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1. 发明申请

WO0190346A3 GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT 审中-公开
标题翻译：基因重组和杂交蛋白质的开发
公开(公告)号：WO0190346A3
公开(公告)日：2002-10-10
申请号：PCT/US0116831
申请日：2001-05-23
申请人： CALIFORNIA INST OF TECHN , WANG ZHEN-GANG , VOIGT CHRISTOPHER A , MAYO STEPHEN L , ARNOLD FRANCES H
发明人： WANG ZHEN-GANG , VOIGT CHRISTOPHER A , MAYO STEPHEN L , ARNOLD FRANCES H
IPC分类号： C12N15/10 , G06F19/22 , G06F17/50 , G06F19/00
CPC分类号： G06F19/14 , C12N15/1027 , G06F19/22
摘要： The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.
摘要翻译：本发明涉及聚合物定向进化的改进方法，包括核酸和蛋白质的定向进化。具体地，本发明的方法包括用于识别聚合物中的“交叉位置”的分析方法。这些位置处的交叉链不太可能破坏蛋白质的所需性质，例如稳定性或功能性。本发明还提供了用于定向进化的改进方法，其中聚合物在所识别的“交叉位置”选择性重组。交叉中断配置文件可用于标识首选交叉位置。还可以鉴定和分析生物聚合物的结构域，并将结构域组织成模式。可以例如基于构象能量或原子间距离来计算模式中断简档，并且这些可以用于识别优选或候选交叉位置。还提供了用于实现本发明的分析方法的计算机系统。

2. 发明申请

WO2011153276A3 STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASE CLASS I ENZYMES 审中-公开
标题翻译：稳定的，功能性的二氧化碳氢化酶I级酶
公开(公告)号：WO2011153276A3
公开(公告)日：2012-04-12
申请号：PCT/US2011038812
申请日：2011-06-01
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , HEINZELMAN PETE
发明人： ARNOLD FRANCES H , HEINZELMAN PETE
IPC分类号： C07K19/00 , C12N15/55 , C12N15/62 , C12P19/04
CPC分类号： C12P19/14 , C07K2319/35 , C12N9/2437 , C12P19/02 , C12Y302/01004 , C12Y302/01074 , C12Y302/01091
摘要： The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及CBH I嵌合融合多肽，编码多肽的核酸和用于产生多肽的宿主细胞。

3. 发明申请

WO2008016709A9 METHODS AND SYSTEMS FOR SELECTIVE FLUORINATION OF ORGANIC MOLECULES 审中-公开
标题翻译：用于选择性氟化有机分子的方法和系统
公开(公告)号：WO2008016709A9
公开(公告)日：2008-05-29
申请号：PCT/US2007017409
申请日：2007-08-04
申请人： CALIFORNIA INST OF TECHN , FASAN RUDI , ARNOLD FRANCES H
发明人： FASAN RUDI , ARNOLD FRANCES H
IPC分类号： C12P5/02 , C12P1/00
CPC分类号： C07B39/00 , C07C17/16 , C07C45/63 , C07C49/687 , C07H5/02 , C12P7/00 , C12P7/42 , C12P7/62 , C07C23/20
摘要： A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.
摘要翻译：用于选择性氟化靶位点上的有机分子的方法和系统，其中靶位点被活化然后氟化，连同用于鉴定具有生物活性的分子的方法和系统一起显示。

