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1. 发明申请

WO2010142960A1 SIGNAL AMPLIFICATION MICROSPHERES, THEIR USE IN ONE-STEP AND MULTI-STEP ANALYTICAL AMPLIFICATION PROCEDURES AND METHODS FOR THEIR PRODUCTION 审中-公开
标题翻译：信号放大微球，它们在一步和多步分析放大过程中的应用及其生产方法
公开(公告)号：WO2010142960A1
公开(公告)日：2010-12-16
申请号：PCT/GB2010/001144
申请日：2010-06-10
申请人： DNA SUPERNOVA LTD , BALL, Christopher , MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard
发明人： MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard
IPC分类号： G01N33/52 , G01N33/543 , G01N33/566 , G01N33/68 , B01J13/02
CPC分类号： G01N33/54313
摘要： The present invention relates to microspheres comprising protein signal precursor molecules, or a carrier protein bonded to signal precursor molecules, wherein said signal precursor molecules are activatable to generate a detectable signal whilst remaining bonded to the carrier protein. Also disclosed is a method of making such microspheres comprising the steps of mixing protein molecules with a matrix former in solution; adding a reducing reagent to the mixture; removing the reducing reagent; and removing the matrix former to leave microspheres of protein molecules. Also disclosed are bioassay methods using the microspheres to provide signal amplification, including an amplification cycling procedure.
摘要翻译：本发明涉及包含蛋白质信号前体分子或与信号前体分子结合的载体蛋白的微球，其中所述信号前体分子可激活以产生可检测信号，同时保持与载体蛋白结合。还公开了制备这种微球的方法，其包括将蛋白质分子与溶液中的基质原料混合的步骤; 向混合物中加入还原剂; 去除还原剂; 并去除基质原子以留下蛋白质分子的微球。还公开了使用微球体提供信号放大的生物测定方法，包括扩增循环程序。

2. 发明申请

WO2010142963A1 METHODS OF SIGNAL GENERATION AND SIGNAL LOCALIZATION FOR IMPROVEMENT OF SIGNAL READABILITY IN SOLID PHASE BASED BIOASSAYS 审中-公开
标题翻译：信号生成和信号定位方法，用于提高基于固体相位生物信息学的信号可读性
公开(公告)号：WO2010142963A1
公开(公告)日：2010-12-16
申请号：PCT/GB2010/001152
申请日：2010-06-14
申请人： DNA SUPERNOVA LTD , BALL, Christopher , MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard , SIN, King Keung
发明人： MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard , SIN, King Keung
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , B01L3/5055 , B01L2300/043 , B01L2300/046 , B01L2300/069 , B01L2300/0816 , B01L2300/0825 , G01N33/53 , G01N33/5302 , G01N33/537 , G01N33/558
摘要： The invention provides a method for generating and localizing a signal in a solid phase substrate detection system, comprising: applying a solution of a target material to a solid phase substrate; binding said target material with a specific affinity molecule for that target material to which a non-catalytic label is attached, said non-catalytic label comprising a plurality of signal precursor molecules; applying a carrier medium to said solid phase substrate, and treating the label to convert the plurality of signal precursor molecules to a plurality of detectable signal generating molecules; wherein said carrier medium comprises a solvent for the dissolution of the non-catalytic label and a thickener for causing localization of the signal generated by said plurality of detectable signal generating molecules indicating the presence and/or quantity of said target. In some embodiments, the carrier medium may also include a signal developing reagent that converts the plurality of signal precursor molecules to a plurality of detectable signal generating molecules. In other embodiments, a signal developing reagent is not necessary because the plurality of signal precursor molecules is converted to a plurality of detectable signal generating molecules by physical means such as change in temperature, change in pH, sonication, light irradiation or microwave heating. A test device for detecting a target in a fluid sample, and a kit of parts for determining the presence of a target in a fluid sample are also disclosed.
摘要翻译：本发明提供了一种用于在固相衬底检测系统中产生和定位信号的方法，包括：将目标材料的溶液施加到固相衬底; 将所述靶材料与所述非催化标记所连接的靶材料的特异性亲和分子结合，所述非催化标记包含多个信号前体分子; 将载体介质施加到所述固相衬底，以及处理所述标签以将所述多个信号前体分子转化为多个可检测信号产生分子; 其中所述载体介质包含用于溶解所述非催化标记的溶剂和用于引起所述多个可检测信号产生分子产生的信号的定位的增稠剂，其指示所述靶的存在和/或数量。在一些实施方案中，载体介质还可以包括将多个信号前体分子转化为多个可检测信号产生分子的信号显影试剂。在其它实施方案中，信号显影试剂不是必需的，因为多个信号前体分子通过诸如温度变化，pH变化，超声处理，光照射或微波加热的物理手段转化成多个可检测信号产生分子。还公开了一种用于检测流体样品中的靶的测试装置和用于确定流体样品中靶的存在的一组试剂盒。

