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1. 发明申请

WO0018962A9 METHODS AND COMPOSITIONS FOR AMPLIFYING DETECTABLE SIGNALS IN SPECIFIC BINDING ASSAYS 审中-公开
标题翻译：用于在特定结合测定中扩增可检测信号的方法和组合物
公开(公告)号：WO0018962A9
公开(公告)日：2000-08-24
申请号：PCT/US9922584
申请日：1999-09-28
申请人： AFFYMETRIX INC , GOLDBERG MARTIN J , YELAGALAWADI GOVINDA RAO S , TANIMOTO EUGENE YUJI , TRAN HUU MINH , DONG HELIN , LOCKHART DAVID J , RYDER THOMAS B , WARRINGTON JANET A , BEECHER JODY
发明人： GOLDBERG MARTIN J , YELAGALAWADI GOVINDA RAO S , TANIMOTO EUGENE YUJI , TRAN HUU MINH , DONG HELIN , LOCKHART DAVID J , RYDER THOMAS B , WARRINGTON JANET A , BEECHER JODY
IPC分类号： C12N15/09 , C12Q1/68 , G01N37/00 , G01N33/58
CPC分类号： C12Q1/682 , C12Q1/6804 , Y10S436/807 , C12Q2565/501 , C12Q2563/131 , C12Q2527/125 , C12Q2563/107 , C12Q2563/125 , C12Q2563/143 , C12Q2537/125
摘要： Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hybridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of large number of nucleic acids.
摘要翻译：提供了使用特异性结合测定法检测样品中的靶分子的方法和化合物。具体而言，提供了用于检测样品中的核酸靶标的方法。在一个实施方案中，所述方法包括使包含靶核酸序列的核酸靶与包含探针核酸序列的核酸探针杂交，其中所述靶包含结合配体。使杂交的靶与包含能够结合结合配体的多个位点的受体接触以将受体与结合配体复合，并使受体与包含多个结合配体的扩增试剂接触以将扩增试剂复合成受体。然后检测复合的扩增试剂的存在，例如通过检测例如受体或扩增试剂上的可检测标记（例如荧光标记）的存在。任选地，使包含多个结合配体的扩增试剂与标记的受体分子接触，从而将多个标记的受体分子与扩增试剂复合，并检测与扩增试剂复合的标记的受体分子。这允许可检测信号被增强和放大。在一个实施方案中，结合配体是生物素，受体是链霉抗生物素蛋白，扩增试剂是抗体或DNA基质。在另一个实施方案中，固定在表面上的不同核酸探针的阵列，每个核酸探针在表面上都具有限定的序列和位置，可以用于测定中，从而允许筛选和检测大量核酸的结合。

2. 发明申请

WO0066265A9 PROBES FOR A GAS PHASE ION SPECTROMETER 审中-公开
标题翻译：气相离子光谱仪的探针
公开(公告)号：WO0066265A9
公开(公告)日：2001-09-07
申请号：PCT/US0011452
申请日：2000-04-27
申请人： CIPHERGEN BIOSYSTEMS INC , RICH WILLIAM E , UM PIL JE , VOIVODOV KAMEN , YIP TAI TUNG , BEECHER JODY
发明人： RICH WILLIAM E , UM PIL-JE , VOIVODOV KAMEN , YIP TAI-TUNG , BEECHER JODY
IPC分类号： H01J49/16 , C08F20/10 , C08F20/56 , G01N1/28 , G01N27/62 , G01N27/64 , H01J49/04 , H01J49/12
CPC分类号： H01J49/0418 , H01J49/12 , Y10T428/261 , Y10T428/31504 , Y10T436/24
摘要： The invention provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, the hydrogel material comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. The invention also provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a plurality of particles that are uniform in diameter on the surface, the particles comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. Further, the invention provides a system comprising the probe of the present invention and a gas phase ion spectrometer comprising an energy source that directs light to the probe surface to desorb an analyte and a detector in communication with the probe surface that detects the desorbed analyte. The invention also provides a method for desorbing an analyte from a probe surface, the method comprising exposing the binding functionalities to a sample containing an analyte under conditions to allow binding between the analyte and the binding functionalities, and desorbing the analyte from the probe by gas phase ion spectrometry.
摘要翻译：本发明提供一种探针和一种使探针可移除地插入到气相离子光谱仪中的探针，该探针包括在表面上具有表面和水凝胶材料的基底，该水凝胶材料包含用于与分析物结合的结合官能团可由气相离子光谱仪检测。本发明还提供了一种探针和一种使探针可移除地插入到气相离子分光计中的方法，该探针包括具有表面和表面上直径均匀的多个颗粒的基底，该颗粒包含结合用于与气相离子光谱仪可检测的分析物结合的功能。此外，本发明提供了一种包括本发明的探针和气相离子光谱仪的系统，该气相离子光谱仪包括将光引导到探针表面以解吸分析物的能量源和与检测解吸附的分析物的探针表面连通的检测器。本发明还提供了一种用于从探针表面解吸分析物的方法，该方法包括在允许分析物和结合官能团之间结合的条件下将结合功能暴露于含有分析物的样品，并通过气体从探针解吸分析物相离子光谱法。

