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    • 3. 发明申请
    • FRET-ASSAYS, METHODS FOR PERFORMING THE ASSAYS AND COMPOUNDS RELEVANT TO THE ASSAYS
    • FRET-ASSAYS,用于进行测定的方法和与测定相关的化合物
    • WO2010090756A2
    • 2010-08-12
    • PCT/US2010000341
    • 2010-02-05
    • ANASPEC INCHONG ANITARAKHMANOVA VERATONG XIAO-HE
    • HONG ANITARAKHMANOVA VERATONG XIAO-HE
    • G01N33/542C07K7/06G01N33/582G01N2333/95
    • The present invention is generally directed to biological assays. More specifically it is directed to FRET-based assays using particularly effecting FRET pairs, methods for performing such assays and the molecules utilized in the assays. In a composition aspect, the present invention is directed to a FRET-based protease substrate selected from the list of substrates shown in the Detailed Description section above. In a method aspect, the present invention is directed to a method of performing a FRET-based assay, where the assay includes the following steps: adding a solution or suspension containing one or more different proteins to a reaction vessel; adding a liquid comprising a FRET-substrate to the reaction vessel, wherein the FRET-substrate is selected from the list of substrates shown in the Detailed Description section above. In a kit aspect, the present invention is directed to a kit for performing a FRET-based assay, wherein the kit comprises a protease substrate. The protease substrate is selected from the list of substrates shown in the Detailed Description section above.
    • 本发明一般涉及生物测定。 更具体地,其涉及使用特别有效的FRET对的FRET-测定法,用于进行此类测定的方法和用于测定中的分子。 在组合物方面,本发明涉及选自上述详细描述部分中所示的基材列表的基于FRET的蛋白酶底物。 在方法方面,本发明涉及一种进行基于FRET的测定法,其中测定法包括以下步骤:向反应容器中加入含有一种或多种不同蛋白质的溶液或悬浮液; 向反应容器中加入包含FRET-底物的液体,其中FRET-基材选自上述详细描述部分所示的基材列表。 在试剂盒方面,本发明涉及用于进行基于FRET的测定的试剂盒,其中所述试剂盒包含蛋白酶底物。 蛋白酶底物选自上述详细描述部分中所示的底物列表。