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    • 2. 发明申请
    • PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME
    • 具有改进的生长特性的植物及其制备方法
    • WO2006018432A2
    • 2006-02-23
    • PCT/EP2005054034
    • 2005-08-16
    • CROPDESIGN NVFRANKARD VALERIEREUZEAU CHRISTOPHESANZ MOLINERO ANA ISABEL
    • FRANKARD VALERIEREUZEAU CHRISTOPHESANZ MOLINERO ANA ISABEL
    • C12N15/82A01H5/00
    • C12N15/8262C07K14/415C12N15/8261Y02A40/146
    • The present invention concerns a method for improving the growth characteristics of plants by increasing activity in a plant of an RNA-binding protein or a homologue thereof, wherein said RNA-binding protein or homologue thereof is either: (i) a polypeptide having RNA-binding activity and comprising either 2 or 3 RNA 10 recognition motifs (RRMs) and a motif having at least 75% sequence identity to motif I: PIYEAAVVALPVVVKERLVRILRLGIATRYD and/or a motif having at least 50% sequence identity to motif II: RFDPFTGEPYKFDP; or (ii) an RBP1 polypeptide or homologue thereof having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL; and (ii) 15 RPRGFGF, allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% sequence identity to the amino acid represented by SEQ ID NO: 15. The invention also concerns to transgenic plants having introduced therein an RNA-binding protein-encoding nucleic acid or variant thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The present invention also concerns constructs useful in the methods of the invention.
    • 本发明涉及通过增加植物中的RNA结合蛋白或其同源物的活性来改善植物的生长特性的方法,其中所述RNA结合蛋白或其同源物是:(i)具有RNA结合蛋白的多肽, 包含2或3个RNA 10识别基序(RRM)和与基序I具有至少75%序列同一性的基序:PIYEAAVVALPVVKERLVRILRLGIATRYD和/或与基序II具有至少50%序列同一性的基序:RFDPFTGEPYKFDP; 或(ii)具有(a)RNA结合活性的RBP1多肽或其同系物; (b)两个RRM域,(c)以下两个图案:(i)KIFVGGL; 和(ii)15种RPRGFGF,允许多达3个氨基酸取代和图案中任何保守的变化; 和(d)与SEQ ID NO:15所示的氨基酸具有至少20%的序列同一性。本发明还涉及其中引入了编码有RNA结合蛋白的核酸或其变体的转基因植物,其植物已经改进 相对于相应的野生型植物的生长特性。 本发明还涉及可用于本发明方法的构建体。
    • 10. 发明申请
    • PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME
    • 具有增强的相关旅行者的植物及其制造方法
    • WO2009080802A2
    • 2009-07-02
    • PCT/EP2008068129
    • 2008-12-22
    • BASF PLANT SCIENCE GMBHSANZ MOLINERO ANA ISABELREUZEAU CHRISTOPHEFRANKARD VALERIEHATZFELD YVES
    • SANZ MOLINERO ANA ISABELREUZEAU CHRISTOPHEFRANKARD VALERIEHATZFELD YVES
    • C12N15/8261Y02A40/146
    • The present invention relates generally to the field of molecular biology and concerns a method for enhancing various yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a TCP1 or a TCP2 transcription factor. The present invention also concerns plants having modulated expression of a nucleic acid encoding a TCP1 or TCP2 polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. In another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an Epsin-like polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an Epsin-like polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising a nucleic acid encoding an Epsin-like polypeptide, useful in performing the methods of the invention. In yet another embodiment, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in the seeds of a plant, of a nucleic acid sequence encoding a tRNA delta(2)- isopentenylpyrophosphate transferase (IPPT) polypeptide. The present invention also concerns plants having increased expression in the seeds, of a nucleic acid sequence encoding an IPPT polypeptide, which plants have increased yield-related traits relative to control plants. The invention additionally relates to nucleic acid constructs, vectors and plants containing said nucleic acid sequences. In further embodiment, the present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants grown under conditions of sub-optimal nutrient availability, comprising modulating expression in a plant of a nucleic acid encoding a SHORT-ROOT (SHR) polypeptide. The present invention also provides a method for increasing Thousand Kernel Weight (TKW) in plants relative to control plants, comprising modulating expression of a nucleic acid encoding an SHR polypeptide in a plant grown under grown under non-nutrient limiting conditions. The present invention also concerns plants having modulated expression of a nucleic acid encoding an SHR polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.
    • 本发明一般涉及分子生物学领域,涉及通过调节植物中编码TCP1或TCP2转录因子的核酸的表达来增强植物中各种产量相关性状的方法。 本发明还涉及具有调节表达编码TCP1或TCP2多肽的核酸的植物,该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 本发明还提供了可用于本发明方法的构建体。 在另一个实施方案中,本发明一般涉及分子生物学领域,并且涉及用于增强植物中各种经济上重要的产量相关性状的方法。 更具体地,本发明涉及一种通过调节植物中编码类似蛋白样样多肽的核酸的表达来增强植物中产量相关性状的方法。 本发明还涉及具有调节编码类似蛋白样样多肽的核酸表达的植物,该植物相对于对照植物具有增强的产量相关性状。 本发明还提供包含可用于实施本发明方法的编码类似蛋白样样多肽的核酸的构建体。 在另一个实施方案中,本发明一般涉及分子生物学领域,并且涉及通过增加植物种子中编码tRNAΔ(2)的核酸序列的表达来增加各种植物产量相关性状的方法, - 异戊烯基焦磷酸转移酶(IPPT)多肽。 本发明还涉及具有增加的种子表达的植物,编码IPPT多肽的核酸序列,该植物相对于对照植物具有增加的产量相关性状。 本发明还涉及含有所述核酸序列的核酸构建体,载体和植物。 在进一步的实施方案中,本发明一般涉及分子生物学领域,并且涉及用于在非最佳营养物质可利用的条件下生长的植物中增强产量相关性状的方法,其包括调节植物中编码短链的核酸的表达 -ROOT(SHR)多肽。 本发明还提供了一种相对于对照植物增加植物中千粒重(TKW)的方法,其包括调节在非营养限制条件下生长的植物中编码SHR多肽的核酸的表达。 本发明还涉及具有编码SHR多肽的核酸调节表达的植物,该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 本发明还提供了可用于本发明方法的构建体。