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    • 9. 发明申请
    • SYSTEM AND METHOD FOR DIAGNOSING DISEASES
    • 用于诊断疾病的系统和方法
    • WO2009045552A1
    • 2009-04-09
    • PCT/US2008/011558
    • 2008-10-03
    • BECTON, DICKINSON AND COMPANYGELFAND, Craig, A.YI, JizuJU, Gang
    • GELFAND, Craig, A.YI, JizuJU, Gang
    • G01N33/68G01N33/86
    • G01N33/68G01N33/86G01N2333/75
    • One aspect of the invention provides a method of diagnosing a disease condition, comprising measuring presence or amount of a targeted protein or a degradation product of said protein in a collected biological sample as a marker for the disease condition. The targeted protein or degradation product is selected for measurement based on a prior identification of a measurable half-life at a predetermined time period, including the time at which said method is conducted, and correlating said measuring with the presence or absence of the disease condition. The targeted protein or degradation product may be identified by selecting a protein known or suspected to be a diagnostic marker for the disease condition, analyzing degradation of the protein in the collected biological sample, and selecting a protein or degradation product that exhibits a measurable half-life at a predetermined period of time. The analyzing may include identifying degradation product (s) of the protein as a function of time, and half-life of the protein and the degradation product (s).
    • 本发明的一个方面提供了一种诊断疾病状况的方法,包括测定收集的生物样品中所述蛋白质的靶向蛋白质或降解产物的存在或量作为疾病状况的标志物。 基于在预定时间段(包括进行所述方法的时间)的可测量的半衰期的先前识别,并将所述测量与存在或不存在疾病状态相关联,选择靶向蛋白质或降解产物进行测量 。 可以通过选择已知或怀疑是疾病状况的诊断标记物的蛋白质或分解产物来分析靶向蛋白质或降解产物,分析所收集的生物样品中蛋白质的降解,以及选择显示可测量的半衰期的蛋白质或降解产物, 寿命在预定的时间。 分析可以包括鉴定蛋白质的降解产物作为时间的函数,以及蛋白质和降解产物的半衰期。