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1. 发明申请

WO1993019172A1 METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS 审中-公开
标题翻译：用于生产特定绑定对的成员的方法
公开(公告)号：WO1993019172A1
公开(公告)日：1993-09-30
申请号：PCT/GB1993000605
申请日：1993-03-24
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL , JOHNSON, Kevin, Stuart , WINTER, Gregory, Paul , GRIFFITHS, Andrew, David , SMITH, Andrew, John, Hammond , WATERHOUSE, Peter
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/00
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/40 , C07K16/4208 , C07K16/462 , C07K16/468 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62
摘要： Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the l &cir& _o &cir& _x &cir& _P sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
摘要翻译：公开了用于产生特异性结合对（sbp）成员的重组宿主细胞和试剂盒，例如，抗体，使用分泌的可复制遗传显示包（rgdps）的表面上的显示。丝状噬菌体为了产生大量多样性的文库，在包含分别编码sbp成员的第一和第二多肽链的核酸的第一和第二载体之间发生重组，从而产生每个编码sbp成员的第一和第二多肽链组分的重组载体。重组可以在体外或细胞内发生，并且可以是位点特异性的，例如，涉及使用l＆cir＆_o＆cir＆_x＆cir＆_P序列及其突变体。重组可能在先前的筛选或选择rgdps显示sbp成员之后发生，该成员绑定了感兴趣的补充sbp成员。

2. 发明申请

WO1992020791A1 METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS 审中-公开
标题翻译：用于生产特定绑定对的成员的方法
公开(公告)号：WO1992020791A1
公开(公告)日：1992-11-26
申请号：PCT/GB1992000883
申请日：1992-05-15
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL , WINTER, Gregory, Paul , JOHNSON, Kevin, Stuart , GRIFFITHS, Andrew, David , SMITH, Andrew, John, Hammond
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/00
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , G01N33/53 , G01N33/6857
摘要： The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display packages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genetic material of rgdps produced encodes a polypeptide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combinations. In one embodiment of the invention a method employs "chain shuffling" in the production of sbp members of desired specificity for a counterpart sbp member. Selection procedures are also described.
摘要翻译：本发明提供用于产生特异性结合对（sbp）成员的方法和试剂盒。将sbp成员的多肽链组分的群体组合以形成由分泌的可复制的遗传显示包（rgdp）显示的sbps的文库。至少一个多肽链表达为与rgdp的组分的融合物，由此在rgdp的表面显示该多肽链。至少一个多肽链群由能够使用rgdp的组分包装的核酸表达，由此产生的rgdps的遗传物质编码多肽链。该方法能够从非常大量的可能组合生产多聚体sbp成员的文库。在本发明的一个实施方案中，一种方法在生产对于对应物质成员的所需特异性的sbp成员中使用“链洗牌”。还描述了选择程序。

3. 发明申请

WO1992001047A1 METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS 审中-公开
标题翻译：用于生产特定绑定对的成员的方法
公开(公告)号：WO1992001047A1
公开(公告)日：1992-01-23
申请号：PCT/GB1991001134
申请日：1991-07-10
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL , McCAFFERTY, John , POPE, Anthony, Richard , JOHNSON, Kevin, Stuart , HOOGENBOOM, Hendricus, Renerus, Jacobus, Mattheus , GRIFFITHS, Andrew, David , JACKSON, Ronald, Henry , HOLLIGER, Kaspar, Philipp , MARKS, James, David , CLACKSON, Timothy, Piers , CHISWELL, David, John , WINTER, Gregory, Paul , BONNERT, Timothy, Peter
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/00
CPC分类号： C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.
摘要翻译：特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

