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1. 发明申请

WO1996033263A1 NOVEL MICROORGANISMS CAPABLE OF PRODUCING HIGHLY UNSATURATED FATTY ACIDS AND PROCESS FOR PRODUCING HIGHLY UNSATURATED FATTY ACIDS BY USING THE MICROORGANISMS 审中-公开
标题翻译：能够生产高度不饱和脂肪酸的新型微生物和使用微生物生产高度不饱和脂肪酸的方法
公开(公告)号：WO1996033263A1
公开(公告)日：1996-10-24
申请号：PCT/JP1996001049
申请日：1996-04-17
申请人： JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY , NAGASE BIOCHEMICALS LTD. , SUNTORY LIMITED , YOKOCHI, Toshihiro , NAKAHARA, Toro , HIGASHIHARA, Takanori , TANAKA, Satohiro , YAGUCHI, Toshiaki
发明人： JAPAN, represented by DIRECTOR-GENERAL OF AGENCY OF INDUSTRIAL SCIENCE AND TECHNOLOGY , NAGASE BIOCHEMICALS LTD. , SUNTORY LIMITED
IPC分类号： C12N01/14
CPC分类号： C12P7/6427 , A23K20/158 , A23K50/75 , A23K50/80 , A23L15/00 , A23L33/12 , C11B1/00 , C11C3/00 , C12P7/6472 , C12R1/645 , Y10S435/911 , Y10S435/946
摘要： A microorganism which is Schizochytrium SR21 strain capable of producing (n-3) docosahexaenoic acid (DHA) and (n-6) docosapentaenoic acid (DPA), one which belongs to the same genus as that of this strain, and one which has substantially the same mycological properties as those of these microorganisms; and a process for producing (n-3) DHA and (n-6) DPA by using these microorganisms. They are excellent in reproduction properties and the capability of accumulating fats and have an extremely high (n-3) DHA and (n-6) DPA productivity. Thus, (n-3) DHA and/or (n-6) DPA, which are useful in the fields of foods and drugs, can be efficiently produced by using these microorganisms. Also, fats obtained by incubating these microorganisms are provided. When added to various feeds or foods, these fat compositions containing (n-3) DHA having various physiological activities together with (n-6) DPA it possible to stably and efficiently supply (n-3) DHA and/or (n-6) DPA to the subjects requiring the same.
摘要翻译：能够产生（n-3）二十二碳六烯酸（DHA）和（n-6）二十二碳五烯酸（DPA）的裂殖酵母SR21菌株属于与该菌株相同属的微生物，其具有基本上与这些微生物相同的真菌学性质; 以及通过使用这些微生物来生产（n-3）DHA和（n-6）DPA的方法。它们具有优异的繁殖性能和积聚脂肪的能力，具有非常高的（n-3）DHA和（n-6）DPA生产率。因此，可以通过使用这些微生物有效地制备可用于食品和药物领域的（n-3）DHA和/或（n-6）DPA。此外，提供通过温育这些微生物获得的脂肪。当添加到各种饲料或食品中时，含有具有各种生理活性（n-6）DPA的（n-3）DHA的这些脂肪组合物可以稳定和有效地供应（n-3）DHA和/或（n-6））DPA给需要相同的科目。

2. 发明申请

WO1986004354A1 METHOD FOR OBTAINING LIPIDS FROM FUNGUS BODIES 审中-公开
标题翻译：从真菌体内获取脂肪的方法
公开(公告)号：WO1986004354A1
公开(公告)日：1986-07-31
申请号：PCT/JP1985000685
申请日：1985-12-13
申请人： JAPAN as represented by DIRECTOR GENERAL OF ... , SUZUKI, Osamu , YOKOCHI, Toshihiro
发明人： JAPAN as represented by DIRECTOR GENERAL OF ...
IPC分类号： C12P07/64
CPC分类号： C12P7/6472 , C11B1/10 , C11B7/0008 , C12N1/005
摘要： Procedure of obtaining a lipid mixture rich in the content of gamma -linolenic acid or glycerides thereof from a cultured fungus bodies of a filamentous Mortierella fungus. The invention proposes, on one hand, to fractionally extract the lipid mixture from the fungus bodies under mechanical disintegration in two steps first with an alcohol containing water to give a fraction rich in the polar lipids and then with a hydrocarbon solvent, e.g. hexane, to give a fraction lean in the polar lipids. The invention proposes, on the other hand, to enrich a lipid mixture relative to gamma -linolenic acid by fractionally crystallizing a part of a staring lipid mixture in a solution of a specified solvent so that the uncrystallized fraction in the mother liquor contains a lipid mixture in which the content of gamma -linolenic acid is remarkably increased in comparison with the starting lipid mixture.
摘要翻译：从丝状被孢霉真菌的培养真菌体获得富含γ-亚麻酸或其甘油酯含量的脂质混合物的方法。本发明一方面提出，首先用含醇的水分两步分离地从机体分解机械分解提取脂质混合物，得到富含极性脂质的馏分，然后用烃溶剂，例如，己烷，使极性脂质中的馏分变薄。另一方面，本发明提出通过将一部分凝胶状脂质混合物在特定溶剂的溶液中分级结晶来富集相对于γ-亚麻酸的脂质混合物，使得母液中的未结晶部分含有脂质混合物其中与起始脂质混合物相比，γ-亚麻酸的含量显着增加。

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