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    • 9. 发明申请
    • PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME
    • 具有改进的生长特性的植物及其制备方法
    • WO2006018432A2
    • 2006-02-23
    • PCT/EP2005054034
    • 2005-08-16
    • CROPDESIGN NVFRANKARD VALERIEREUZEAU CHRISTOPHESANZ MOLINERO ANA ISABEL
    • FRANKARD VALERIEREUZEAU CHRISTOPHESANZ MOLINERO ANA ISABEL
    • C12N15/82A01H5/00
    • C12N15/8262C07K14/415C12N15/8261Y02A40/146
    • The present invention concerns a method for improving the growth characteristics of plants by increasing activity in a plant of an RNA-binding protein or a homologue thereof, wherein said RNA-binding protein or homologue thereof is either: (i) a polypeptide having RNA-binding activity and comprising either 2 or 3 RNA 10 recognition motifs (RRMs) and a motif having at least 75% sequence identity to motif I: PIYEAAVVALPVVVKERLVRILRLGIATRYD and/or a motif having at least 50% sequence identity to motif II: RFDPFTGEPYKFDP; or (ii) an RBP1 polypeptide or homologue thereof having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL; and (ii) 15 RPRGFGF, allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% sequence identity to the amino acid represented by SEQ ID NO: 15. The invention also concerns to transgenic plants having introduced therein an RNA-binding protein-encoding nucleic acid or variant thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.
    • 本发明涉及通过增加植物中的RNA结合蛋白或其同源物的活性来改善植物的生长特性的方法,其中所述RNA结合蛋白或其同源物是:(i)具有RNA结合蛋白的多肽, 包含2或3个RNA 10识别基序(RRM)和与基序I具有至少75%序列同一性的基序:PIYEAAVVALPVVKERLVRILRLGIATRYD和/或与基序II具有至少50%序列同一性的基序:RFDPFTGEPYKFDP; 或(ii)具有(a)RNA结合活性的RBP1多肽或其同系物; (b)两个RRM域,(c)以下两个图案:(i)KIFVGGL; 和(ii)15种RPRGFGF,允许多达3个氨基酸取代和图案中任何保守的变化; 和(d)与SEQ ID NO:15所示的氨基酸具有至少20%的序列同一性。本发明还涉及其中引入了编码有RNA结合蛋白的核酸或其变体的转基因植物,其植物已经改进 相对于相应的野生型植物的生长特性。 本发明还涉及可用于本发明方法的构建体。