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1. 发明申请

WO2010142963A1 METHODS OF SIGNAL GENERATION AND SIGNAL LOCALIZATION FOR IMPROVEMENT OF SIGNAL READABILITY IN SOLID PHASE BASED BIOASSAYS 审中-公开
标题翻译：信号生成和信号定位方法，用于提高基于固体相位生物信息学的信号可读性
公开(公告)号：WO2010142963A1
公开(公告)日：2010-12-16
申请号：PCT/GB2010/001152
申请日：2010-06-14
申请人： DNA SUPERNOVA LTD , BALL, Christopher , MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard , SIN, King Keung
发明人： MAK, Wing Cheung , WONG, Ling Wai , CHAN, Pui Yee Cangel , RENNEBERG, Reinhard , SIN, King Keung
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , B01L3/5055 , B01L2300/043 , B01L2300/046 , B01L2300/069 , B01L2300/0816 , B01L2300/0825 , G01N33/53 , G01N33/5302 , G01N33/537 , G01N33/558
摘要： The invention provides a method for generating and localizing a signal in a solid phase substrate detection system, comprising: applying a solution of a target material to a solid phase substrate; binding said target material with a specific affinity molecule for that target material to which a non-catalytic label is attached, said non-catalytic label comprising a plurality of signal precursor molecules; applying a carrier medium to said solid phase substrate, and treating the label to convert the plurality of signal precursor molecules to a plurality of detectable signal generating molecules; wherein said carrier medium comprises a solvent for the dissolution of the non-catalytic label and a thickener for causing localization of the signal generated by said plurality of detectable signal generating molecules indicating the presence and/or quantity of said target. In some embodiments, the carrier medium may also include a signal developing reagent that converts the plurality of signal precursor molecules to a plurality of detectable signal generating molecules. In other embodiments, a signal developing reagent is not necessary because the plurality of signal precursor molecules is converted to a plurality of detectable signal generating molecules by physical means such as change in temperature, change in pH, sonication, light irradiation or microwave heating. A test device for detecting a target in a fluid sample, and a kit of parts for determining the presence of a target in a fluid sample are also disclosed.
摘要翻译：本发明提供了一种用于在固相衬底检测系统中产生和定位信号的方法，包括：将目标材料的溶液施加到固相衬底; 将所述靶材料与所述非催化标记所连接的靶材料的特异性亲和分子结合，所述非催化标记包含多个信号前体分子; 将载体介质施加到所述固相衬底，以及处理所述标签以将所述多个信号前体分子转化为多个可检测信号产生分子; 其中所述载体介质包含用于溶解所述非催化标记的溶剂和用于引起所述多个可检测信号产生分子产生的信号的定位的增稠剂，其指示所述靶的存在和/或数量。在一些实施方案中，载体介质还可以包括将多个信号前体分子转化为多个可检测信号产生分子的信号显影试剂。在其它实施方案中，信号显影试剂不是必需的，因为多个信号前体分子通过诸如温度变化，pH变化，超声处理，光照射或微波加热的物理手段转化成多个可检测信号产生分子。还公开了一种用于检测流体样品中的靶的测试装置和用于确定流体样品中靶的存在的一组试剂盒。

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