4. 发明申请

WO1993011236A1 PRODUCTION OF ANTI-SELF ANTIBODIES FROM ANTIBODY SEGMENT REPERTOIRES AND DISPLAYED ON PHAGE 审中-公开
标题翻译：从抗体部分代码产生抗自身抗体并在噬菌体上显示
公开(公告)号：WO1993011236A1
公开(公告)日：1993-06-10
申请号：PCT/GB1992002240
申请日：1992-12-02
申请人： MEDICAL RESEARCH COUNCIL , CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , GRIFFITHS, Andrew, David , HOOGENBOOM, Hendricus, Renerus, Jacobus, Mattheus , MARKS, James, David , McCAFFERTY, John , WINTER, Gregory, Paul , GRIGG, Geoffrey, Walter
发明人： MEDICAL RESEARCH COUNCIL , CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： C12N15/13
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/26 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/3092 , C07K16/34 , C07K16/40 , C07K16/4208 , C07K16/4241 , C07K16/44 , C07K16/462 , C07K16/468 , C07K2317/21 , C07K2317/24 , C07K2317/55 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , Y10S530/867
摘要： Methods are disclosed for the production of anti-self antibodies and antibody fragments, being antibodies or fragments of a particular species of mammal which bind self-antigens of that species. Methods comprise providing a library of replicable genetic display packages (rgdps), such as filamentous phage, each rgdp displaying at its surface a member of a specific binding pair which is an antibody or antibody fragment, and each rgdp containing nucleic acid sequence derived from a species of mammal. The nucleic acid sequence in each rgdp encodes a polypeptide chain which is a component part of the sbp member displayed at the surface of that rgdp. Anti-self antibody fragments are selected by binding with a self antigen from the said species of mammal. The displayed antibody fragments may be scFv, Fd, Fab or any other fragment which has the capability of binding antigen. Nucleic acid libraries used may be derived from a rearranged V-gene sequences of unimmunised mammal. Synthetic or artificial libraries are described and shown to be useful.
摘要翻译：公开了用于产生抗自身抗体和抗体片段的方法，其是结合该物种的自身抗原的哺乳动物特定种类的抗体或片段。方法包括提供可复制的遗传显示包（rgdps）文库，例如丝状噬菌体，每个rgdp在其表面上显示作为抗体或抗体片段的特异性结合对的成员，以及每个含rgdp的核酸序列衍生自哺乳动物的种类每个rgdp中的核酸序列编码多肽链，其是在该rgdp的表面显示的sbp成员的组成部分。通过与来自所述哺乳动物种的自身抗原结合来选择抗自身抗体片段。显示的抗体片段可以是scFv，Fd，Fab或具有结合抗原能力的任何其它片段。所使用的核酸文库可以来源于未免疫的哺乳动物的重新排列的V基因序列。描述和显示合成或人造文库是有用的。

5. 发明申请

WO1994013804A1 MULTIVALENT AND MULTISPECIFIC BINDING PROTEINS, THEIR MANUFACTURE AND USE 审中-公开
标题翻译：多功能和多重结合蛋白质，其制造和使用
公开(公告)号：WO1994013804A1
公开(公告)日：1994-06-23
申请号：PCT/GB1993002492
申请日：1993-12-03
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL , HOLLIGER, Kaspar-Philipp , GRIFFITHS, Andrew, David , HOOGENBOOM, Hendricus, Renerus, Jacobus, Matheus , MALMQVIST, Magnus , MARKS, James, David , McGUINNESS, Brian, Timothy , POPE, Anthony, Richard , PROSPERO, Terence, Derek , WINTER, Gregory, Paul
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , MEDICAL RESEARCH COUNCIL
IPC分类号： C12N15/13
CPC分类号： C07K16/005 , A61K38/00 , C07K16/00 , C07K16/40 , C07K16/44 , C07K16/468 , C07K2317/34 , C07K2317/622 , C07K2317/626 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/113 , C12N2310/111 , C12N2310/1241 , C12N2310/127 , C40B40/02
摘要： Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.
摘要翻译：包含第一结构域的多肽，其包含免疫球蛋白重链可变区的结合区和第二结构域，其包含免疫球蛋白轻链可变区的结合区，所述结构域被连接但不能彼此缔合形成抗原结合位点，缔合以形成抗原结合多聚体，例如二聚体，其可以是多价的或具有多特异性。结构域可以通过短肽接头连接，或者可以直接连接在一起。双特异性二聚体可以具有更长的接头。公开了多肽和多聚体及其多种组成成分的制备方法，以及它们在噬菌体表面上的显示以便于选择感兴趣的粘合剂，以及许多实用性。

