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1. 发明申请

WO2016188430A8 DEVICES AND METHODS FOR SAMPLE COLLECTION 审中-公开
公开(公告)号：WO2016188430A8
公开(公告)日：2016-12-01
申请号：PCT/CN2016/083295
申请日：2016-05-25
申请人： COYOTE BIOSCIENCE CO., LTD. , COYOTE BIOSCIENCE YIXING CO., LTD.
发明人： LI, Xiang , MU, Xiaobing , HAN, Yuguang , FENG, Huiying , ZHOU, Yang
IPC分类号： C12M1/26 , C12Q1/68
摘要： The present disclosure provides a system for collecting and/or processing a bodily fluid sample of a subject, a method for collecting and/or processing a bodily fluid sample of a subject, and a kit for collecting and/or processing a bodily fluid sample of a subject.

2. 发明申请

WO2016188430A1 DEVICES AND METHODS FOR SAMPLE COLLECTION CROSS-REFERENCE 审中-公开
标题翻译：用于样品收集的装置和方法交叉参考
公开(公告)号：WO2016188430A1
公开(公告)日：2016-12-01
申请号：PCT/CN2016/083295
申请日：2016-05-25
申请人： COYOTE BIOSCIENCE CO., LTD. , COYOTE BIOSCIENCE YIXING CO., LTD.
发明人： LI, Xiang , MU, Xiaobing , HAN, Yuguang , FENG, Huiying , ZHOU, Yang
IPC分类号： C12M1/26 , C12Q1/68
CPC分类号： C12M1/26 , A61B5/150022 , A61B5/150305 , A61B5/150343 , A61B5/150351 , A61B5/150755 , A61B5/150786 , A61B5/15142 , A61B10/00 , A61B10/0045 , B01L3/50 , B01L3/502 , B01L2300/042 , B01L2300/046 , B01L2300/047 , B01L2300/0832 , B01L2400/0406 , B01L2400/0478 , C12Q1/6844 , G01N33/50
摘要： The present disclosure provides a system for collecting and/or processing a bodily fluid sample of a subject, a method for collecting and/or processing a bodily fluid sample of a subject, and a kit for collecting and/or processing a bodily fluid sample of a subject.
摘要翻译：本公开提供了一种用于收集和/或处理受试者的体液样品的系统，用于收集和/或处理受试者的体液样品的方法，以及用于收集和/或处理体液样品的试剂盒课程。

3. 发明申请

WO2016187780A1 DEVICE AND METHOD FOR SAMPLE COLLECTION 审中-公开
标题翻译：用于样品收集的装置和方法
公开(公告)号：WO2016187780A1
公开(公告)日：2016-12-01
申请号：PCT/CN2015/079706
申请日：2015-05-25
申请人： COYOTE BIOSCIENCE YIXING CO., LTD.
发明人： LI, Xiang , MU, Xiaobing , HAN, Yuguang , FENG, Huiying
IPC分类号： A61B5/151 , A61B5/15 , A61M5/00
CPC分类号： A61B5/150022 , A61B5/150099 , A61B5/150343 , A61B5/150412 , A61B5/150572 , A61B5/150755 , A61B5/150916 , A61B5/15113 , A61B5/15142
摘要： A device (100) and a method for collecting a bodily fluid sample of a subject for nucleic acid amplification, and a kit for nucleic acid amplification. The device (100) comprises a device body (102) that is coupled to at least one collection channel (103) including a collection member (105) having an opening at an end thereof to permit flow of the bodily fluid sample from a source thereof to the collection channel (103). The collection channel (103) may be substantially free of an anticoagulant. The device (100) may further comprise a mechanical member (101) coupled to the device body (102). The mechanical member (101) may actuate the collection member (105) to (i) extent away from the collection channel (103) for collecting the bodily fluid sample from the source thereof, and (ii) retract into the collection channel (103) upon collecting the sample.
摘要翻译：用于收集用于核酸扩增的受试者的体液样品的装置（100）和用于核酸扩增的试剂盒的方法。装置（100）包括装置主体（102），该装置本体（102）联接到至少一个收集通道（103），该收集通道（103）包括在其一端具有开口的收集构件（105），以允许体液样品从其源到收集通道（103）。收集通道（103）可以基本上不含抗凝血剂。装置（100）还可以包括耦合到装置本体（102）的机械构件（101）。机械构件（101）可将收集构件（105）致动（i）远离收集通道（103），用于从其源收集体液样品，和（ii）缩回收集通道（103）收集样品。

4. 发明申请

WO2019144966A1 SYSTEMS AND METHODS FOR ANALYZING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2019144966A1
公开(公告)日：2019-08-01
申请号：PCT/CN2019/073621
申请日：2019-01-29
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： CHING, Jesus , LEE, Phillip You Fai , LEE, Alice Wang Yum , LI, Chen , LI, Haojie , LI, Xiang
IPC分类号： C12Q1/68 , C12M1/00
摘要： The present invention provides methods and systems for amplifying and analyzing nucleic acid samples. The method may comprise activating a system comprising a fluid flow network, generating partitions, subjecting the partitions to conditions sufficient to perform a nucleic acid amplification reaction, and detecting signals indicative of a presence or absence of amplification products. Systems to perform the method may comprise a fluid flow network, a plurality of thermal zones, and a detector.