3. 发明申请

WO2003078659A3 MICROCAPSULES WITH CONTROLABLE PERMEABILITY ENCAPSULATING A NUCLEIC ACID AMPLIFICATION REACTION MIXTURE AND THEIR USE AS REACTION COMPARTMENTS FOR PARALLELS REACTIONS 审中-公开
公开(公告)号：WO2003078659A3
公开(公告)日：2003-09-25
申请号：PCT/EP2003/002926
申请日：2003-03-20
申请人： INNOVATIVEBIO.BIZ , TRAU, Dieter , RENNEBERG, Reinhard , MAK, Wing, Cheung
发明人： TRAU, Dieter , RENNEBERG, Reinhard , MAK, Wing, Cheung
IPC分类号： C12Q1/68
摘要： The present invention refers to microcapsules with high permeability for low molecular weight molecules and no permeability for high molecular weight molecules encapsulating a nucleic acid amplification reaction mixture, in particular, a PCR reaction mixture and providing a compartment to perform a nucleic acid amplification, in particular, a PCR reaction and to applications of such capsules to perform parallel PCR, screening, construction of DNA libraries and sequencing.

4. 发明申请

WO2003078659A2 MICROCAPSULES ENCAPSULATING A NUCLEIC ACID AMPLIFICATION REACTION MIXTURE AND THEIR USE AS REACTION COMPARTMENTS FOR PARALLEL REACTIONS 审中-公开
标题翻译：包裹核酸扩增反应混合物的微胶囊及其作为用于平行反应的反应室的用途
公开(公告)号：WO2003078659A2
公开(公告)日：2003-09-25
申请号：PCT/EP2003/002926
申请日：2003-03-20
申请人： INNOVATIVEBIO.BIZ , TRAU, Dieter , RENNEBERG, Reinhard , MAK, Wing, Cheung
发明人： TRAU, Dieter , RENNEBERG, Reinhard , MAK, Wing, Cheung
IPC分类号： C12Q1/68
CPC分类号： C12N15/1075 , B01J13/02 , B01J13/14 , B82Y10/00 , B82Y30/00 , C12Q1/6844 , C12Q1/6869
摘要： The present invention refers to microcapsules with high permeability for low molecular weight molecules and no permeability for high molecular weight molecules encapsulating a nucleic acid amplification reaction mixture, in particular, a PCR reaction mixture and providing a compartment to perform a nucleic acid amplification, in particular, a PCR reaction and to applications of such capsules to perform parallel PCR, screening, construction of DNA libraries and sequencing.
摘要翻译：本发明涉及低分子量分子具有高渗透性并且对于包封核酸扩增反应混合物，特别是PCR反应混合物的高分子量分子不具有渗透性的微胶囊，特别是PCR反应混合物，进行核酸扩增，特别是PCR反应，并进行这种胶囊的应用以进行平行PCR，筛选，构建DNA文库和测序。

5. 发明申请

WO2009132510A1 LIPOCALIN-2 AS A PROGNOSTIC AND DIAGNOSTIC MARKER FOR HEART AND STROKE RISKS 审中-公开
标题翻译： LIPOCALIN-2作为心血管和危险因素的预防和诊断标记
公开(公告)号：WO2009132510A1
公开(公告)日：2009-11-05
申请号：PCT/CN2009/000405
申请日：2009-04-16
申请人： VERSITECH LIMITED , R & C BIOGENIUS LIMITED , XU, Aimin , WANG, Yu , RENNEBERG, Reinhard , CAUTHERLEY, George William Hunter , CHAN, Puiyee, Cangel , LEHMANN, Matthias
发明人： XU, Aimin , WANG, Yu , RENNEBERG, Reinhard , CAUTHERLEY, George William Hunter , CHAN, Puiyee, Cangel , LEHMANN, Matthias
IPC分类号： A61K38/43 , G01N33/53 , C07K14/47 , A61P9/00
CPC分类号： C07K14/47 , A61K38/00 , C07K16/18 , G01N33/6872 , G01N2800/32 , Y10S436/811
摘要： Methods and apparatus are disclosed for the measurement of lipocalin-2 in body fluid (including but not limited to blood, serum, plasma, urine, saliva, tear, etc) by an assay such as a immunoassay or an immunotest for (1) the prediction of risk of future cardiovascular diseases and (2) the determination of the likelihood that certain individuals will benefit to a greater o lesser extent from the use of certain treatments designed to prevent and/or treat cardiovascula diseases.
摘要翻译：公开了通过诸如免疫测定法或免疫测定法的体液（包括但不限于血液，血清，血浆，尿液，唾液，泪液等）中脂质运载蛋白-2的测量的方法和装置（1）预测未来心血管疾病的风险和（2）确定某些个体在使用旨在预防和/或治疗心血管疾病的某些治疗方面可能受益较大的可能性。

