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    • 6. 发明申请
    • PRODUCTION OF ENZYMES FOR LIGNO-CELLULOSIC BIOMASS
    • 生产细胞生物质的酶
    • WO2013190064A1
    • 2013-12-27
    • PCT/EP2013/062936
    • 2013-06-20
    • CHEMTEX ITALIA S.P.A.
    • PARAVISI, StefanoVOLPATI, LauraGIORCELLI, ChiaraRACCAGNI, ElisaOTTONELLO, PieroCHERCHI, Francesco
    • C12P21/00
    • C12N9/2402C12N9/2482C12N9/2494C12P7/10C12P21/00C12Y302/01004C12Y302/01021C12Y302/01037C12Y302/01058C12Y302/01074C12Y302/01091C12Y302/01136Y02E50/16
    • A process produces at least a first enzyme from a host cell, wherein the first enzyme is capable of hydrolyzing a first pre-treated ligno-cellulosic biomass. The process comprises the step of cultivating the host cell to produce at least the first enzyme for a cultivation time, wherein the cultivation of the host cell occurs in a sugar depleted cultivation environment comprising the host cell, water and a solid composition comprising a complex sugar of the solid composition and a lignin of the solid composition. In such a process, the solid composition is obtained from a second pre-treated ligno-cellulosic biomass, comprising a complex sugar of the second pre-treated ligno-cellulosic biomass and a lignin of the second pre-treated ligno-cellulosic biomass; and the ratio of the total amount of the complex sugars of the solid composition to the total amount of the lignin of the solid composition is greater than zero and less than the ratio of the total amount of the complex sugars of the second pre-treated ligno-cellulosic biomass to the total amount of the lignin of the second pre-treated ligno-cellulosic biomass.
    • 一种方法从宿主细胞产生至少第一种酶,其中第一种酶能够水解第一种经预处理的木质纤维素生物质。 该方法包括培养宿主细胞以产生至少第一种酶用于培养时间的步骤,其中宿主细胞的培养发生在包含宿主细胞,水和包含复合糖的固体组合物的贫糖培养环境中 的固体组合物和固体组合物的木质素。 在这种方法中,固体组合物由第二预处理的木质纤维素生物质获得,其包含第二预处理的木质纤维素生物质的复合糖和第二预处理的木质纤维素生物质的木质素; 并且固体组合物的复合糖的总量与固体组合物的木质素总量的比例大于零并且小于第二预处理木质素的复合糖的总量的比例 - 纤维素生物质相对于第二预处理的木质纤维素生物质的木质素的总量。