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1. 发明申请

WO2003060134A1 HERBICIDE MODE OF ACTION DETERMINATION 审中-公开
标题翻译：除草剂作用方式决定
公开(公告)号：WO2003060134A1
公开(公告)日：2003-07-24
申请号：PCT/EP2003/000512
申请日：2003-01-14
申请人： CROPDESIGN N.V. , BROEKAERT, Willem , INZE, Dirk
发明人： BROEKAERT, Willem , INZE, Dirk
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , G01N33/5097
摘要： The present invention relates to methods to determine the molecular target of an agrochemical or candidate agrochemical compound. The methods of the present invention can also be used as screening assay to identify new agrochemicals with pesticidal potency or with growth regulatory potency (such as herbicides or growth stimulators). The invention further relates to agrochemical and agrochemical compositions and methods for preparing these. The invention further provides plants that are tolerant to an agrochemcial compound.
摘要翻译：本发明涉及确定农用化学品或候选农药化合物的分子靶标的方法。本发明的方法也可用作筛选测定以鉴定具有杀虫潜力或具有生长调节效力的新农药（如除草剂或生长刺激剂）。本发明还涉及农用化学品和农药组合物及其制备方法。本发明还提供了耐农药化合物的植物。

2. 发明申请

WO2003025185A1 A METHOD TO MODIFY CELL NUMBER, ARCHITECTURE AND YIELD OF PLANTS BY OVEREXPRESSING THE E2F TRANSCRIPTION FACTOR 审中-公开
标题翻译：通过过度表达E2F转录因子修饰植物细胞数，构建和产量的方法
公开(公告)号：WO2003025185A1
公开(公告)日：2003-03-27
申请号：PCT/EP2002/010236
申请日：2002-09-12
申请人： CROPDESIGN N.V. , BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
发明人： BEECKMAN, Tom , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , BROEKAERT, Willem , DILLEN, Willy , FRANKARD, Valerie
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to a method for modifying plant growth and development processes by affecting cell cycle regulation comprising modulating expression of members of the plant E2F transcription factor family. More specific the invention relates to methods for stimulating cell division in a plant. The invention further relates to transgenic plants produced by the methods of the invention having altered plant growth and development characteristics compared to their isogenic counterparts. Preferably, the plant characteristics modified by the present invention include enlarged cotyledons, enhanced growth rate, stress resistance and seedling survival, more cells, more tillers, more panicles.
摘要翻译：本发明涉及通过影响细胞周期调节来修饰植物生长和发育过程的方法，其包括调节植物E2F转录因子家族成员的表达。更具体地，本发明涉及刺激植物细胞分裂的方法。本发明还涉及通过与其同基因对应物相比具有改变的植物生长和发育特征的本发明方法生产的转基因植物。优选地，通过本发明修饰的植物特征包括扩大的子叶，增强的生长速率，抗胁迫性和幼苗存活，更多的细胞，更多的分蘖，更多的穗。

3. 发明申请

WO2003085115A3 IDENTIFICATION AND VALIDATION OF NOVEL TARGETS FOR AGROCHEMICALS 审中-公开
公开(公告)号：WO2003085115A3
公开(公告)日：2003-10-16
申请号：PCT/EP2003/003703
申请日：2003-04-08
申请人： CROPDESIGN N.V. , INZE, Dirk , BROEKAERT, Willem
发明人： INZE, Dirk , BROEKAERT, Willem
IPC分类号： C12N15/82
摘要： The invention relates to a method for identifying and validating plant targets for agrochemicals, comprising the steps of determining gene or protein expression profiles in function of the progression of an essential biological process in a plant, and the subsequent downregulation of expression of said gene or protein in a plant cell. More particularly, the effects of downregulation of the candidate target gene were directly monitored on plants locally infected with a vector mediating viral induced gene suppression in that infected plant area. The invention also relates to isolated plant genes encoding proteins involved in plant growth and development. The invention also relates to plants tolerant to agrochemicals such as herbicides or pesticides.

