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1. 发明申请

WO2008020659A1 THERMOPHILIC L-RIBOSE ISOMERASE AND USE THEREOF 审中-公开
标题翻译：热力L-赖氨酸异构体及其用途
公开(公告)号：WO2008020659A1
公开(公告)日：2008-02-21
申请号：PCT/KR2006/003230
申请日：2006-08-17
申请人： PYUN, Yu-Ryang , CHO, Eun-Ah , SEO, Myung-Ji , CHA, Yun-Hwan , KIM, Jeong-Nyeo , LEE, Sang-Jae , HONG, Yong-Ho , PAN, Jae-Gu , JUNG, Heung-Chae
发明人： PYUN, Yu-Ryang , CHO, Eun-Ah , SEO, Myung-Ji , CHA, Yun-Hwan , KIM, Jeong-Nyeo , LEE, Sang-Jae , HONG, Yong-Ho , PAN, Jae-Gu , JUNG, Heung-Chae
IPC分类号： C12N9/90 , C12N15/61
CPC分类号： C12N9/90 , C12P19/02 , C12P19/24
摘要： The present invention relates to a thermophilic L-ribose isomerase which is specifically reactive to L-ribose as substrates, a nucleic acid molecule encoding the same and a method for preparing an aldose or ketose using the same. In recent, L-ribose has been mostly prepared by chemical synthesis methods; however these methods have disadvantages: complexity of purification steps, and production of harmful chemical contaminants and by-products. However, because L-ribose isomerase of this invention has significant thermophilicity as well as substrate specificity to L-ribose, it has plausible advantages over chemical synthesis methods. For example, L-ribose isomerase enables to utilize substrates in higher concentrations since enzymatic reactions using L-ribose isomerase can be carried out with minimizing generation of byproducts in higher temperature conditions. Furthermore, where reactions are performed using the L-ribose isomerizing enzyme of this invention, their viscosity is low, their susceptibility to contamination by microorganisms is very low and their rate is very likely to be high. In addition to this, the L-ribose isomerizing enzyme of this invention contributes to minimization of byproducts generation.
摘要翻译：本发明涉及对L-核糖作为底物特异性反应的嗜热L-核糖异构酶，编码该核酸的核酸分子及使用其的制备醛糖或酮糖的方法。最近，L-核糖主要是通过化学合成方法制备的; 然而，这些方法有缺点：纯化步骤的复杂性以及有害的化学污染物和副产物的生产。然而，由于本发明的L-核糖异构酶具有显着的嗜热性以及对L-核糖的底物特异性，因此它具有优于化学合成方法的合理优点。例如，L-核糖异构酶能够以更高的浓度利用底物，因为使用L-核糖异构酶的酶反应可以在较高温度条件下最小化副产物的产生来进行。此外，当使用本发明的L-核糖异构化酶进行反应时，它们的粘度低，它们对微生物污染的敏感性非常低，并且它们的速率很可能很高。此外，本发明的L-核糖异构化酶有助于产生副产物的最小化。

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