4. 发明申请

WO0010007A9 DEVICES AND METHODS FOR ANALYSIS OF NON-IONIC SOLUTES 审中-公开
标题翻译：用于分析非离子溶液的装置和方法
公开(公告)号：WO0010007A9
公开(公告)日：2000-07-06
申请号：PCT/US9918615
申请日：1999-08-16
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , MICHAELS ALAN S , ZHENG WEIGONG
发明人： ARNOLD FRANCES H , MICHAELS ALAN S , ZHENG WEIGONG
IPC分类号： G01N27/30 , G01N33/487 , A61B5/00
CPC分类号： G01N27/3271
摘要： This invention relates to devices and methods for detecting and monitoring the concentration, level or amount of a target molecule in solution. More particularly, the invention is directed to sensors that evaluate changes in conductivity produced by the reaction of a target molecule with a sensor composition. The invention is particularly well suited for determining the concentration of carbohydrate compounds in solution, such as diol compounds, preferably biologically significant cis-diols such as various sugars including glucose. The invention also encompasses sensors for determining the total or relative amount of target molecule in a sample, particularly a biological sample such as blood, urine, or other biological fluids. A laminated sensor of the invention comprises a sensor composition applied to a metal electrode, such as an interdigitated gold electrode on a non-conductive support. The sensor composition and electrode are and preferably encapsulated by a bipolar membrane. In a preferred embodiment, the sensor composition is a blend of a boronic acid polymer and a non-ionogenic hydrogel matrix.
摘要翻译：本发明涉及用于检测和监测溶液中靶分子的浓度，水平或量的装置和方法。更具体地说，本发明涉及评估由目标分子与传感器组合物反应产生的电导率变化的传感器。本发明特别适用于测定溶液中碳水化合物的浓度，例如二醇化合物，优选生物学上重要的顺式二醇，如各种糖，包括葡萄糖。本发明还包括用于测定样品中特别是生物样品如血液，尿液或其他生物液体中目标分子的总量或相对量的传感器。本发明的层压传感器包括施加到金属电极上的传感器组合物，例如非导电支撑体上的叉指金电极。传感器组合物和电极优选被双极膜封装。在优选的实施方案中，传感器组合物是硼酸聚合物和非离子水凝胶基质的混合物。

5. 发明申请

WO2008121435A3 METHODS FOR GENERATING NOVEL STABILIZED PROTEINS 审中-公开
标题翻译：生成新型稳定蛋白的方法
公开(公告)号：WO2008121435A3
公开(公告)日：2009-04-23
申请号：PCT/US2008052795
申请日：2008-02-01
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , LI YOUGEN
发明人： ARNOLD FRANCES H , LI YOUGEN
IPC分类号： C07K14/00
CPC分类号： C12N9/0079 , C12N9/0042
摘要： Methods for identifying and producing stabilized chimeric proteins based on cytochrome P540 are disclosed. Included are polypeptides from the CYP102A1, CYP102A2, and CYP102A3 and combinations thereof. Peptide segments, polynucleotides, vectors, host cells, and enzyme extracts are also disclosed.
摘要翻译：公开了基于细胞色素P540鉴定和产生稳定的嵌合蛋白的方法。包括来自CYP102A1，CYP102A2和CYP102A3的多肽及其组合。还公开了肽段，多核苷酸，载体，宿主细胞和酶提取物。

6. 发明申请

WO03008563A9 IMPROVED CYTOCHROME P450 OXYGENASES 审中-公开
标题翻译：改进的CYTOCHROME P450氧化酶
公开(公告)号：WO03008563A9
公开(公告)日：2003-08-14
申请号：PCT/US0223340
申请日：2002-07-22
申请人： CALIFORNIA INST OF TECHN , FARINAS EDGARDO T , GLIEDER ANTON , ARNOLD FRANCES H , SCHWANEBERG ULRICH
发明人： FARINAS EDGARDO T , GLIEDER ANTON , ARNOLD FRANCES H , SCHWANEBERG ULRICH
IPC分类号： C12N9/02 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , C12N
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。与相应的野生型或亲本细胞色素P450酶相比，细胞色素P450变体具有更高的烷烃氧化能力，烯烃氧化能力和/或更高的有机溶剂抗性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改进的包含少于8个碳的烷烃和环氧化物烯烃的羟化能力并且具有对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些。优选的细胞色素P450变体还包括在包含共溶剂例如DMSO和THF的溶液中具有改善的羟基化活性并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代的那些。