4. 发明申请

WO2003085115A2 IDENTIFICATION AND VALIDATION OF NOVEL TARGETS FOR AGROCHEMICALS 审中-公开
标题翻译：鉴定和验证农业新型目标
公开(公告)号：WO2003085115A2
公开(公告)日：2003-10-16
申请号：PCT/EP2003/003703
申请日：2003-04-08
申请人： CROPDESIGN N.V. , INZE, Dirk , BROEKAERT, Willem
发明人： INZE, Dirk , BROEKAERT, Willem
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8274 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , Y02A40/146
摘要： The invention relates to a method for identifying and validating plant targets for agrochemicals, comprising the steps of determining gene or protein expression profiles in function of the progression of an essential biological process in a plant, and the subsequent downregulation of expression of said gene or protein in a plant cell. More particularly, the effects of downregulation of the candidate target gene were directly monitored on plants locally infected with a vector mediating viral induced gene suppression in that infected plant area. The invention also relates to isolated plant genes encoding proteins involved in plant growth and development. The invention also relates to plants tolerant to agrochemicals such as herbicides or pesticides.
摘要翻译：本发明涉及用于鉴定和验证农用化学品的植物靶标的方法，其包括以下步骤：确定基因或蛋白质表达谱，其功能是植物中基本生物学过程的进展，随后下调所述基因或蛋白质的表达在植物细胞中。更具体地说，候选靶基因的下调效应直接由在感染的植物区域中介导病毒诱导的基因抑制的载体局部感染的植物上监测。本发明还涉及编码参与植物生长和发育的蛋白质的分离的植物基因。本发明还涉及耐农业化学品如除草剂或杀虫剂的植物。

5. 发明申请

WO2007054522A1 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改善生长特性的植物及其制备方法
公开(公告)号：WO2007054522A1
公开(公告)日：2007-05-18
申请号：PCT/EP2006/068248
申请日：2006-11-08
申请人： CROPDESIGN N.V. , VIB VZW , UNIVERSITEIT GENT , DE MEUTTER, Jan , MIRONOV, Vladimir , REUZEAU, Christophe , INZE, Dirk , VLIEGHE, Kobe , DE VEYLDER, Lieven
发明人： DE MEUTTER, Jan , MIRONOV, Vladimir , REUZEAU, Christophe , INZE, Dirk , VLIEGHE, Kobe , DE VEYLDER, Lieven
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , C12N15/8271 , Y02A40/146
摘要： The present invention concerns a method for increasing plant yield and/or increased stress resistance by decreasing the level of activity of a DEL1 polypeptide. One such method comprises introducing into a plant a nucleic acid comprising a variant DEL1 nucleic acid. Another method comprises downregulating expression of a DEL1 gene . The invention also relates to transgenic plants having introduced therein a variant DEL1 nucleic acid thereof, which plants have increased yield and/or increased stress resistance relative to control plants. The present invention also concerns a novel DEL1 protein and its encoding sequence, and constructs useful in the methods of the invention.
摘要翻译：本发明涉及通过降低DEL1多肽的活性水平来增加植物产量和/或增加的胁迫抗性的方法。一种这样的方法包括向植物中引入包含变体DEL1核酸的核酸。另一种方法包括下调DEL1基因的表达。本发明还涉及其中引入了其变体DEL1核酸的转基因植物，该植物相对于对照植物具有增加的产量和/或增加的胁迫抗性。本发明还涉及一种新颖的DEL1蛋白及其编码序列，以及可用于本发明方法的构建体。

6. 发明申请

WO2003018818A2 METHOD AND MEANS FOR MODULATING PLANT CELL CYCLE PROTEINS AND THEIR USE IN PLANT CELL GROWTH CONTROL 审中-公开
标题翻译：调节植物细胞周期蛋白的方法和手段及其在植物细胞生长控制中的应用
公开(公告)号：WO2003018818A2
公开(公告)日：2003-03-06
申请号：PCT/EP2002/009504
申请日：2002-08-17
申请人： CROPDESIGN N.V. , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , SEGERS, Gerda
发明人： DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir , SEGERS, Gerda
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , Y02A40/146
摘要： The present invention relates to modulation of endoreduplication in a plant or part thereof which may be achieved by modifying expression or activity of E2F or E2F in combination with DP.Modulation of endoreduplication has an advantageous influence on plant cell size and storage capacity of plant cells.
摘要翻译：本发明涉及通过改变E2F或E2F与DP组合的表达或活性来实现的植物或其部分中的内切复制的调节。内切复制的调节对植物细胞的大小和储存能力具有有利影响。

