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1. 发明申请

WO03008563A9 IMPROVED CYTOCHROME P450 OXYGENASES 审中-公开
标题翻译：改进的CYTOCHROME P450氧化酶
公开(公告)号：WO03008563A9
公开(公告)日：2003-08-14
申请号：PCT/US0223340
申请日：2002-07-22
申请人： CALIFORNIA INST OF TECHN , FARINAS EDGARDO T , GLIEDER ANTON , ARNOLD FRANCES H , SCHWANEBERG ULRICH
发明人： FARINAS EDGARDO T , GLIEDER ANTON , ARNOLD FRANCES H , SCHWANEBERG ULRICH
IPC分类号： C12N9/02 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , C12N
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/16 , C12P17/02 , Y02E50/10 , Y02P20/52
摘要： Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.
摘要翻译：提供了编码细胞色素P450变体的核酸。与相应的野生型或亲本细胞色素P450酶相比，细胞色素P450变体具有更高的烷烃氧化能力，烯烃氧化能力和/或更高的有机溶剂抗性。优选的野生型细胞色素P450是细胞色素P450 BM-3。优选的细胞色素P450变体包括具有改进的包含少于8个碳的烷烃和环氧化物烯烃的羟化能力并且具有对应于细胞色素P450 BM-3的V78A，H236Q和E252G的氨基酸取代的那些。优选的细胞色素P450变体还包括在包含共溶剂例如DMSO和THF的溶液中具有改善的羟基化活性并且具有对应于细胞色素P450 BM-3的T235A，R471A，E494K和S1024E的氨基酸取代的那些。

2. 发明申请

WO2012028709A3 NOVEL MONOOXYGENASE VARIANTS 审中-公开
标题翻译：新的MONOOXYGENASE变体
公开(公告)号：WO2012028709A3
公开(公告)日：2012-04-26
申请号：PCT/EP2011065179
申请日：2011-09-02
申请人： BRAIN BIOTECHNOLOGY RES & INFORMATION NETWORK AG , SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
发明人： SCHWANEBERG ULRICH , BLANUSA MILAN , NIEHAUS FRANK , ECK JUERGEN
IPC分类号： C12N9/02
CPC分类号： C12Y106/02004 , C12N9/0042
摘要： The present invention relates to a nucleic acid molecule encoding a polypeptide having cytochrome P450 monooxygenase activity, wherein said polypeptide comprises a reductase domain that deviates by at least one mutation from (a) the reductase domain of cytochrome P450 BM3, wherein the reductase domain of cytochrome P450 BM3 is represented by SEQ ID NO: 1; or (b) a reductase domain having at least 95% sequence identity to SEQ ID NO:1; and wherein said mutation(s) result(s) in an increased cytochrome P450 monooxygenase activity as compared to a polypeptide comprising the reductase domain of SEQ ID NO: 1. The present invention also relates to a vector comprising the nucleic acid molecule of the invention and a host transformed with the vector. Furthermore, the invention relates to a method of producing a polypeptide comprising culturing the host of the invention as well as to a polypeptide encoded by the nucleic acid molecule of the invention or produced by the method of the invention. The present invention further relates to the use of the polypeptide of the invention in biotransformation or fine chemical synthesis, to an oligo- or polynucleotide which specifically hybridizes to the nucleic acid molecule of the invention as well as to a composition and to a kit.
摘要翻译：本发明涉及编码具有细胞色素P450单加氧酶活性的多肽的核酸分子，其中所述多肽包含与（a）细胞色素P450 BM3的还原酶结构域至少一个突变偏差的还原酶结构域，其中细胞色素的还原酶结构域 P450 BM3由SEQ ID NO：1表示; 或（b）与SEQ ID NO：1具有至少95％序列同一性的还原酶结构域; 并且其中与包含SEQ ID NO：1的还原酶结构域的多肽相比，所述突变导致细胞色素P450单加氧酶活性增加。本发明还涉及包含本发明的核酸分子的载体和用载体转化的宿主。此外，本发明涉及生产多肽的方法，其包括培养本发明的宿主以及由本发明的核酸分子编码的多肽或通过本发明的方法产生的多肽。本发明进一步涉及本发明的多肽在生物转化或精细化学合成中的用途，涉及与本发明的核酸分子以及组合物和试剂盒特异性杂交的寡核苷酸或多核苷酸。

3. 发明申请

WO2013037609A3 METHOD FOR ADAPTING A HYDROLYTIC ENZYME TO A COMPONENT THAT STABILIZES THE HYDROLYTIC ENZYME 审中-公开
标题翻译：将水解酶适配到水解酶稳定化组分的方法
公开(公告)号：WO2013037609A3
公开(公告)日：2013-05-30
申请号：PCT/EP2012066237
申请日：2012-08-21
申请人： HENKEL AG & CO KGAA , SIEGERT PETRA , EVERS STEFAN , MERKEL MARION , MUSMANN NINA , HELLMUTH HENDRIK , O'CONNELL TIMOTHY , MAURER KARL-HEINZ , SCHWANEBERG ULRICH , JAKOB FELIX , MARTINEZ RONNY , LAUFS BRIAN , AYDEMIR AYHAN , WEBER THOMAS
发明人： SIEGERT PETRA , EVERS STEFAN , MERKEL MARION , MUSMANN NINA , HELLMUTH HENDRIK , O'CONNELL TIMOTHY , MAURER KARL-HEINZ , SCHWANEBERG ULRICH , JAKOB FELIX , MARTINEZ RONNY , LAUFS BRIAN , AYDEMIR AYHAN , WEBER THOMAS
IPC分类号： C11D3/386 , C12N9/54
CPC分类号： C12Q1/37 , C11D3/38663 , C11D3/38681 , C12N9/54 , C12N9/96
摘要： The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.
摘要翻译：液态制剂中水解酶的稳定化可通过水解酶稳定组分来改善。这是通过一种用于适配水解酶来水解酶稳定化组分，其包括以下步骤实现：a）提供水解酶（亲本酶）和包含水解酶的可逆抑制剂的水解酶稳定组分; b）通过取代，缺失或插入在至少一个位置改变水解酶的氨基酸序列; c）测定步骤b）的水解酶的相对活性和起始酶在液体制剂中的相对活性; d）选择与亲本酶的相对活性相比具有降低的相对活性的水解酶。