6. 发明申请

WO1993006213A1 PRODUCTION OF CHIMERIC ANTIBODIES - A COMBINATORIAL APPROACH 审中-公开
标题翻译：生产抗体 - 组合方法
公开(公告)号：WO1993006213A1
公开(公告)日：1993-04-01
申请号：PCT/GB1992001755
申请日：1992-09-23
申请人： MEDICAL RESEARCH COUNCIL , CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , HOOGENBOOM, Hendricus, Renerus, Jacobus, Matteus , BAIER, Michael , JESPERS, Laurent, Stephane, Anne, Therese , WINTER, Gregory, Paul
发明人： MEDICAL RESEARCH COUNCIL , CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： C12N15/00
CPC分类号： C40B40/02 , B01J2219/00718 , C07K16/00 , C07K16/005 , C07K16/18 , C07K16/22 , C07K16/241 , C07K16/28 , C07K16/2812 , C07K16/2863 , C07K16/2866 , C07K16/3007 , C07K16/40 , C07K16/4208 , C07K16/462 , C07K16/468 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/622 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037
摘要： Methods are disclosed which may be used for the production of antibodies, or antibody fragments, which have the same binding specificity as a parent antibody but which have increased human characteristics. Humanised antibodies may be obtained by chain shuffling, perhaps using phage display technology. In one embodiment, a polypeptide comprising a heavy or light chain variable domain of a non-human antibody specific for an antigen of interest is combined with a repertoire of human complementary (light or heavy) chain variable domains. Hybrid pairings which are specific for the antigen of interest are selected. Human chains from the selected pairings may then be combined with a repertoire of human complementary variable domains (heavy or light) and humanised antibody polypeptide dimers can then be selected for binding specificity for antigen. The methods may be combined with CDR-imprinting. In another embodiment, component part of an antigen-binding site of a non-human antibody known to bind a particular antigen is combined with a repertoire of component parts of an antigen-binding site of human antibody, forming a library of antibody polypeptide dimers with antigen-binding sites. Hybrids selected from this library may be used in a second humanising shuffling step, or may already be of sufficient human character to be of value, perhaps after some modification to increase human character still further.
摘要翻译：公开了可用于生产具有与亲本抗体相同的结合特异性但具有增加的人类特征的抗体或抗体片段的方法。人源化抗体可以通过链式洗牌获得，也许使用噬菌体展示技术。在一个实施方案中，将包含感兴趣抗原特异性的非人抗体的重链或轻链可变结构域的多肽与人互补（轻链或重链）可变域的所有组成部分组合。选择对感兴趣的抗原特异的杂交配对。然后将所选配对的人链与人互补可变结构域（重或轻）的组合组合，然后可以选择人源化抗体多肽二聚体以结合抗原的特异性。这些方法可以与CDR-印记组合。在另一个实施方案中，已知结合特定抗原的非人抗体的抗原结合位点的组成部分与人抗体的抗原结合位点的组成部分的组成部分组合，形成抗体多肽二聚体文库，抗原结合位点。从该图书馆中选出的杂种可以用于第二次人性化洗牌步骤中，或者可能已经具有足够的人性以具有价值，可能在经过一些修改以增加人的性格之后。

