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1. 发明申请

WO0130998A9 PRODUCTION OF FUNCTIONAL HYBRID GENES AND PROTEINS 审中-公开
标题翻译：功能性杂交基因和蛋白质的生产
公开(公告)号：WO0130998A9
公开(公告)日：2002-05-30
申请号：PCT/US0029717
申请日：2000-10-27
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES , SIEBER VOLKER , ZHANG JI HU
发明人： ARNOLD FRANCES , SIEBER VOLKER , ZHANG JI HU
IPC分类号： C07K14/435 , C12N9/02 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1093 , C07K14/43595 , C12N9/0071 , C12N9/0077 , C12N15/1027
摘要： The invention relates to an improved method for creating gene and protein libraries, particularly random gene libraries encoding for hybrid proteins containing fragments from one or two parent proteins. The method may be used to make libraries of circularly permuted variants of genes encoding a single protein or hybrid proteins, especially single chain proteins. In addition, the invention can be used to make libraries for protein fragment complementation, in which the two fragments originate from one parent protein or from two different parent proteins. The method can produce a library of genes mostly of the correct size, leading to a high fraction of functional hybrids or complements. When coupled with suitable screening or selection, the method can be used to create and identify hybrid proteins, including new proteins with new or altered properties. The invention also provides libraries of hybrid proteins, especially single-chain proteins, that include an N-terminal sequence originating from one parent protein, fused to a C-terminal sequence of a second parent protein, with both sequences varying in length among the hybrids of the library.
摘要翻译：本发明涉及用于产生基因和蛋白质文库的改进方法，特别是编码含有一个或两个亲本蛋白质片段的杂交蛋白质的随机基因库。该方法可用于制备编码单一蛋白或杂交蛋白，特别是单链蛋白的基因的循环置换变体的文库。此外，本发明可用于制备用于蛋白质片段互补的文库，其中两个片段源自一个亲本蛋白质或源自两种不同的亲本蛋白质。该方法可以产生大多数正确大小的基因文库，导致功能杂种或补体的高分子量。当与合适的筛选或选择相结合时，该方法可用于产生和鉴定杂合蛋白，包括具有新的或改变的特性的新蛋白质。本发明还提供杂交蛋白，特别是单链蛋白的文库，其包含与来自第一亲本蛋白的C末端序列融合的一个亲本蛋白的N末端序列，两个序列在杂交体之间长度不同的图书馆。

2. 发明申请

WO2010089171A3 METHOD FOR THE AMINATION OF POLYCYCLIC COMPOUNDS USING TRANSAMINASES 审中-公开
标题翻译：多环化合物的方法胺化使用转氨酶
公开(公告)号：WO2010089171A3
公开(公告)日：2011-06-30
申请号：PCT/EP2010050331
申请日：2010-01-13
申请人： EVONIK DEGUSSA GMBH , SIEBER VOLKER , GRAMMANN KATRIN , RUEHMANN BRODER , HAAS THOMAS , PFEFFER JAN CHRISTOPH , DODERER KAI , ROLLMANN CLAUDIA , SKERRA ARNE , RAUSCH CHRISTIAN , LERCHNER ALEXANDRA
发明人： SIEBER VOLKER , GRAMMANN KATRIN , RUEHMANN BRODER , HAAS THOMAS , PFEFFER JAN CHRISTOPH , DODERER KAI , ROLLMANN CLAUDIA , SKERRA ARNE , RAUSCH CHRISTIAN , LERCHNER ALEXANDRA
IPC分类号： C12P17/18 , C07D493/04 , C12P13/00
CPC分类号： C07D493/04 , C12P13/001 , C12P17/181
摘要： The invention relates to a method for the amination of at least one keto group in a multicyclic ring system comprising at least one keto group into an amino group, using at least one enzyme E having transaminase activity.
摘要翻译：本发明描述了至少一个具有多环体系，与转氨酶活性使用的氨基的至少一种酶E的酮基中的至少一个酮基的胺化的方法。

3. 发明申请

WO2005024036A3 METHOD FOR THE ENZYMATIC PRODUCTION OF EMULSIFIERS CONTAINING MONO- AND DIACYLGLYCERIDES 审中-公开
标题翻译： METHOD FOR THE酶法生产MONO和二酰基甘油酯含乳化剂
公开(公告)号：WO2005024036A3
公开(公告)日：2005-11-24
申请号：PCT/EP2004009800
申请日：2004-09-02
申请人： SATIA GMBH , VOLLAND MICHAEL , LOETZBEYER THOMAS , SIEBER VOLKER , WITTMANN EVA
发明人： VOLLAND MICHAEL , LOETZBEYER THOMAS , SIEBER VOLKER , WITTMANN EVA
IPC分类号： A23L29/10 , C12P7/00 , C12P7/64
CPC分类号： C12P7/00 , C12P7/6454
摘要： The invention relates to a method for the enzymatic production of emulsifiers containing mono- and diacylglycerides. In a first step a), a mixture of a phospholipid component and a triacylglyceride component is prepared, then in step b) a quantity of an aqueous solution containing a (phospho)lipase is added to the mixture obtained, to produce a water content of the mixture of between 3 and 15 wt. %. In a subsequent step c), the mixture obtained in the previous step is reacted at a temperature ranging between 20 DEG and 80 DEG C over a period of at least two hours and said mixture is then dried. Crude soy lecithin and vegetable and/or animal oils, which are reacted together with a mixture of lipase and/or phospholipase as the enzymatic component, are particularly suitable as components. Said method, which does not require the usual organic solvents, polyols or ionic additives and which only requires a small quantity of water, permits the production of emulsifiers, whose composition of lysophospholipids and mono-/diacylglycerides can be adjusted in a targeted manner. Said emulsifiers, which can also be obtained in liquid form, are used in the foodstuff and cosmetic industries.
摘要翻译：在用于酶法生产单和含有乳化剂在第一工艺步骤a）磷脂和三酰基甘油酯成分的混合物二酰基的描述过程最初被引入，则在工艺步骤b）至所得的混合物的量（磷酸）含脂肪酶的水溶液鉴于混合物3中的水含量至15重量％（重量）。），来自前面步骤在20“和80'C之间的温度下历时至少两小时得到的混合物，然后在步骤c和反应最后将混合物干燥。特别合适的组分粗大豆卵磷脂和植物油和/或动物油已经显示出与脂肪酶和/或磷脂酶作为酶成分的混合物一起实现。使用这种方法，不需要通常的有机溶剂，多元醇或离子添加剂，并用低水含量，获得乳化剂，该组合物可以从溶血磷脂被调节和单 - /二酰基靶向。您的投注找到这些乳化剂，其也以液体形式提供，在食品和化妆品行业。

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