7. 发明申请

WO9960096A9 OXYGENASE ENZYMES AND SCREENING METHOD 审中-公开
标题翻译：氧化酶和筛选方法
公开(公告)号：WO9960096A9
公开(公告)日：2000-02-24
申请号：PCT/US9911460
申请日：1999-05-21
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , JOO HYUN , LIN ZHANGLIN
发明人： ARNOLD FRANCES H , JOO HYUN , LIN ZHANGLIN
IPC分类号： C12N9/02 , C12N9/08 , C12N15/10 , C12N15/67 , C12N15/81 , C12Q1/26 , C12Q1/28 , C12N
CPC分类号： C12N9/0071 , C07K2319/02 , C12N9/0065 , C12N15/102 , C12N15/1034 , C12N15/1058 , C12N15/1086 , C12N15/67 , C12N15/815 , C12Q1/26 , C12Q1/28 , G01N2333/795 , G01N2333/80 , G01N2333/90245
摘要： A method for detecting the presence of an oxygenated compound which is produced when a substrate is reacted with an oxygenase for the substrate. The method involves reacting a coupling enzyme with the oxygenated compound to form a polymeric oxygenated compound which is fluorescent or luminescent. Measurement of the fluorescence or luminescence of the polymeric oxygenated compound provides indirect detection of the oxygenated compound produced by reaction of the oxygenase with the substrate. The method is carried out in a whole cell environment wherein the cell is transformed to express both the oxygenase being screened and the coupling enzyme. The method can be used to measure the activity of monooxygenases and dioxygenases on aromatic substrates. The method is amenable to large scale screening of enzyme mutants to isolate those with maximum oxygenase activity.
摘要翻译：用于检测当底物与用于底物的加氧酶反应时产生的含氧化合物的存在的方法。该方法包括使偶联酶与含氧化合物反应以形成荧光或发光的聚合的氧化化合物。聚合物氧化化合物的荧光或发光的测量提供了通过加氧酶与底物的反应产生的氧合化合物的间接检测。该方法在整个细胞环境中进行，其中转化细胞以表达被筛选的加氧酶和偶联酶。该方法可用于测量单加氧酶和双加氧酶在芳香族底物上的活性。该方法适用于大规模筛选酶突变体以分离具有最大加氧酶活性的那些。

8. 发明申请

WO2010091441A2 STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASES 审中-公开
标题翻译：稳定的，功能性的嵌合体纤维水解物
公开(公告)号：WO2010091441A2
公开(公告)日：2010-08-12
申请号：PCT/US2010027248
申请日：2010-03-12
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , HEINZELMAN PETE , MINSHULL JEREMY , GOVINDARAJAN SRIDHAR , VILLALOBOS ALAN
发明人： ARNOLD FRANCES H , HEINZELMAN PETE , MINSHULL JEREMY , GOVINDARAJAN SRIDHAR , VILLALOBOS ALAN
IPC分类号： C12N9/14 , C07K19/00 , C12N15/55 , C12N15/62
CPC分类号： C12P19/02 , C12N9/2437 , C12N15/1089 , C12N15/62 , C12Y302/01091
摘要： The present disclosure relates to CBH II chimera fusion polypetides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及CBH II嵌合体融合多肽，编码所述多肽的核酸和用于产生所述多肽的宿主细胞。

9. 发明申请

WO2008085900A3 METHODS FOR GENERATING NOVEL STABILIZED PROTEINS 审中-公开
标题翻译：生成新型稳定蛋白的方法
公开(公告)号：WO2008085900A3
公开(公告)日：2008-11-06
申请号：PCT/US2008000135
申请日：2008-01-05
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , LI YOUGEN
发明人： ARNOLD FRANCES H , LI YOUGEN
IPC分类号： C12N9/00 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00 , C12N1/20 , C12N15/00 , C12P21/04 , C12P21/06 , C12Q1/00 , C12Q1/34 , C12Q1/68 , G06F19/16 , G06F19/22
CPC分类号： G06F19/16 , C12N9/0077 , G06F19/22
摘要： The disclosure provides methods for identifying and producing stabilized chimeric proteins.
摘要翻译：本公开提供了用于鉴定和产生稳定的嵌合蛋白的方法。

10. 发明申请

WO2008016709A3 METHODS AND SYSTEMS FOR SELECTIVE FLUORINATION OF ORGANIC MOLECULES 审中-公开
标题翻译：有机分子选择性荧光的方法和系统
公开(公告)号：WO2008016709A3
公开(公告)日：2008-03-27
申请号：PCT/US2007017409
申请日：2007-08-04
申请人： CALIFORNIA INST OF TECHN , FASAN RUDI , ARNOLD FRANCES H
发明人： FASAN RUDI , ARNOLD FRANCES H
IPC分类号： C12P5/02 , C12P1/00
CPC分类号： C07B39/00 , C07C17/16 , C07C45/63 , C07C49/687 , C07H5/02 , C12P7/00 , C12P7/42 , C12P7/62 , C07C23/20
摘要： A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.
摘要翻译：在目标部位选择性氟化有机分子的方法和系统，其中靶位点被活化然后被氟化，以及用于鉴定具有生物活性的分子的方法和系统。

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