5. 发明申请

WO2017088169A1 METHODS AND SYSTEMS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
公开(公告)号：WO2017088169A1
公开(公告)日：2017-06-01
申请号：PCT/CN2015/095763
申请日：2015-11-27
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： LI, Xiang , WEI, Yumei , LIU, Jiqin
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6846 , Y02A50/451 , Y02A50/53 , Y02A50/54 , C12Q2527/101 , C12Q2527/107
摘要： The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

6. 发明申请

WO2015176674A1 SYSTEMS AND METHODS FOR THERMAL CYCLING CROSS-REFERENCE 审中-公开
标题翻译：用于热循环交叉参考的系统和方法
公开(公告)号：WO2015176674A1
公开(公告)日：2015-11-26
申请号：PCT/CN2015/079499
申请日：2015-05-21
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： LI, Xiang , LI, Chen , HAN, Yuguang , FENG, Huiying
IPC分类号： C12M1/38
CPC分类号： B01L7/52 , B01L2200/18 , B01L2300/0654
摘要： Systems and methods for low voltage power thermal cycling are provided. A nucleic acid amplification device (500) comprising a thermal cycler (510) and a detector (520) is capable of operating using a low operating voltage, such as 12 V. The nucleic acid amplification device (500) may include a portable energy storage device (530) that can provide operating power. The nucleic acid amplification device (500) may be powered by a vehicle (1120) and deployed to remote locations.
摘要翻译：提供了低压功率热循环的系统和方法。包括热循环仪（510）和检测器（520）的核酸扩增装置（500）能够使用诸如12V的低工作电压进行操作。核酸扩增装置（500）可以包括便携式能量储存器设备（530），其可以提供操作功率。核酸扩增装置（500）可以由车辆（1120）供电并且部署到远程位置。

7. 发明申请

WO2017219350A1 SYSTEMS AND METHODS FOR THERMAL CYCLING 审中-公开
标题翻译：用于热循环的系统和方法
公开(公告)号：WO2017219350A1
公开(公告)日：2017-12-28
申请号：PCT/CN2016/087046
申请日：2016-06-24
申请人： COYOTE BIOSCIENCE CO., LTD
发明人： LI, Xiang , YU, Fei
IPC分类号： C12M1/38 , C12M1/36 , C12Q1/68
CPC分类号： C12M1/36 , B01L3/508 , B01L3/50825 , B01L7/52 , B01L2300/043 , B01L2300/047 , B01L2300/185 , C12M1/38 , C12Q1/6844 , C12Q2531/113 , C12Q2561/113
摘要： Nucleic acid amplification systems and methods for performing nucleic acid amplifications with such systems are provided.
摘要翻译：提供了用这样的系统进行核酸扩增的核酸扩增系统和方法。

8. 发明申请

WO2017152840A1 METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2017152840A1
公开(公告)日：2017-09-14
申请号：PCT/CN2017/075955
申请日：2017-03-08
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： LI, Xiang , LI, Chen , YANG, Kun
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L3/502784 , B01L7/52 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/14 , B01L2300/1805 , B01L2400/0487 , C12Q1/686 , C12Q1/6886 , C12Q1/703 , G01N21/05 , G01N21/6452 , G01N21/6456 , G01N2021/035
摘要： The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples.

9. 发明申请

WO2016106689A1 DETECTION OF NUCLEIC ACID MOLECULES USING NANOPORES AND TAGS 审中-公开
标题翻译：使用纳米和标签检测核酸分子
公开(公告)号：WO2016106689A1
公开(公告)日：2016-07-07
申请号：PCT/CN2014/095907
申请日：2014-12-31
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： LI, Xiang , YANG, Kun
IPC分类号： G01N27/406 , G01N27/414 , G01N27/417 , C12Q1/68
CPC分类号： C12Q1/6869 , G01N33/48721 , C12Q2525/204 , C12Q2531/113 , C12Q2531/143 , C12Q2535/122 , C12Q2563/107 , C12Q2563/119 , C12Q2565/631 , C12Q2563/116 , C12Q2563/179
摘要： The present disclosure provides methods and systems for assaying the presence of a target nucleic acid molecule in a sample having or suspected of having the target nucleic acid molecule. A method of assaying the presence of the target nucleic acid molecule comprises facilitating the flow of the sample through at least one nanopore in a membrane disposed adjacent or in proximity to an electrode that detects a current or current change upon movement of the target nucleic acid molecule through the nanopore. The target nucleic acid molecule， if present， can have a tag coupled at a terminal end thereof that increases a dwell time of the target nucleic acid molecule in the nanopore. The presence of the target nucleic acid molecule in the sample is assayed based on an increase in dwell time of the target nucleic acid molecule from measurements of the current or current change.
摘要翻译：本公开提供了用于测定具有或怀疑具有靶核酸分子的样品中靶核酸分子的存在的方法和系统。测定靶核酸分子的存在的方法包括促进样品流过设置在电极附近或附近的膜中的至少一个纳米孔，其检测靶核酸分子移动时的当前或当前变化通过纳米孔。靶核酸分子（如果存在）可以具有在其末端偶联的标签，其增加靶核酸分子在纳米孔中的停留时间。基于当前或当前变化的测量，靶核酸分子的停留时间的增加来测定样品中靶核酸分子的存在。

10. 发明申请

WO2017088834A1 METHODS AND SYSTEMS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：用于核酸扩增的方法和系统
公开(公告)号：WO2017088834A1
公开(公告)日：2017-06-01
申请号：PCT/CN2016/107443
申请日：2016-11-28
申请人： COYOTE BIOSCIENCE CO., LTD.
发明人： LI, Xiang , WEI, Yumei , FENG, Huiying , WANG, Yuning
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6846 , Y02A50/451 , Y02A50/53 , Y02A50/54 , C12Q2527/101 , C12Q2527/107
摘要： Provided are methods and systems for amplifying and analyzing nucleic acid samples.
摘要翻译：提供了扩增和分析核酸样品的方法和系统。

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