专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2003042412A1 RECA-NEGATIVE AND RHAB-NEGATIVE MICROORGANISM 审中-公开
标题翻译： RECA阴性和RHAB阴性微生物
公开(公告)号：WO2003042412A1
公开(公告)日：2003-05-22
申请号：PCT/EP2002/011979
申请日：2002-11-11
申请人： DEGUSSA AG , MAY, Oliver , LIEBETON, Klaus , ECK, Jürgen
发明人： MAY, Oliver , LIEBETON, Klaus , ECK, Jürgen
IPC分类号： C12R1/19
CPC分类号： C12R1/19 , C12N15/68 , C12N15/70 , C12P13/04 , C12P21/02 , C12P41/006
摘要： The present invention describes new microorganisms specifically for use as expression systems for rhamnose-inducible promoters. Due to the combination of rhamnose and recombination deficiency artificially created in these organisms, expression systems which require less of the expensive rhamnose for induction and therefore can be induced more inexpensively, and have a higher genetic stability are obtained.
摘要翻译：本发明描述了专门用作鼠李糖诱导型启动子表达系统的新微生物。由于在这些生物体中人造产生的鼠李糖和重组缺陷的组合，需要较少昂贵的鼠李糖用于诱导并因此可以更廉价地诱导并具有较高遗传稳定性的表达系统。

2. 发明申请

WO2005090595A2 NITRILE HYDRATASES FROM METAGENOME LIBRARIES 审中-公开
标题翻译：来自METAGENOME图书馆的NITRILE HYDRATASES
公开(公告)号：WO2005090595A2
公开(公告)日：2005-09-29
申请号：PCT/EP2005/002556
申请日：2005-03-10
申请人： DEGUSSA AG , VERSECK, Stefan , LIEBETON, Klaus , ECK, Jürgen
发明人： VERSECK, Stefan , LIEBETON, Klaus , ECK, Jürgen
IPC分类号： C12Q1/68
CPC分类号： C12N9/88 , C12Y402/01084
摘要： The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.
摘要翻译：本发明涉及新型腈水合酶的制备。这些后者优选通过基于PCR的筛选从使用特殊简并引物的宏基因组DNA文库中获得来自非可培养生物体。

3. 发明申请

WO2005090577A1 EXPRESSION OF NITRILLE HYDRATASES IN A TWO-VECTOR EXPRESSION SYSTEM 审中-公开
标题翻译：在两个向量表达系统中表达NITRILLE HYDRATASES
公开(公告)号：WO2005090577A1
公开(公告)日：2005-09-29
申请号：PCT/EP2005/001688
申请日：2005-02-18
申请人： DEGUSSA AG , VERSECK, Stefan , OSSWALD, Steffen , PHONG, Wai-Yee , LIEBETON, Klaus , ECK, Jürgen
发明人： VERSECK, Stefan , OSSWALD, Steffen , PHONG, Wai-Yee , LIEBETON, Klaus , ECK, Jürgen
IPC分类号： C12N15/52
CPC分类号： C12N15/70 , C12N9/88
摘要： The present invention relates to a novel system for expressing nitrile hydratase. In this system, the nitrile hydratases, which are composed of subunits, are formed such that the respective subunits are located on different plasmids and are expressed simultaneously in E. coli.
摘要翻译：本发明涉及一种表达腈水合酶的新型体系。在该系统中，形成由亚基组成的腈水合酶，使得各个亚基位于不同的质粒上并在大肠杆菌中同时表达。

