻译：本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。

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