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1. 发明申请

WO2013136083A1 METHODS FOR OBTAINING LIQUID FROM A SOLID PHASE 审中-公开
标题翻译：从固体相中获取液体的方法
公开(公告)号：WO2013136083A1
公开(公告)日：2013-09-19
申请号：PCT/GB2013/050653
申请日：2013-03-15
申请人： CAMBRIDGE ENTERPRISE LIMITED
发明人： CHUA, Yii Leng
IPC分类号： C12N15/10
CPC分类号： B01D15/20 , B01D11/02 , B01D11/0219 , B01D15/00 , B01D15/02 , B01D15/24 , B01D15/245 , B01D15/26 , B01D17/02 , B01D17/0202 , C12N15/1006 , C12N15/101 , C12N15/1017
摘要： A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilise capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.
摘要翻译：描述了从多孔固相中获得液体的方法。该方法包括在多孔固相的第一端形成液体密封，液体与液体结合在一起，其中液体密封液体与与固相结合的液体不混溶，并在多孔固相上施加压力差以使不相容的液体朝向多孔固相的第二端移动通过多孔固相，从而将结合到多孔固相的液体移向第二端并从第二端释放该液体。与其中没有形成液体密封的相应方法相比，使用这种方法从固相中回收液体。在优选的实施方案中，用于形成液体密封的液体是矿物油。该方法在利用核酸捕获到固相的核酸提取中具有特别的应用。还描述了用于执行该方法的套件和装置。

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