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1. 发明申请

WO2022170354A1 INTEGRATED AIR FILTERING AND CONDITIONING OF DROPLET CHAMBER IN A COMPACT CELL SORTER 审中-公开
公开(公告)号：WO2022170354A1
公开(公告)日：2022-08-11
申请号：PCT/US2022/070543
申请日：2022-02-05
申请人： CYTEK BIOSCIENCES, INC.
发明人： KRUEGER, Glen , VRANE, David , WANG, Kuncheng , GU, Qiuta
IPC分类号： B01L1/00 , G01N15/14 , G01N35/00
摘要： A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment. A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.

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