3. 发明申请

WO0066265A8 PROBES FOR A GAS PHASE ION SPECTROMETER 审中-公开
公开(公告)号：WO0066265A8
公开(公告)日：2001-03-15
申请号：PCT/US0011452
申请日：2000-04-27
申请人： CIPHERGEN BIOSYSTEMS INC , RICH WILLIAM E , UM PIL JE , VOIVODOV KAMEN , YIP TAI TUNG , BEECHER JODY
发明人： RICH WILLIAM E , UM PIL-JE , VOIVODOV KAMEN , YIP TAI-TUNG , BEECHER JODY
IPC分类号： H01J49/16 , C08F20/10 , C08F20/56 , G01N1/28 , G01N27/62 , G01N27/64 , H01J49/04 , H01J49/12 , B01L
CPC分类号： H01J49/0418 , H01J49/12 , Y10T428/261 , Y10T428/31504 , Y10T436/24
摘要： The invention provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a hydrogel material on the surface, the hydrogel material comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. The invention also provides a probe and a method of making the probe that is removably insertable into a gas phase ion spectrometer, the probe comprising a substrate having a surface and a plurality of particles that are uniform in diameter on the surface, the particles comprising binding functionalities for binding with an analyte detectable by the gas phase ion spectrometer. Further, the invention provides a system comprising the probe of the present invention and a gas phase ion spectrometer comprising an energy source that directs light to the probe surface to desorb an analyte and a detector in communication with the probe surface that detects the desorbed analyte. The invention also provides a method for desorbing an analyte from a probe surface, the method comprising exposing the binding functionalities to a sample containing an analyte under conditions to allow binding between the analyte and the binding functionalities, and desorbing the analyte from the probe by gas phase ion spectrometry.

4. 发明申请

WO0067293A8 SAMPLE HOLDER WITH HYDROPHOBIC COATING FOR GAS PHASE MASS SPECTROMETERS 审中-公开
标题翻译：气相色谱仪用HYPOPHOBIC涂料样品夹具
公开(公告)号：WO0067293A8
公开(公告)日：2001-06-28
申请号：PCT/US0011499
申请日：2000-04-27
申请人： CIPHERGEN BIOSYSTEMS INC , BEECHER JODY , SCHEUFELE FRANK , VOIVODOV KAMEN , WEINBERGER SCOT , LANDGRAF WILLIAM C
发明人： BEECHER JODY , SCHEUFELE FRANK , VOIVODOV KAMEN , WEINBERGER SCOT , LANDGRAF WILLIAM C
IPC分类号： G01N27/62 , B01L3/00 , G01N27/64 , G01N35/00 , H01J49/04 , H01J49/10
CPC分类号： H01J49/0418 , B01L3/5085 , Y10S977/881 , Y10S977/891 , Y10S977/892 , Y10T436/255
摘要： This invention provides sample holder for mass spectrometry including a substrate having a surface and a film that coats the surface. The film includes openings that define features for the presentation of an analyte. The film also has a lower surface tension than the surface tension of the substrate surface, and has a water contact angle between 120 DEG and 180 DEG .
摘要翻译：本发明提供了用于质谱的样品架，包括具有表面的基材和涂覆该表面的膜。该膜包括限定用于呈现分析物的特征的开口。该膜的表面张力比基板表面张力低，并且水接触角在120°和180°之间。

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