专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006065076A1 5'-XMP AMINASE MUTANT 审中-公开
公开(公告)号：WO2006065076A1
公开(公告)日：2006-06-22
申请号：PCT/KR2005/004308
申请日：2005-12-14
申请人： CJ CORPORATION , PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , LEE, Han-Seung , PARK, Young Hoon , KIM, Hyoung Suk , HAN, Jong-Kwon , LEE, Jin Nam , OH, Ki Hoon , KIM, Jeong Hwan , OH, Yoon Suk , SIM, Jae Ick , HONG, Kuk-Ki , CHOI, Kyung Oh , KIM, Hyun Soo , BAEK, Min-Ji , KANG, Tae Sun
发明人： PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , LEE, Han-Seung , PARK, Young Hoon , KIM, Hyoung Suk , HAN, Jong-Kwon , LEE, Jin Nam , OH, Ki Hoon , KIM, Jeong Hwan , OH, Yoon Suk , SIM, Jae Ick , HONG, Kuk-Ki , CHOI, Kyung Oh , KIM, Hyun Soo , BAEK, Min-Ji , KANG, Tae Sun
IPC分类号： C12N9/00 , C12N15/52
CPC分类号： C12N9/93
摘要： The present invention relates to the development of 5'- xanthylic acid (XMP) aminase mutants having enhanced activity using artificial mutagenesis. The 5'-XMP aminase mutants were obtained by performing error-prone PCR to introduce mutations into the native 5'-XMP aminase gene, screening the resulting mutant library for highly active 5'-XMP aminase, performing molecular evolution using the obtained highly active 5'-XMP aminase mutant as a parent gene to obtain 5 ' -XMP aminase having much higher activity, and determining nucleotide sequences of the resulting mutants. The 5'-XMP aminase mutants effectively convert 5 ' -XMP to 5 ' -guanylic acid because they have higher activity than the native form.
摘要翻译：本发明涉及使用人工诱变增强活性的5'-叶酸（XMP）氨基酶突变体的开发。通过进行易错PCR引入天然5'-XMP氨基酶基因的突变获得5'-XMP氨基酸突变体，筛选得到的高活性5'-XMP氨基酶的突变文库，使用获得的高活性进行分子进化 5'-XMP氨基酶突变体作为亲本基因以获得具有高得多活性的5'-XMP氨基酶，并测定所得突变体的核苷酸序列。 5'-XMP氨基酶突变体有效地将5'-XMP转化为5'-鸟苷酸，因为它们具有比天然形式更高的活性。

2. 发明申请

WO2007069861A1 AMMONIA-SPECIFIC 5'-XMP AMINASE MUTANT 审中-公开
标题翻译：氨基酸特异性5'-XMP氨基酸突变体
公开(公告)号：WO2007069861A1
公开(公告)日：2007-06-21
申请号：PCT/KR2006/005475
申请日：2006-12-14
申请人： CJ CORPORATION , PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , LEE, Han-Seung , PARK, Young Hoon , KIM, Hyoung Suk , HAN, Jong-Kwon , LEE, Jin Nam , OH, Ki-Hoon , KIM, Jeong Hwan , OH, Yoon-Suk , SIM, Jae Ick , HONG, Kuk-Ki , CHOI, Kyung Oh , KIM, Hyun Soo , BAEK, Min-Ji , KANG, Tae Sun
发明人： PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , LEE, Han-Seung , PARK, Young Hoon , KIM, Hyoung Suk , HAN, Jong-Kwon , LEE, Jin Nam , OH, Ki-Hoon , KIM, Jeong Hwan , OH, Yoon-Suk , SIM, Jae Ick , HONG, Kuk-Ki , CHOI, Kyung Oh , KIM, Hyun Soo , BAEK, Min-Ji , KANG, Tae Sun
IPC分类号： C12N1/00
CPC分类号： C12N9/78 , C12P19/32
摘要： Disclosed herein are ammonia-specific 5'-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5'-XMP aminase. The resulting 5'-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5'- XMP into 5'-GMP. Thus, the ammonia-specific 5'-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5'-XMP into 5'-GMP.
摘要翻译：本文公开了氨特异性5'-XMP氨基酶突变体及其制备方法。在5'-XMP氨基酶中引入谷氨酰胺依赖性催化活性位点的突变。所得到的5'-XMP氨基酶突变体缺乏谷氨酰胺依赖性活性，并特异性地与外部氨反应将5'- XMP转化为5'-GMP。因此，与野生型相比，氨特异性5'-XMP氨基酶突变体在细胞内更稳定，可用于将5'-XMP工业转化为5'-GMP。

3. 发明申请

WO2008133433A2 INTACT SURFACE DISPLAY OF SUBSTANCES OF INTEREST 审中-公开
标题翻译：感兴趣的物质的完整表面显示
公开(公告)号：WO2008133433A2
公开(公告)日：2008-11-06
申请号：PCT/KR2008/002298
申请日：2008-04-23
申请人： GENOFOCUS CO., LTD. , KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , CHOI, Eun-Hwa , YIM, Sung-Kun
发明人： PAN, Jae-Gu , JUNG, Heung-Chae , KIM, Eui-Joong , CHOI, Eun-Hwa , YIM, Sung-Kun
IPC分类号： C07K17/00
CPC分类号： C07K17/02
摘要： The present invention relates to the method for the intact surface display. More particularly, the present invention relates to a method for displaying the substance of interest on the surface intactly without any genetic modification in cells, which comprises the steps of: (a) preparing a surface donor selected from the group consisting of wild type spores, bacteria and yeasts, wherein the surface donor is not genetically modified; and (b) attaching a substance of interest on the surface of the surface donor by contacting the substance of interest to the surface donor, wherein the substance of interest is proteins, sugars or nucleic acid molecules. According to the present invention, the substance of interest, especially the protein of interest is displayed on the surface of the surface donor very effectively and the protein activity displayed on the surface retains with little or no loss. In addition, the process of the present invention for surface display is relatively convenient and simple.
摘要翻译：
本发明涉及用于完整表面显示的方法。更具体地说，本发明涉及一种完整地在细胞表面展示感兴趣物质而在细胞中没有任何遗传修饰的方法，该方法包括以下步骤：（a）制备选自野生型孢子，细菌和酵母，其中表面供体不是基因修饰的; 和（b）通过使感兴趣的物质与表面供体接触，将感兴趣的物质附着在表面供体的表面上，其中感兴趣的物质是蛋白质，糖或核酸分子。根据本发明，感兴趣的物质，特别是感兴趣的蛋白质非常有效地显示在表面供体的表面上，并且表面上显示的蛋白质活性保持很少或没有损失。另外，本发明的表面显示方法相对简单方便。