4. 发明申请

WO2005103239A2 NOVEL ALCOHOL DEHYDROGENASES 审中-公开
标题翻译：新型酒精脱水剂
公开(公告)号：WO2005103239A2
公开(公告)日：2005-11-03
申请号：PCT/EP2005/002557
申请日：2005-03-10
申请人： DEGUSSA AG , SCHULZE, Renate , LORENZ, Patrick , ECK, Jürgen , MAY, Oliver , GRÖGER, Harald , TRAUTHWEIN, Harald
发明人： SCHULZE, Renate , LORENZ, Patrick , ECK, Jürgen , MAY, Oliver , GRÖGER, Harald , TRAUTHWEIN, Harald
IPC分类号： C12N9/04
CPC分类号： C12P7/02 , C12N9/0006 , C12Y101/01002
摘要： The invention relates to novel polypeptides which have the biological activity of an NAD- or NADP-dependent alcohol dehydrogenase. The invention furthermore relates to nucleic acids encoding said polypeptides, to non-human hosts or host cells and to reaction systems which may be used for preparing desired products. The polypeptides of the invention are preferably used in the preparation, starting from aldehydes or ketones, of primary and enantiomerically pure secondary alcohol’s which may serve as intermediates for medicaments. Alternatively, the polypeptides of the invention may also be employed in the reverse reaction, i.e. the oxidation of alcohol’s with the formation of aldehydes or ketones.
摘要翻译：本发明涉及具有NAD-或NADP依赖性醇脱氢酶的生物活性的新型多肽。本发明还涉及编码所述多肽，非人宿主或宿主细胞的核酸和可用于制备所需产物的反应体系。本发明的多肽优选用于从醛或酮开始制备可以作为药物中间体的初级和对映异构纯的仲醇。或者，本发明的多肽也可以用于反向反应，即醇与醛或酮的形成的氧化。

5. 发明申请

WO2007128496A2 A NOVEL GROUP OF ESTERASES FOR THE ENANTIOSELECTIVE PRODUCTION OF FINE AND SPECIALITY CHEMICALS 审中-公开
标题翻译：一种新的精选和特种化学品生产的新一代产品
公开(公告)号：WO2007128496A2
公开(公告)日：2007-11-15
申请号：PCT/EP2007/003919
申请日：2007-05-03
申请人： B.R.A.I.N. AG , LIEBETON, Klaus , ECK, Jürgen , BORNSCHEUER, Uwe , BÖTTCHER, Dominique , LANGER, Peter , BELLUR, Esen
发明人： LIEBETON, Klaus , ECK, Jürgen , BORNSCHEUER, Uwe , BÖTTCHER, Dominique , LANGER, Peter , BELLUR, Esen
IPC分类号： C12N9/18 , C12N15/55 , C07K16/40 , C12N5/10
CPC分类号： C12N9/18
摘要： The present invention relates to a polynucleotide encoding an enzyme having carboxylesterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5, 7, 9 and 11; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxylesterase activity which polynucleotide is at least 66% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6, 8, 10 and 12; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a process for producing a polypeptide having carboxylesterase [E. C. 3.1.1.1] activity, comprising culturing the host of the present invention and recovering the polypeptide produced by said host. Moreover, The present invention also relates to a process for producing bacteria or eukaryotic cells capable of expressing a polypeptide having carboxylesterase [E. C. 3.1.1.1] activity, the process comprising genetically engineering bacteria or eukaryotic cells with the vector of the present invention. Finally, the present invention relates to (poly)peptides, antibodies, compositions and various methods for the production of optically active compounds.
摘要翻译：本发明涉及编码具有羧酸酯酶[E. 3.1.1.1]活性，其中编码序列选自（a）编码SEQ ID NO：2,4,6,8,10和12中任一个所示的氨基酸序列的多核苷酸 ; （b）具有或包含编码酶的核苷酸序列的多核苷酸，其中所述核酸序列如SEQ ID NO：1,3,5,7,9和11中任一个所示; （c）具有或包含编码由（a）或（b）的多核苷酸编码的酶的片段或衍生物的核苷酸序列的多核苷酸，其中在所述衍生物中，与氨基酸相比，一个或多个氨基酸残基被保守取代（a）的顺序; （d）编码具有羧酸酯酶活性的多核苷酸的多核苷酸与编码如SEQ ID NO：2,4,6,8,10和12之一所示的酶的多核苷酸至少66％相同; （e）具有或包含核苷酸序列的多核苷酸，其互补链与（a）至（d）中任一项所定义的多核苷酸杂交; 和（T）具有或包含与（d）或（e）的多核苷酸的核苷酸序列退化的核苷酸序列的多核苷酸; 或（a）至（f）的这种多核苷酸或其片段的互补链用作特异性探针或引物。本发明还涉及用本发明的多核苷酸或本发明的载体进行遗传工程改造的宿主。本发明还涉及产生具有羧酸酯酶[E. 3.1.1.1]活性，包括培养本发明的宿主并回收由所述宿主产生的多肽。此外，本发明还涉及能够表达具有羧酸酯酶[E]的多肽的细菌或真核细胞的生产方法。 3.1.1.1]活性，该方法包括用本发明的载体将细菌或真核细胞遗传工程化。最后，本发明涉及（多）肽，抗体，组合物和用于制备光学活性化合物的各种方法。