7. 发明申请

WO2004065596A2 REGULATORY SEQUENCE 审中-公开
标题翻译：监管序列
公开(公告)号：WO2004065596A2
公开(公告)日：2004-08-05
申请号：PCT/EP2004/000645
申请日：2004-01-21
申请人： CROPDESIGN N.V. , HATZFELD, Yves , INZE, Dirk
发明人： HATZFELD, Yves , INZE, Dirk
IPC分类号： C12N15/00
CPC分类号： C12N15/8216
摘要： The present invention relates to the field of plant molecular biology. In particular, it describes the use of a regulatory nucleic acid sequence of the rice gene GOS2 for the regulation of gene expression in plant cells derived from plants other than monocotyledonous plants. The use of the regulatory sequence of the present invention results in constitutive expression with expression levels similar to that of CaMV 35S. The present invention also relates to vectors and host cells comprising these nucleic acid sequences. The invention further relates to transgenic cells and plants comprising these sequences and to methods for obtaining such cells and plants.
摘要翻译：本发明涉及植物分子生物学领域。特别地，其描述了水稻基因GOS2的调节性核酸序列用于调节来自除单子叶植物以外的植物的植物细胞中的基因表达的用途。使用本发明的调节序列导致表达水平与CaMV 35S相似的组成型表达。本发明还涉及包含这些核酸序列的载体和宿主细胞。本发明还涉及包含这些序列的转基因细胞和植物以及获得这种细胞和植物的方法。

8. 发明申请

WO2007051866A3 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
公开(公告)号：WO2007051866A3
公开(公告)日：2007-05-10
申请号：PCT/EP2006/068190
申请日：2006-11-07
申请人： CROPDESIGN N.V. , SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
发明人： SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

9. 发明申请

WO2007051866A2 PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有改进的增长特性的植物及其制备方法
公开(公告)号：WO2007051866A2
公开(公告)日：2007-05-10
申请号：PCT/EP2006/068190
申请日：2006-11-07
申请人： CROPDESIGN N.V. , SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
发明人： SANZ MOLINERO, Ana Isabel , REUZEAU, Christophe , HATZFELD, Yves , BOUDOLF, Veronique , DE VEYLDER, Lieven , INZE, Dirk , MIRONOV, Vladimir
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N9/1205 , C07K14/415 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及相对于相应的野生型植物改善植物生长特性的方法。更具体地说，本发明涉及一种改善植物生长特性的方法，包括调节编码I类同源结构域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸在植物中的表达; 或包括调节编码硝酸盐转运蛋白（NRT）或其同源物的核酸在植物中的表达; 或包括调节编码称为产量增强蛋白16（称为YEP16）的多肽的核酸在植物中的表达; 或包括调节植物中I族糖原合酶激酶（I族类似毛癣菌激酶）或其同系物的表达。本发明还涉及具有调节的表达编码I类同源结构域亮氨酸拉链（HDZip）hox5多肽或其同源物的核酸的植物; 或调节了编码硝酸盐转运蛋白（NRT）或其同源物的核酸的表达; 或调节了表达产量增强蛋白16（以下称为YEP16）的编码多肽的核酸的表达; 或具有调节的第I组糖原合酶激酶（第I组类似物激酶）或其同源物的表达，所述植物相对于相应的野生型植物具有改善的生长特性。本发明还提供了可用于本发明方法的构建体。

10. 发明申请

WO2004035798A3 IDENTIFICATION OF E2F TARGET GENES AND USES THEREOF 审中-公开
公开(公告)号：WO2004035798A3
公开(公告)日：2004-04-29
申请号：PCT/EP2003/011658
申请日：2003-10-20
申请人： CROPDESIGN N.V. , INZE, Dirk , DE VEYLDER, Lieven , VLIEGHE, Kobe
发明人： INZE, Dirk , DE VEYLDER, Lieven , VLIEGHE, Kobe
IPC分类号： C12N15/82
摘要： The present invention concerns a method for altering characteristics of a plant. The invention describes the identification of genes that are upregulated or downregulated in transgenic plants overexpressing E2Fa/DPa and the use of such sequences to alter plant characteristics. A preferred way for altering characteristics of a plant comprises modifying expression of one or more nucleic acid sequences and or modifying level and/or activity of one or more proteins, which nucleic acids and/or proteins are essentially similar to any one or more of SEQ ID NO 1 to 2755. Some of the genes identified in the present invention have an E2Fa target consensus sequence in their 5' upstream region. The identified genes play a role in a variety of biological processes, such as DNA replication, cell wall biosynthesis, nitrogen and/or carbon metabolism, transcription factors etc.

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