4. 发明申请

WO2008020659A1 THERMOPHILIC L-RIBOSE ISOMERASE AND USE THEREOF 审中-公开
标题翻译：热力L-赖氨酸异构体及其用途
公开(公告)号：WO2008020659A1
公开(公告)日：2008-02-21
申请号：PCT/KR2006/003230
申请日：2006-08-17
申请人： PYUN, Yu-Ryang , CHO, Eun-Ah , SEO, Myung-Ji , CHA, Yun-Hwan , KIM, Jeong-Nyeo , LEE, Sang-Jae , HONG, Yong-Ho , PAN, Jae-Gu , JUNG, Heung-Chae
发明人： PYUN, Yu-Ryang , CHO, Eun-Ah , SEO, Myung-Ji , CHA, Yun-Hwan , KIM, Jeong-Nyeo , LEE, Sang-Jae , HONG, Yong-Ho , PAN, Jae-Gu , JUNG, Heung-Chae
IPC分类号： C12N9/90 , C12N15/61
CPC分类号： C12N9/90 , C12P19/02 , C12P19/24
摘要： The present invention relates to a thermophilic L-ribose isomerase which is specifically reactive to L-ribose as substrates, a nucleic acid molecule encoding the same and a method for preparing an aldose or ketose using the same. In recent, L-ribose has been mostly prepared by chemical synthesis methods; however these methods have disadvantages: complexity of purification steps, and production of harmful chemical contaminants and by-products. However, because L-ribose isomerase of this invention has significant thermophilicity as well as substrate specificity to L-ribose, it has plausible advantages over chemical synthesis methods. For example, L-ribose isomerase enables to utilize substrates in higher concentrations since enzymatic reactions using L-ribose isomerase can be carried out with minimizing generation of byproducts in higher temperature conditions. Furthermore, where reactions are performed using the L-ribose isomerizing enzyme of this invention, their viscosity is low, their susceptibility to contamination by microorganisms is very low and their rate is very likely to be high. In addition to this, the L-ribose isomerizing enzyme of this invention contributes to minimization of byproducts generation.
摘要翻译：本发明涉及对L-核糖作为底物特异性反应的嗜热L-核糖异构酶，编码该核酸的核酸分子及使用其的制备醛糖或酮糖的方法。最近，L-核糖主要是通过化学合成方法制备的; 然而，这些方法有缺点：纯化步骤的复杂性以及有害的化学污染物和副产物的生产。然而，由于本发明的L-核糖异构酶具有显着的嗜热性以及对L-核糖的底物特异性，因此它具有优于化学合成方法的合理优点。例如，L-核糖异构酶能够以更高的浓度利用底物，因为使用L-核糖异构酶的酶反应可以在较高温度条件下最小化副产物的产生来进行。此外，当使用本发明的L-核糖异构化酶进行反应时，它们的粘度低，它们对微生物污染的敏感性非常低，并且它们的速率很可能很高。此外，本发明的L-核糖异构化酶有助于产生副产物的最小化。

5. 发明申请

WO2003102203A1 STABILIZED BIOCATALYSTS AND METHODS OF BIOCONVERSION USING THE SAME 审中-公开
标题翻译：稳定的生物化学和使用它的生物转化的方法
公开(公告)号：WO2003102203A1
公开(公告)日：2003-12-11
申请号：PCT/KR2002/000617
申请日：2002-04-09
申请人： GENOFOCUS CO., LTD. , KWON, Seok-Joon , CHOI, Soo-Keun , JUNG, Heung-Chae , PAN, Jae-Gu , CHANG, Joon-Sung , PARK, Tae-Jung
发明人： KWON, Seok-Joon , CHOI, Soo-Keun , JUNG, Heung-Chae , PAN, Jae-Gu , CHANG, Joon-Sung , PARK, Tae-Jung
IPC分类号： C12P1/00
CPC分类号： C12P19/44 , C12N3/00 , C12N11/16 , C12N15/74 , C12N15/75
摘要： The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusionform and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore, displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.
摘要翻译：本发明涉及在孢子或病毒表面上显示的生物催化剂和使用其的生物转化方法，特别涉及使用生物催化剂的生物转化方法，其包括以下步骤：（a）制备用于孢子表面展示的载体，其包含含有编码显示基序的基因和编码生物催化剂的基因的基因构建体，其中，当表达时，所述基因构建体在融合体中表达显示基序和生物催化剂，并且生物催化剂显示在孢子表面上; （b）用载体转染宿主细胞用于孢子表面显示; （c）在宿主细胞的孢子表面上显示生物催化剂; （d）回收孢子，在其表面上显示生物催化剂; 和（e）使用在其表面上显示生物催化剂的孢子和生物催化剂进行生物转化反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式