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    • 8. 发明申请
    • REGULATORY SEQUENCE
    • 监管序列
    • WO2004065596A2
    • 2004-08-05
    • PCT/EP2004/000645
    • 2004-01-21
    • CROPDESIGN N.V.HATZFELD, YvesINZE, Dirk
    • HATZFELD, YvesINZE, Dirk
    • C12N15/00
    • C12N15/8216
    • The present invention relates to the field of plant molecular biology. In particular, it describes the use of a regulatory nucleic acid sequence of the rice gene GOS2 for the regulation of gene expression in plant cells derived from plants other than monocotyledonous plants. The use of the regulatory sequence of the present invention results in constitutive expression with expression levels similar to that of CaMV 35S. The present invention also relates to vectors and host cells comprising these nucleic acid sequences. The invention further relates to transgenic cells and plants comprising these sequences and to methods for obtaining such cells and plants.
    • 本发明涉及植物分子生物学领域。 特别地,其描述了水稻基因GOS2的调节性核酸序列用于调节来自除单子叶植物以外的植物的植物细胞中的基因表达的用途。 使用本发明的调节序列导致表达水平与CaMV 35S相似的组成型表达。 本发明还涉及包含这些核酸序列的载体和宿主细胞。 本发明还涉及包含这些序列的转基因细胞和植物以及获得这种细胞和植物的方法。
    • 9. 发明申请
    • PLANTS HAVING MODIFIED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME
    • 具有改良增长特性的植物及其制造方法
    • WO2005024029A2
    • 2005-03-17
    • PCT/EP2004/052035
    • 2004-09-03
    • CROPDESIGN N.V.BROEKAERT, WillemFRANKARD, ValerieHATZFELD, YvesMIRONOV, Vladimir
    • BROEKAERT, WillemFRANKARD, ValerieHATZFELD, YvesMIRONOV, Vladimir
    • C12N15/82
    • C12N9/1205C12N15/8262C12Q1/485G01N2333/39G01N2500/10
    • The present invention concerns a method for modifying the growth characteristics of plants by modulating expression in a plant of a B-type CDK nucleic acid and/or modulating activity and/or levels in a plant of a B-type CDK protein. The invention also relates to transgenic plants having modified growth characteristics, which plants have modulated expression of a B-type CDK nucleic acid and/or modulated activity and/or levels in a plant of a B-type CDK protein relative to expression, activity and/or levels in corresponding wild-type plants. The present invention also provides a novel screening method for the identification of mutant CDKs having enhanced CDK activity relative to corresponding non-mutated CDKs. The present invention also provides a novel screening method for the identification of non-active CDKs that are able to bind to CKIs (cyclin dependent kinase inhibitors). The invention also provides mutant CDKs obtainable by the screening methods according to the invention.
    • 本发明涉及通过调节植物中B型CDK核酸的表达和/或调节植物中B的活性和/或水平来修饰植物生长特性的方法 型CDK蛋白。 本发明还涉及具有修饰的生长特征的转基因植物,所述植物调节B型CDK核酸的表达和/或调节B型CDK蛋白的植物中的活性和/或水平相对于表达,活性和 /或相应野生型植物中的水平。 本发明还提供了用于鉴定相对于相应的未突变的CDK具有增强的CDK活性的突变型CDK的新型筛选方法。 本发明还提供了用于鉴定能够结合CKI(细胞周期蛋白依赖性激酶抑制剂)的非活性CDK的新型筛选方法。 本发明还提供了通过根据本发明的筛选方法可获得的突变型CDK。