6. 发明申请

WO2007115797A3 A GROUP OF NOVEL ENANTIOSELECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY 审中-公开
公开(公告)号：WO2007115797A3
公开(公告)日：2007-10-18
申请号：PCT/EP2007/003114
申请日：2007-04-05
申请人： B.R.A.I.N. AG , ECK, Jürgen , LIEBETON, Klaus , FISCHER, Lutz , LUTZ-WAHL, Sabine , HENSEL, Martina , EWERT, Christian , WÄLZ, Marcus
发明人： ECK, Jürgen , LIEBETON, Klaus , FISCHER, Lutz , LUTZ-WAHL, Sabine , HENSEL, Martina , EWERT, Christian , WÄLZ, Marcus
IPC分类号： C12N9/14 , C12N9/78 , C12N1/20 , C12P13/02 , C12P41/00 , C07K14/195
摘要： The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E. C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.

7. 发明申请

WO2003054544A2 VERFAHREN ZUR BESTIMMUNG DER RESPONSIVITÄT EINES INDIVIDUUMS FÜR MISTELLEKTIN 审中-公开
标题翻译：方法用于确定个体的响应到槲寄生凝集素
公开(公告)号：WO2003054544A2
公开(公告)日：2003-07-03
申请号：PCT/EP2002/014682
申请日：2002-12-20
申请人： VISCUM AG , MÜTHING, Johannes , PETER-KATALINIC, Jasna , LANGER, Martin , MÖCKEL, Babette , ECK, Jürgen
发明人： MÜTHING, Johannes , PETER-KATALINIC, Jasna , LANGER, Martin , MÖCKEL, Babette , ECK, Jürgen
IPC分类号： G01N33/566
CPC分类号： C12Q1/48 , C07K16/18 , G01N33/566 , G01N33/66 , G01N2333/42 , G01N2400/00 , G01N2800/52
摘要： Beschrieben wird ein Verfahren zur Bestimmung der Responsivität eines Individuums auf Mistellektin oder für (eine) Mistellektin-Einzellkette(n), wobei die Expression eines membranständigen Rezeptors für Mistellektin charakteristisch für eine entsprechende Responsivität ist.
摘要翻译：公开的是用于确定槲寄生凝集素或的（a）外源凝集素槲寄生Einzellkette（n），其中对于槲寄生凝集素膜结合受体的表达是一个相应的响应性的特性的个体的响应性的方法。

8. 发明申请

WO2003054177A2 NEUE GLYKOSYLHYDROLASEN 审中-公开
标题翻译：新糖
公开(公告)号：WO2003054177A2
公开(公告)日：2003-07-03
申请号：PCT/EP2002/014210
申请日：2002-12-13
申请人： HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN , BREVES, Roland , MAURER, Karl-Heinz , ECK, Jürgen , LORENZ, Patrick , ZINKE, Holger
发明人： BREVES, Roland , MAURER, Karl-Heinz , ECK, Jürgen , LORENZ, Patrick , ZINKE, Holger
IPC分类号： C12N9/00
CPC分类号： C12N9/2414 , C07K2319/00 , C11D3/386 , Y02E50/17
摘要： Die vorliegende Erfindung betrifft neue Glykosylhydrolasen mit amylolytischer Aktivität und die für diese Glykosylhydrolasen codierenden Nukleinsäuren, ein PCR-basiertes Verfahren zur Identifizierung und Gewinnung neuer Glykosylhydrolasen aus Metagenom-DNA sowie diverse technische Anwendungsmöglichkeiten für derartige Glykosylhydrolasen mit amylolytischer Aktivität. Hierunter sind Wasch- und Reinigungsmittel mit solchen Enzymen sowie entsprechende Verfahren und Verwendungsmöglichkeiten von besonderem Interesse.
摘要翻译：本发明涉及具有淀粉分解活性的新的糖基水解酶和编码这些糖基水解酶，用于鉴定和获得来自宏基因组DNA，以及用于与淀粉分解活性，例如糖基水解酶的各种技术应用的新糖基水解酶基于PCR的方法中的核酸。其中，洗涤剂和清洁产品，例如酶和相应的方法和特别感兴趣的用途。

