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2. 发明申请

WO1994001578A1 STABILIZING TETRAZOLIUM SALTS IN A REAGENT 审中-公开
标题翻译：在试剂中稳定四唑盐
公开(公告)号：WO1994001578A1
公开(公告)日：1994-01-20
申请号：PCT/US1993006284
申请日：1993-07-01
申请人： BOEHRINGER MANNHEIM CORPORATION
发明人： BOEHRINGER MANNHEIM CORPORATION , STORHOFF, Diana, Faye , TABB, David
IPC分类号： C12Q01/42
CPC分类号： C12Q1/32 , C12Q1/26 , Y10S436/80
摘要： A stable reagent for the assay of an analyte from a fluid sample. The reagent includes an enzyme, a mediator, a tetrazolium salt and an oxidizing agent selected from the group consisting of sodium chlorate, 2,5-dimethylhexane-2,5-dihydroperoxide, benzoyl peroxide, t-butyl peroxide, sodium iodate, N-ethylmaleimide, t-butylperoxyacetate, nickel acetylacetonate, stannic chloride, rhodium (III) trichloride hydrate, and t-butylperbenzoate. Importantly, inclusion of an oxidizing agent from the group specified above stabilizes the reagent, thereby preventing a high blank reaction (which introduces error into the assay of an analyte). The reagent may be provided in solution form by adding water, or may be included in a film by adding a film-forming agent. The reagent may be utilized to perform the assay of an analyte from a fluid sample. The assay may be conducted in a solution medium by adding the fluid sample to the liquid reagent, and spectrophotometrically measuring absorbance of the color indicating formazan (produced by reduction of the tetrazolium salt under assay conditions). Likewise, the assay may be conducted in a film medium by adding the fluid sample to the reagent incorporated in the film, and spectrophotometrically measuring reflectance or transmittance of the color indicating formazan.
摘要翻译：用于从流体样品测定分析物的稳定试剂。该试剂包括选自氯酸钠，2,5-二甲基己烷-2,5-二氢过氧化物，过氧化苯甲酰，叔丁基过氧化物，碘酸钠，N-甲基-2-吡咯烷酮，乙基马来酰亚胺，过氧乙酸叔丁酯，乙酰丙酮镍，氯化锡，三氯化铑（III）和叔丁基过苯甲酸酯。重要的是，包含来自上述基团的氧化剂使试剂稳定，从而防止高的空白反应（其导致测定分析物的误差）。试剂可以通过加入水而以溶液形式提供，或者可以通过加入成膜剂包含在膜中。试剂可用于从流体样品中进行分析物的测定。该测定可以在溶液介质中通过将液体样品加入到液体试剂中进行，并且通过分光光度测量法来测量指示甲the（通过在测定条件下还原四唑鎓盐产生的）的颜色的吸光度。同样地，该测定法可以通过将流体样品加入到膜中的试剂中并在分光光度法测量指示甲the的颜色的反射率或透射率的情况下在膜介质中进行。

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