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1. 发明申请

WO2019147865A1 A METHOD FOR ENANTIOSELECTIVE CARBENE C-H INSERTION USING AN IRON-CONTAINING PROTEIN CATALYST 审中-公开
公开(公告)号：WO2019147865A1
公开(公告)日：2019-08-01
申请号：PCT/US2019/015027
申请日：2019-01-24
申请人： ZHANG, Ruijie , WOHLSCHLAGER, Lena , RENATA, Hans , ARNOLD, Frances H. , CHEN, Kai , CALIFORNIA INSTITUTE OF TECHNOLOGY
发明人： ZHANG, Ruijie , WOHLSCHLAGER, Lena , RENATA, Hans , ARNOLD, Frances H. , CHEN, Kai
IPC分类号： C12P17/18
摘要： Methods for catalyzing C-H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C-H insertion product comprising providing an substrate having an sp 3 -hybridized C-H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C-H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C-H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.

2. 发明申请

WO2011153276A3 STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASE CLASS I ENZYMES 审中-公开
标题翻译：稳定的，功能性的二氧化碳氢化酶I级酶
公开(公告)号：WO2011153276A3
公开(公告)日：2012-04-12
申请号：PCT/US2011038812
申请日：2011-06-01
申请人： CALIFORNIA INST OF TECHN , ARNOLD FRANCES H , HEINZELMAN PETE
发明人： ARNOLD FRANCES H , HEINZELMAN PETE
IPC分类号： C07K19/00 , C12N15/55 , C12N15/62 , C12P19/04
CPC分类号： C12P19/14 , C07K2319/35 , C12N9/2437 , C12P19/02 , C12Y302/01004 , C12Y302/01074 , C12Y302/01091
摘要： The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及CBH I嵌合融合多肽，编码多肽的核酸和用于产生多肽的宿主细胞。

3. 发明申请

WO2010118058A2 POLYPEPTIDES HAVING CELLULASE ACTIVITY 审中-公开
标题翻译：具有纤维素酶活性的多肽
公开(公告)号：WO2010118058A2
公开(公告)日：2010-10-14
申请号：PCT/US2010/030133
申请日：2010-04-06
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , ARNOLD, Frances, H. , HEINZELMAN, Pete
发明人： ARNOLD, Frances, H. , HEINZELMAN, Pete
IPC分类号： C12N15/63 , C12N15/56 , C12N9/42 , C12N15/62 , C07K19/00 , C12P19/04
CPC分类号： C12N9/2437 , C07K2319/00 , C12P19/14 , C12Y302/01004 , C12Y302/01091
摘要： The present disclosure relates to CBH II chimera fusion polypetides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
摘要翻译：本公开涉及CBH II嵌合体融合多肽，编码多肽的核酸和用于产生多肽的宿主细胞。

4. 发明申请

WO2008121435A2 METHODS FOR GENERATING NOVEL STABILIZED PROTEINS 审中-公开
公开(公告)号：WO2008121435A2
公开(公告)日：2008-10-09
申请号：PCT/US2008/052795
申请日：2008-02-01
申请人： THE CALIFORNIA INSTITUTE OF TECHNOLOGY , ARNOLD, Frances, H. , LI, Yougen
发明人： ARNOLD, Frances, H. , LI, Yougen
IPC分类号： C07K14/00 , C12N15/11 , C12N15/00
CPC分类号： C12N9/0079 , C12N9/0042
摘要： The disclosure provides methods for identifying and producing stabilized chimeric proteins.

5. 发明申请

WO2008118545A2 METHODS FOR GENERATING NOVEL STABILIZED PROTEINS 审中-公开
公开(公告)号：WO2008118545A2
公开(公告)日：2008-10-02
申请号：PCT/US2008/053344
申请日：2008-02-07
申请人： THE CALIFORNIA INSTITUTE OF TECHNOLOGY , ARNOLD, Frances, H. , LI, Yougen
发明人： ARNOLD, Frances, H. , LI, Yougen
IPC分类号： C07K14/00 , C12N15/11 , C12N15/00 , C12N5/06 , C12N9/00
CPC分类号： C12N9/0077
摘要： The disclosure provides methods for identifying and producing stabilized chimeric proteins.

6. 发明申请

WO2008016709A9 METHODS AND SYSTEMS FOR SELECTIVE FLUORINATION OF ORGANIC MOLECULES 审中-公开
标题翻译：用于选择性氟化有机分子的方法和系统
公开(公告)号：WO2008016709A9
公开(公告)日：2008-05-29
申请号：PCT/US2007017409
申请日：2007-08-04
申请人： CALIFORNIA INST OF TECHN , FASAN RUDI , ARNOLD FRANCES H
发明人： FASAN RUDI , ARNOLD FRANCES H
IPC分类号： C12P5/02 , C12P1/00
CPC分类号： C07B39/00 , C07C17/16 , C07C45/63 , C07C49/687 , C07H5/02 , C12P7/00 , C12P7/42 , C12P7/62 , C07C23/20
摘要： A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.
摘要翻译：用于选择性氟化靶位点上的有机分子的方法和系统，其中靶位点被活化然后氟化，连同用于鉴定具有生物活性的分子的方法和系统一起显示。