9. 发明申请

WO2010099955A1 PROTEASE FOR WOUND CONDITIONING AND SKIN CARE 审中-公开
标题翻译：蛋白酶对伤口调理和皮肤护理
公开(公告)号：WO2010099955A1
公开(公告)日：2010-09-10
申请号：PCT/EP2010/001328
申请日：2010-03-03
申请人： B.R.I.A.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , NIEHAUS, Frank , ECK, Jürgen , SCHULZE, Renate
发明人： NIEHAUS, Frank , ECK, Jürgen , SCHULZE, Renate
IPC分类号： C12N9/64 , C07K16/40 , A61K38/43 , C12N15/62 , C12N5/10
CPC分类号： C12N9/6408
摘要： We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds. Described is a nucleic acid molecule encoding a serine protease having the ability to cleave fibrin and casein which is (a) a nucleic acid molecule encoding the serine protease comprising or consisting of the amino acid sequence of SEQ ID NO: 4 as well as to nucleic acid molecules encoding precursors or fragments of said serine protease; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; (c) a nucleic acid molecule encoding a serine protease the amino acid sequence of which is at least 80 % identical to the amino acid sequence of (a), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 80 % identical to the nucleotide sequence of (b), preferably at least 85 % identical, more preferably at least 90 % identical, and most preferred 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (b) or (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.
摘要翻译：我们已经通过分子克隆鉴定了来源于丝光绿蝇幼虫的蛋白酶，并且由于其活性可用于伤口清创，因此被称为清创酶。描述了编码丝氨酸蛋白酶的核酸分子，所述丝氨酸蛋白酶具有切割纤维蛋白和酪蛋白的能力，所述纤维蛋白和酪蛋白是（a）编码包含SEQ ID NO：4的氨基酸序列或由SEQ ID NO：4的氨基酸序列组成的丝氨酸蛋白酶的核酸分子，编码所述丝氨酸蛋白酶的前体或片段的酸分子; （b）包含SEQ ID NO：3的核苷酸序列或由其组成的核酸分子; （c）编码丝氨酸蛋白酶的核酸分子，其氨基酸序列与（a）的氨基酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，且大多数优选的95％相同; （d）包含与（b）的核苷酸序列至少80％相同，优选至少85％相同，更优选至少90％相同，并且最优选95％相同的核苷酸序列或由其组成的核酸分子，相同; （e）相对于（b）或（d）的核酸分子简并的核酸分子; 或（f）对应于（a）至（d）中任一项的核酸分子的核酸分子，其中T被U取代。

10. 发明申请

WO2004018673A2 ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS 审中-公开
标题翻译：来自未受污染的生物体的DNA的分离和克隆
公开(公告)号：WO2004018673A2
公开(公告)日：2004-03-04
申请号：PCT/EP2003/009223
申请日：2003-08-20
申请人： B.R.A.I.N. BIOTECHNOLOGY RESEARCH AND INFORMATION NETWORK AG , QUAISER, Achim , OCHSENREITER, Torsten , TREUSCH, Alexander, H. , KLETZIN, Arnulf , SCHLEPER, Christa , LORENZ, Patrick , ECK, Jürgen
发明人： QUAISER, Achim , OCHSENREITER, Torsten , TREUSCH, Alexander, H. , KLETZIN, Arnulf , SCHLEPER, Christa , LORENZ, Patrick , ECK, Jürgen
IPC分类号： C12N15/10
CPC分类号： C12N15/1003
摘要： The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.
摘要翻译：本发明涉及用于分离和/或纯化核酸分子的装置，所述核酸分子适于结合和/或失活用于DNA操作的试剂或酶的活性的抑制剂并分离多个的核酸分子的大小。此外，本发明涉及用于分离核酸分子的方法，其包括将样品应用于本发明的装置，其中所述核酸分子优选代表给定栖息地的宏基因组的一部分。此外，本发明涉及产生至少一种基因文库的方法，所述基因文库包含通过本发明方法分离的核酸分子和通过本发明方法分离的核酸分子。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式