7. 发明申请

WO1997013844A1 SPECIFIC BINDING MEMBERS FOR HUMAN TRANSFORMING GROWTH FACTOR BETA; MATERIALS AND METHODS 审中-公开
标题翻译：用于人体变形生长因子BETA的特定结合成员; 材料和方法
公开(公告)号：WO1997013844A1
公开(公告)日：1997-04-17
申请号：PCT/GB1996002450
申请日：1996-10-07
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , THOMSON, Julia, Elizabeth , VAUGHAN, Tristan, John , WILLIAMS, Andrew, James , GREEN, Jonathan, Alexander , JACKSON, Ronald, Henry , BACON, Louise , JOHNSON, Kevin, Stuart , WILTON, Alison, Jane , TEMPEST, Philip, Ronald , POPE, Anthony, Richard
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： C12N05/10
CPC分类号： C07K16/22 , A61K38/00 , C07K2317/21 , C07K2317/622
摘要： Specific binding members comprising human antibody antigen binding domains specific for human transforming growth factor beta (TGF beta ) bind specifically isoforms TGF beta 2 and TGF beta 1 or both, preferentially compared with TGF beta 3. Specific binding members may be isolated and utilised in the treatment of disease, particularly fibrotic disease and also immune/inflammatory diseases. Therapeutic utility is demonstrated using in vitro and in vivo models. Full sequence and binding information is provided, including epitope sequence information for a particularly advantageous specific binding member which binds the active form of TGF beta 2, neutralising its activity, but does not bind the latent form.
摘要翻译：包含对人转化生长因子β（TGFβ）特异性的人抗体结合结构域的特异性结合成员与TGFβ3优先结合，特异性结合特异性同种型TGFβ2和TGFβ1或两者。特异性结合成员可以被分离并用于治疗疾病，特别是纤维化疾病以及免疫/炎症疾病。使用体外和体内模型证明治疗效用。提供了完整的序列和结合信息，包括结合TGFβ2的活性形式的特别有利的特异性结合成员的表位序列信息，中和其活性，但不结合潜伏形式。

8. 发明申请

WO1992001787A1 BINDING DOMAINS 审中-公开
标题翻译：绑定域
公开(公告)号：WO1992001787A1
公开(公告)日：1992-02-06
申请号：PCT/GB1991001253
申请日：1991-07-25
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , JOHNSON, Kevin, Stuart , JACKSON, Ronald, Henry , CHISWELL, David, John
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： C12N15/12
CPC分类号： C07K16/00 , A61K38/00 , C07K14/705
摘要： This invention concerns binding domains e.g. single chain variable domains which are synthetic analogues of other single chain variable domains of members of an immunoglobulin family or superfamily. In the analogue, one or more amino acid residues is altered as compared to the other domain, so that the analogue is more hydrophilic than the natural domain. The altered amino acid is substituted with a residue which occurs in an analogous position in a member of an immunoglobulin family or superfamily. This increased hydrophilicity means that the synthetic analogue will show less non-specific binding than the natural domain. The analogue may retain the binding specificity of the natural domain. Alternatively, the complementarity determining regions may be altered to change the binding specificity. The invention also concerns methods for making these binding domains.
摘要翻译：本发明涉及结合域，例如单链可变结构域，其是免疫球蛋白家族成员或超家族的其它单链可变结构域的合成类似物。在类似物中，与其他结构域相比，一个或多个氨基酸残基被改变，使得类似物比天然结构域更亲水。改变的氨基酸被以在免疫球蛋白家族成员或超家族中的类似位置发生的残基取代。这种增加的亲水性意味着合成类似物将显示比天然结构域更少的非特异性结合。类似物可以保留天然结构域的结合特异性。或者，可以改变互补决定区以改变结合特异性。本发明还涉及制备这些结合结构域的方法。