4. 发明申请

WO0107573A8 ELECTRON DONOR SYSTEM FOR ENZYMES AND ITS USE FOR THE BIOCHEMICAL CONVERSION OF SUBSTRATES 审中-公开
标题翻译：电子供体酶的结构和用法基板的生化实现
公开(公告)号：WO0107573A8
公开(公告)日：2001-04-05
申请号：PCT/EP0007251
申请日：2000-07-27
申请人： BASF AG , HAUER BERNHARD , SCHMID ROLF D , SCHWANEBERG ULRICH
发明人： HAUER BERNHARD , SCHMID ROLF D , SCHWANEBERG ULRICH
IPC分类号： C12M1/40 , C12N1/21 , C12N9/02 , C12N15/09 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/11 , C12R1/19 , C12N15/53 , C12N11/06 , C12N11/14
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0077 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The invention relates to a novel electron donor system for enzymes with redox properties and to its use in enzyme-catalyzed oxidation reactions, such as the production of omega hydroxylated fatty acids. The invention further relates to an improved analysis method for fatty acid monoxygenases and to bioreactors and test kits wherein the inventive electron donor system is advantageously used.
摘要翻译：本发明涉及一种用于与氧化还原性质的酶的新型电子给体系统，其在酶催化的氧化反应，如在特定的产生ω羟基化脂肪酸的用途。本发明还涉及一种用于Fettsäuremonoxygenasen，生物反应器和检验试剂盒的改进的检测方法，其中电子给体是有利的。

5. 发明申请

WO0107574A2 MODIFIED CYTOCHROME P450 MONOOXYGENASES 审中-公开
标题翻译：修饰的CYTOCHROM P450 MONOOXYGENASES
公开(公告)号：WO0107574A2
公开(公告)日：2001-02-01
申请号：PCT/EP0007252
申请日：2000-07-27
申请人： BASF AG , HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING SHAN
发明人： HAUER BERNHARD , PLEISS JUERGEN , SCHWANEBERG ULRICH , SCHMITT JUTTA , FISCHER MARKUS , SCHMID ROLF , LI QING-SHAN
IPC分类号： C12M1/40 , C12N1/21 , C12N9/02 , C12N15/09 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P7/64 , C12P17/10 , C12P17/16 , C12Q1/26 , C12R1/11 , C12R1/19 , C12N9/00
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0077 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The invention relates to modified cytochrome P450 monooxygenases comprising a modified substrate profile, to nucleic acid sequences that code therefor, to expression constructs and vectors, and to recombinant microorganisms which contain these vectors. The invention also relates to methods for microbiologically producing terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要翻译：本发明涉及具有改变的底物轮廓，编码核酸序列，表达构建体和载体，含有用于微生物生产终端或近hydroylierter脂族羧酸，这些载体和方法重组微生物修饰细胞色素P450单加氧酶。

6. 发明申请

WO2010070086A2 METHOD FOR THE ENZYMATIC REACTION OF ALKANES 审中-公开
标题翻译： ALKANES的酶法实施过程
公开(公告)号：WO2010070086A2
公开(公告)日：2010-06-24
申请号：PCT/EP2009067493
申请日：2009-12-18
申请人： EVONIK DEGUSSA GMBH , GROEGER HARALD , BURDA EDYTA , SCHWANEBERG ULRICH , MARIENHAGEN JAN , DRAUZ KARLHEINZ
发明人： GROEGER HARALD , BURDA EDYTA , SCHWANEBERG ULRICH , MARIENHAGEN JAN , DRAUZ KARLHEINZ
IPC分类号： C12P7/02 , C12N9/02 , C12N9/04 , C12N15/53 , C12P7/26
CPC分类号： C12P7/02 , C12N9/0006 , C12N9/0071 , C12P7/26
摘要： The invention relates to a method for producing ketones by the enzymatic oxidation of alkanes, in which these alkanes are reacted in the presence of a monooxygenase, an alcohol deydrogenase and a cofactor suitable for both enzymes in an aqueous reaction medium in the presence of molecular oxygen.
摘要翻译：本发明涉及一种通过烷烃的酶促氧化，该方法包括在单加氧酶，醇脱氢酶的存在和用于在分子氧的存在下在水性反应介质中两种酶的合适的辅因子之一这些烷烃反应制备酮的方法。

7. 发明申请

WO2009013115A2 FUNCTIONALIZED NANOCOMPARTMENTS WITH A TRANSPORT SYSTEM 审中-公开
标题翻译：功能齐全的运输系统的纳米技术
公开(公告)号：WO2009013115A2
公开(公告)日：2009-01-29
申请号：PCT/EP2008058735
申请日：2008-07-07
申请人： BASF SE , SCHWANEBERG ULRICH
发明人： SCHWANEBERG ULRICH
CPC分类号： A61K9/1273 , A61K47/6911 , A61K47/6919 , B82Y5/00 , Y10T428/2984
摘要： The invention is directed to vesicles comprising at least one transmembrane triggered transport system for controlled transmembrane efflux and/or influx.
摘要翻译：本发明涉及包含至少一个跨膜触发的转运系统用于受控跨膜流出和/或流入的囊泡。

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