6. 发明申请

WO2007128286A1 VERFAHREN ZUM GEKOPPELTEN ENZYM-IMMUNCHEMISCHEN NACHWEIS VON ANALYTEN MITTELS ENDOGENER KALIBRATOREN 审中-公开
标题翻译：方法偶联的酶免疫化学检测分析物植物内源校准器
公开(公告)号：WO2007128286A1
公开(公告)日：2007-11-15
申请号：PCT/DE2007/000824
申请日：2007-05-07
申请人： 8SENS.BIOGNOSTIC GMBH , RENNEBERG, Reinhard , CAUTHERLEY, George, W., H. , CHAN, Cangel, P., Y. , LEHMANN, Matthias , LEHMANN, Karin
发明人： RENNEBERG, Reinhard , CAUTHERLEY, George, W., H. , CHAN, Cangel, P., Y. , LEHMANN, Matthias , LEHMANN, Karin
IPC分类号： G01N33/543
CPC分类号： C12Q1/25 , C12Q1/54
摘要： Die Erfindung betrifft ein Verfahren zum Nachweis von zahlreichen Analyten, wie z.B. von Metaboliten und Antigenen in biologischen oder anderen flüssigen Proben, mittels Analyseelementen, insbesondere lateral-flow Teststreifen, Durchfluss- Membransystemen (flow-through Test), Wells/Kavitäten von Mikrotiterplatten oder Röhrchen, wobei das Verfahren auf miteinander gekoppelte Enzym- und Affinitätsreaktionen beruht und mittels endogener Kalibratoren, also endogen gebildeter Substanzen, mit deren Hilfe Verdünnungen von Probenmatrices korrigiert werden können (z.B. Kreatinin, Glucose, Glucose-6-Phosphat, Lactat, Glutamat, Aspartat, Cholesterol, Pyruvat, Harnstoff und Triglyceride), realisiert wird. Anwendungsgebiete der Erfindung sind vor allem die medizinische Diagnostik, die pharmazeutische Industrie und der Umweltschutz. Bevorzugt betrifft die Erfindung den simultanen oder sequentiellen Nachweis von Antigenen und Metaboliten. Dabei handelt es sich im Sinne der Erfindung vor allem um hochmolekulare Antigene, wie z.B. Proteine, oder niedermolekulare Haptene, wie z.B. Pestizide, Neopterin, Schadstoffe oder Hormone, bei den Metaboliten z.B. um Glucose oder Kreatinin. Die Ergebnisse können mit Hilfe eines Nomogramms, Komparators (Referenzstreifen), Lesegerätes oder durch den bloßen visuellen Vergleich direkt aus dem Assay ermittelt werden.
摘要翻译：本发明涉及一种用于检测分析物众多，如生物或其他液体检验样品中的代谢物和抗原的，由分析元件，尤其是横流测试条的装置，流的膜系统（流动测定），井的微量滴定板或试管的/井，该方法是基于相互耦合酶和亲和反应，且指内源性校准器，如此形成的内源性物质可以与样品基体（例如，肌酸酐，葡萄糖，葡萄糖-6-磷酸，乳酸盐，谷氨酸盐，天冬氨酸盐，胆甾醇，丙酮酸，尿素和甘油三酸酯）的稀释液的辅助下被校正，实现。本发明的应用是特别医疗诊断，医药行业和环境保护。优选地，本发明涉及抗原和代谢物的同时或依次检测。这是根据本发明的，尤其是高分子量的抗原，例如蛋白质或低分子半抗原，如杀虫剂，新蝶呤，污染物或激素，例如在代谢物为葡萄糖或肌酐。结果可以与列线图的辅助下，比较器（参考条），通过从测定中仅仅视觉直接比较来确定所述读取装置或。

7. 发明申请

WO2003044531A3 FUNKTIONALISIERTE TRÄGERKARTE UND DEREN VERWENDUNG ZUR SCHNELLEN DIAGNOSE EINES INFARKTES AUF DER BASIS EINES IMMUNOTESTS 审中-公开
公开(公告)号：WO2003044531A3
公开(公告)日：2003-05-30
申请号：PCT/EP2002/013023
申请日：2002-11-20
申请人： RENNESENS GMBH , RENNEBERG, Reinhard , LEHMANN, Matthias , ZIEGLER, Jürgen
发明人： RENNEBERG, Reinhard , LEHMANN, Matthias , ZIEGLER, Jürgen
IPC分类号： G01N33/543
摘要： Die Erfindung betrifft eine funktionalisierte Trägerkarte und deren Verwendung zur schnellen Diagnose eines Infarktes auf der Basis eines Immunotests zum Nachweis von (Human-heart) Fettsäure-Bindungsprotein (h-FABP) zur personenbezogenen Erfassung, Speicherung und Auswertung von biologischen Flüssigkeiten, insbesondere von Blutproben.

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