9. 发明申请

WO1998001757A1 LABELLING AND SELECTION OF MOLECULES 审中-公开
标题翻译：标签和选择分子
公开(公告)号：WO1998001757A1
公开(公告)日：1998-01-15
申请号：PCT/GB1997001835
申请日：1997-07-08
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , OSBOURN, Jane, Katharine , DERBYSHIRE, Elaine, Joy , McCAFFERTY, John, Gerald , VAUGHAN, Tristan, John , JOHNSON, Kevin, Stuart
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： G01N33/535
CPC分类号： G01N33/532 , A61K38/00 , C07K1/36 , C07K14/523 , C07K14/70542 , C07K14/70596 , C07K14/82 , C07K16/00 , C07K16/065 , C07K16/22 , C07K16/2854 , C07K16/2866 , C07K16/2896 , C07K16/3007 , C07K16/32 , C07K16/4266 , C07K2317/34 , C07K2317/622 , C12N9/16 , G01N33/535
摘要： A method of labelling molecules which includes providing in a common medium a label molecule, a marker ligand able to bind a member of a specific binding pair, such as an antigen, a sbp member, an enzyme able to catalyse binding of the label molecule to other molecules, the enzyme being associated with the marker ligand; causing or allowing binding of the marker ligand to the sbp member; and causing or allowing binding of the label molecule to other molecules in the vicinity of the marker ligand bound to the sbp member. The marker ligand may be an antibody or any specific binding molecule, such as a chemokine or cytokine. A complementary member of the specific binding pair may be included, e.g. an antibody, or a diverse population of such sbp members, e.g. antibodies, may be included within which those which bind the counterpart sbp member, e.g. antigen, may be labelled and subsequently isolated for manipulation and/or use. Suitable labels include biotin-tyramine with signal transfer being catalysed by hydrogen peroxidase. Cells, virus particles and other moieties may be labelled, for identification or obtention of proteins which interact or are in close proximity with a particular sbp member, or of cells of interest, or for enhancement of labelling, e.g. for cell sorting.
摘要翻译：标记分子的方法，其包括在普通培养基中提供标记分子，能够结合特异性结合对成员的标记配体，例如抗原，sbp成员，能够催化标记分子结合的酶其他分子，酶与标记配体相关; 引起或允许标记配体与sbp构件的结合; 并引起或允许标记分子与结合于sbp构件的标记配体附近的其它分子结合。标记物配体可以是抗体或任何特异性结合分子，例如趋化因子或细胞因子。可以包括特异性结合对的互补成员，例如。抗体，或这样的sbp成员的不同群体，例如。可以包括其中结合对应物sbp成员的那些抗体。抗原，可以被标记并随后分离用于操作和/或使用。合适的标记包括具有由过氧化氢酶催化的信号传递的生物素 - 酪胺。可以标记细胞，病毒颗粒和其它部分，用于鉴定或获得与特定sbp成员或感兴趣的细胞相互作用或紧密接近的蛋白质，或用于增强标记，例如，用于细胞分选。

10. 发明申请

WO1992011031A1 TARGETTING IgE EFFECTOR CELLS TO TUMOR CELLS 审中-公开
标题翻译：将IgE效应细胞靶向肿瘤细胞
公开(公告)号：WO1992011031A1
公开(公告)日：1992-07-09
申请号：PCT/GB1991002303
申请日：1991-12-23
申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED , JOHNSON, Kevin, Stuart , CHISWELL, David, John
发明人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED
IPC分类号： A61K39/395
CPC分类号： C07K16/468
摘要： The invention relates to binding molecules capable of recruiting IgE effector functions. The invention provides molecules having a first binding domain capable of binding to a tumour antigen and a second binding domain capable of binding to a Fc epsilon receptor on cells of the immune system, whereby the molecule is able to mediate IgE effector mechanisms against a tumour cell expressing the antigen.
摘要翻译：本发明涉及能够募集IgE效应子功能的结合分子。本发明提供了具有能够结合肿瘤抗原的第一结合结构域和能够结合免疫系统细胞上的Fcε受体的第二结合结构域的分子，由此该分子能够介导针对肿瘤细胞的IgE效应机制表达抗原。

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