7. 发明申请

WO2005017106A3 LIBRARIES OF OPTIMIZED CYTOCHROME P450 ENZYMES AND THE OPTIMIZED P450 ENZYMES 审中-公开
公开(公告)号：WO2005017106A3
公开(公告)日：2005-02-24
申请号：PCT/US2004/018835
申请日：2004-06-15
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , ARNOLD, Frances H. , OTEY, Christopher R.
发明人： ARNOLD, Frances H. , OTEY, Christopher R.
IPC分类号： C07H21/00
摘要： The present disclosure teaches that the recombination of homologous sequences of P450 enzymes, with the aid of SCHEMA to predict a resulting protein structure, is able to generate libraries of chimeras with significant functional diversity. Additionally, the members of these libraries demonstrate superior or unexpected new properties, which correlate with other factors that are observable in the library. Thus, the making of libraries of optimized P450 enzymes, the analysis of libraries to identify an optimized subset, and the optimized chimeras with improved or altered functionalities are all taught in the present disclosure.

8. 发明申请

WO2003072742A3 NOVEL GLUCOSE 6-OXIDASES 审中-公开
公开(公告)号：WO2003072742A3
公开(公告)日：2003-09-04
申请号：PCT/US2003/005655
申请日：2003-02-27
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , ARNOLD, Frances, H. , SUN, Lian-Hong , PETROUNIA, Loanna, P.
发明人： ARNOLD, Frances, H. , SUN, Lian-Hong , PETROUNIA, Loanna, P.
IPC分类号： C07H21/04
摘要： Glucose oxidase enzymes are provided, including novel variants of galactose oxidase enzymes. The polynucleotides that encode these novel variants can be expressed in recombinant host cell expression systems. The novel variant oxidase enzymes are capable of oxidizing compounds towards which wild-type galactose oxidase (e.g. D-galactose:oxygen 6-oxidoreductase, GAO; EC 1.1.3.9) has little or no activity. Preferred galactose oxidase variants are those which that have improved capability to oxidize secondary alcohols and/or D-glucose relative to the wild-type enzyme.

9. 发明申请

WO2003055978A3 GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT 审中-公开
公开(公告)号：WO2003055978A3
公开(公告)日：2003-07-10
申请号：PCT/US2002/034374
申请日：2002-10-25
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , VOIGT, Christopher, A. , WANG, Zhen-Gang , ARNOLD, Frances, H. , MAYO, Stephen, L.
发明人： VOIGT, Christopher, A. , WANG, Zhen-Gang , ARNOLD, Frances, H. , MAYO, Stephen, L.
IPC分类号： G01N33/48
摘要： The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.

10. 发明申请

WO2003055978A2 GENE RECOMBINATION AND HYBRID PROTEIN DEVELOPMENT 审中-公开
标题翻译：基因重组和杂交蛋白质的开发
公开(公告)号：WO2003055978A2
公开(公告)日：2003-07-10
申请号：PCT/US2002/034374
申请日：2002-10-25
申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY , VOIGT, Christopher, A. , WANG, Zhen-Gang , ARNOLD, Frances, H. , MAYO, Stephen, L.
发明人： VOIGT, Christopher, A. , WANG, Zhen-Gang , ARNOLD, Frances, H. , MAYO, Stephen, L.
IPC分类号： C12N
CPC分类号： G06F19/14 , C12N15/1027 , G06F19/22
摘要： The invention relates to improved methods for directed evolution of polymers, including directed evolution of nucleic acids and proteins. Specifically, the methods of the invention include analytical methods for identifying "crossover locations" in a polymer. Crossovers at these locations are less likely to disrupt desirable properties of the protein, such as stability or functionality. The invention further provides improved methods for directed evolution wherein the polymer is selectively recombined at the identified "crossover locations". Crossover disruption profiles can be used to identify preferred crossover locations. Structural domains of a biopolymer can also be identified and analyzed, and domains can be organized into schema. Schema disruption profiles can be calculated, for example based on conformational energy or interatomic distances, and these can be used to identify preferred or candidate crossover locations. Computer systems for implementing analytical methods of the invention are also provided.
摘要翻译：本发明涉及聚合物定向进化的改进方法，包括核酸和蛋白质的定向进化。具体地，本发明的方法包括用于识别聚合物中的“交叉位置”的分析方法。这些位置处的交叉链不太可能破坏蛋白质的所需性质，例如稳定性或功能性。本发明还提供了用于定向进化的改进方法，其中聚合物在所识别的“交叉位置”选择性重组。交叉中断配置文件可用于标识首选交叉位置。还可以鉴定和分析生物聚合物的结构域，并将结构域组织成模式。可以例如基于构象能量或原子间距离来计算模式中断简档，并且这些可以用于识别优选或候选交叉位置。还提供了用于实现本发明的分析方法的计算机系统。

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