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1. 发明申请

WO2010141940A1 RECOMBINASE POLYMERASE AMPLIFICATION REAGENTS AND KITS 审中-公开
标题翻译：重组聚合酶扩增试剂和试剂盒
公开(公告)号：WO2010141940A1
公开(公告)日：2010-12-09
申请号：PCT/US2010/037611
申请日：2010-06-07
申请人： ALERE SAN DIEGO, INC. , PIEPENBURG, Olaf , ARMES, Niall, A.
发明人： PIEPENBURG, Olaf , ARMES, Niall, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q2549/101 , C12Q2527/125 , C12Q2521/507 , C12Q2527/137
摘要： This disclosure describes kits, reagents and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed kits, reagents and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods.
摘要翻译：本公开描述了利用重组酶和相关蛋白质的特性的靶DNA的重组酶聚合酶扩增（RPA）的试剂盒，试剂和方法，以允许DNA聚合酶反应的序列特异性引发的单链同源DNA侵入双链DNA。所公开的试剂盒，试剂和方法具有不需要热循环或嗜热酶的优点，因此相对于其它扩增方法提供了容易且价格实惠的实施和便携性。

2. 发明申请

WO2011038197A1 DETECTION OF NUCLEIC ACIDS IN CRUDE MATRICES 审中-公开
标题翻译：在原子核中检测核酸
公开(公告)号：WO2011038197A1
公开(公告)日：2011-03-31
申请号：PCT/US2010/050151
申请日：2010-09-24
申请人： ALERE SAN DIEGO, INC. , ARMES, Niall, A.
发明人： ARMES, Niall, A.
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , Y02A50/53 , C12Q2527/101
摘要： A method includes contacting a crude matrix with components of an isothermal nucleic acid amplification reaction for a target nucleic acid species, thereby providing a mixture; incubating the mixture under conditions sufficient for the isothermal nucleic acid amplification reaction to proceed, thereby providing a product; and determining whether an indicator of the target nucleic acid species is present in the product.
摘要翻译：一种方法包括使粗制基质与靶核酸物质的等温核酸扩增反应的组分接触，从而提供混合物; 在足以进行等温核酸扩增反应的条件下孵育混合物，从而提供产物; 以及确定产物中是否存在目标核酸物种的指示物。

3. 发明申请

WO2010135310A8 DNA GLYCOSYLASE/LYASE AND AP ENDONUCLEASE SUBSTRATES 审中-公开
公开(公告)号：WO2010135310A8
公开(公告)日：2010-11-25
申请号：PCT/US2010/035232
申请日：2010-05-18
申请人： BIOSITE INCORPORATED , PIEPENBURG, Olaf , ARMES, Niall, A.
发明人： PIEPENBURG, Olaf , ARMES, Niall, A.
IPC分类号： C07H21/04 , C12Q1/68
摘要： A new class of nucleic acid substrates for AP endonucleases and members of the glycosylase/lyase family of enzymes is described. Representatives of each family, the enzymes Nfo and fpg, respectively, cleave nucleic acid backbones at positions in which a base has been replaced by a linker to which a variety of label moieties may be attached. The use of these synthetic substrates embedded within oligonucleotides is of utility in a number of applications.

4. 发明申请

WO2005118853A2 RECOMBINASE POLYMERASE AMPLIFICATION 审中-公开
标题翻译：重组聚合酶扩增
公开(公告)号：WO2005118853A2
公开(公告)日：2005-12-15
申请号：PCT/IB2005/001560
申请日：2005-04-11
申请人： ASM SCIENTIFIC, INC. , PIEPENBURG, Olaf , WILLIAMS, Colin, H. , ARMES, Niall, A. , STEMPLE, Derek, L.
发明人： PIEPENBURG, Olaf , WILLIAMS, Colin, H. , ARMES, Niall, A. , STEMPLE, Derek, L.
IPC分类号： C12Q1/68
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6827 , C12Q1/6844 , C12Q2521/507 , C12Q2522/101 , C12Q2527/125 , C12Q2531/119
摘要： This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carryover contamination, and methods to employ sequence-specific third `specificity' probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments.
摘要翻译：本公开描述了利用重组酶和相关蛋白质的特性的靶DNA的重组酶 - 聚合酶扩增（RPA）的相关新方法，以允许DNA聚合酶反应的序列特异性引发的单链同源DNA侵入双链DNA。所公开的方法具有不需要热循环或嗜热酶的优点，因此相对于其它扩增方法提供了容易和可负担的实施和便携性。进一步公开的是能够实时监测RPA反应的条件，使用光调节RPA反应的方法，否则，确定扩增物种的性质而不需要凝胶电泳的方法，改进和优化RPA中的信噪比的方法反应，优化寡核苷酸引物功能的方法，控制携带污染的方法以及使用序列特异性第三“特异性”探针的方法。进一步描述了在动态重组环境中使用由光监测的探针的新颖性质和方法。

5. 发明申请

WO2007096702A2 METHODS FOR MULTIPLEXING RECOMBINASE POLYMERASE AMPLIFICATION 审中-公开
标题翻译：多重复性聚合酶扩增的方法
公开(公告)号：WO2007096702A2
公开(公告)日：2007-08-30
申请号：PCT/IB2006/004113
申请日：2006-07-25
申请人： ASM SCIENTIFIC, INC. , PIEPENBURG, Olaf , WILLIAMS, Colin, H. , ARMES, Niall, A.
发明人： PIEPENBURG, Olaf , WILLIAMS, Colin, H. , ARMES, Niall, A.
CPC分类号： C12N15/113 , C12Q1/6804 , C12Q1/6816 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , G01N21/78 , G01N33/558 , G01N2021/7759 , G01N2021/7763 , G01N2021/7793 , C12Q2521/507 , C12Q2565/102 , C12Q2537/1376 , C12Q2565/101 , C12Q2525/119 , C12Q2521/301 , C12Q2561/113 , C12Q2537/125 , C12Q2525/125 , C12Q2563/131 , C12Q2565/625
摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
摘要翻译：本公开提供了用于快速多重RPA反应的方法和试剂以及用于检测多重RPA反应产物的改进方法。此外，本公开提供了消除RPA过程之间的残留污染的新方法。

6. 发明申请

WO2005118608A2 2’-NITROBENZYL-MODIFIED RIBONUCLEOTIDES 审中-公开
标题翻译： 2-NITROBENZYL修饰的RIBONUCLEOTIDES
公开(公告)号：WO2005118608A2
公开(公告)日：2005-12-15
申请号：PCT/IB2005/002488
申请日：2005-06-02
申请人： ASM SCIENTIFIC, INC. , PIEPENBURG, Olaf , STEMPLE, Derek, L. , ARMES, Niall, A.
发明人： PIEPENBURG, Olaf , STEMPLE, Derek, L. , ARMES, Niall, A.
IPC分类号： C07H
CPC分类号： C07H19/10 , C07H19/04 , C07H19/20
摘要： This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclsoed nucleotides are also provided.
摘要翻译：本公开提供了可用作DNA测序反应试剂的新型可逆终止的核糖核苷酸。还提供了使用转座核苷酸测序核酸的方法。

7. 发明申请

WO2003072805A3 RECOMBINASE POLYMERASE AMPLIFICATION 审中-公开
公开(公告)号：WO2003072805A3
公开(公告)日：2003-09-04
申请号：PCT/US2003/005492
申请日：2003-02-21
申请人： ASM SCIENTIFIC, INC. , ARMES, Niall, A. , STEMPLE, Derek, L.
发明人： ARMES, Niall, A. , STEMPLE, Derek, L.
IPC分类号： C12P19/34
摘要： This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed method has the advantage of not requiring tehrmocycling or thermophilic enzymes. Further, the improved processivity of the dislosed methods allow amplification of DNA up to hundreds of megabases in length.

8. 发明申请

WO2003072805A2 RECOMBINASE POLYMERASE AMPLIFICATION 审中-公开
标题翻译：重组聚合酶扩增
公开(公告)号：WO2003072805A2
公开(公告)日：2003-09-04
申请号：PCT/US2003/005492
申请日：2003-02-21
申请人： ASM SCIENTIFIC, INC. , ARMES, Niall, A. , STEMPLE, Derek, L.
发明人： ARMES, Niall, A. , STEMPLE, Derek, L.
IPC分类号： C12Q
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q1/6848 , C12Q1/686 , G06F17/30864 , G06F17/30902 , C12Q2521/507 , C12Q2522/101 , C12Q2549/119 , C12Q2527/101 , C12Q2531/119
摘要： This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed method has the advantage of not requiring tehrmocycling or thermophilic enzymes. Further, the improved processivity of the dislosed methods allow amplification of DNA up to hundreds of megabases in length.
摘要翻译：本公开内容描述了利用细菌RecA和相关蛋白质性质的目标DNA的重组酶 - 聚合酶扩增（RPA）的三种相关新方法，以单链同源DNA侵入双链DNA，允许DNA聚合酶反应的序列特异性引发。所公开的方法具有不需要tehrmocycling或嗜热酶的优点。此外，解离方法的改进的持续性允许DNA长达几百兆碱基的扩增。

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