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1. 发明申请

WO2003099216A3 FATTY ACID DESATURASES FROM FUNGI 审中-公开
公开(公告)号：WO2003099216A3
公开(公告)日：2003-12-04
申请号：PCT/US2003/016144
申请日：2003-05-21
申请人： MONSANTO TECHNOLOGY LLC , URSIN, Virginia, M. , VOELKER, Toni , FROMAN, Byron
发明人： URSIN, Virginia, M. , VOELKER, Toni , FROMAN, Byron
IPC分类号： A01H5/10
摘要： The invention relates generally to methods and compositions concerning fungal desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In Particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are fungal -15 desaturases. Also provided are improved canola oil compositions having SDA and maintaining beneficial oleic acid content.

2. 发明申请

WO2004056848A2 ELEVATION OF OIL LEVELS IN BRASSICA PLANTS 审中-公开
标题翻译：油菜种植面积的增加
公开(公告)号：WO2004056848A2
公开(公告)日：2004-07-08
申请号：PCT/US2003/041474
申请日：2003-12-19
申请人： MONSANTO TECHNOLOGY, LLC , VOELKER, Toni, A. , VAL, Dale, L. , SAVAGE, Thomas, J.
发明人： VOELKER, Toni, A. , VAL, Dale, L. , SAVAGE, Thomas, J.
IPC分类号： C07K
CPC分类号： C12N9/1029 , C12N15/8247
摘要： This present invention provides a method for increasing oil levels in the tissues of Brassica sp. plants by expression of a heterologous multifunctional fatty acid synthase (mfFAS) within the plant. In one embodiment, the present invention provides isolated nucleic acid molecules encoding mfFAS enzymes from various sources for this purpose, and vectors and plants containing same.
摘要翻译：本发明提供了一种提高芸苔属组织中油含量的方法。植物通过在植物中表达异源多功能脂肪酸合成酶（mfFAS）。在一个实施方案中，本发明提供从各种来源为此目的编码mfFAS酶的分离的核酸分子，以及含有它们的载体和植物。

3. 发明申请

WO2003099216A2 FATTY ACID DESATURASES FROM FUNGI 审中-公开
标题翻译：来自真菌的脂肪酸
公开(公告)号：WO2003099216A2
公开(公告)日：2003-12-04
申请号：PCT/US2003/016144
申请日：2003-05-21
申请人： MONSANTO TECHNOLOGY LLC , URSIN, Virginia, M. , VOELKER, Toni , FROMAN, Byron
发明人： URSIN, Virginia, M. , VOELKER, Toni , FROMAN, Byron
IPC分类号： A61K
CPC分类号： C12N9/0083 , C12N15/8247
摘要： The invention relates generally to methods and compositions concerning fungal desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In Particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are fungal -15 desaturases. Also provided are improved canola oil compositions having SDA and maintaining beneficial oleic acid content.
摘要翻译：本发明一般涉及调节长链多不饱和脂肪酸（LC-PUFA）中双键的数量和位置的真菌去饱和酶的方法和组合物。特别地，本发明涉及用于使用去饱和酶和编码这些酶的核酸改进植物产品和部分中的ω-3脂肪酸谱的方法和组合物。在具体实施方案中，去饱和酶是真菌-15去饱和酶。还提供了具有SDA的改良的卡诺拉油组合物并保持有益的油酸含量。

4. 发明申请

WO1996023892A3 PRODUCTION OF MYRISTATE IN PLANT CELLS 审中-公开
公开(公告)号：WO1996023892A3
公开(公告)日：1996-08-08
申请号：PCT/US1996001585
申请日：1996-02-01
申请人： CALGENE, INC. , DEHESH, Katayoon , VOELKER, Toni, Alois , HAWKINS, Deborah
发明人： CALGENE, INC.
IPC分类号： C12N15/82

5. 发明申请

WO1995013390A2 PLANT ACYL ACP THIOESTERASE SEQUENCES 审中-公开
标题翻译：植物ACYL ACP硫酸酯酶序列
公开(公告)号：WO1995013390A2
公开(公告)日：1995-05-18
申请号：PCT/US1994013131
申请日：1994-11-10
申请人： CALGENE, INC. , VOELKER, Toni, Alois , YUAN, Ling , KRIDL, Jean , HAWKINS, Deborah , JONES, Aubrey
发明人： CALGENE, INC.
IPC分类号： C12N15/82
CPC分类号： A23D9/00 , C12N9/16 , C12N15/8247
摘要： This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.
摘要翻译：本发明涉及植物硫酯酶，特别是具有对棕榈酰-ACP底物具有显着活性的植物酰基-ACP硫酯酶。描述了可用于在植物种子细胞中表达植物棕榈酰-ACP硫酯酶的DNA构建体。与其他植物组织相比，这样的构建体将含有编码植物棕榈酰-ACP硫酯酶的DNA序列，该调控元件能够优先指导植物棕榈酰-ACP硫酯酶在种子组织中的表达，在转基因植物中表达。本发明还涉及使用编码植物棕榈酰-ACP硫酯酶的DNA序列来修饰植物种子细胞中产生的游离脂肪酸的比例的方法。本文例示的植物棕榈酰-ACP硫酯酶序列包括Cuphea，韭葱，芒果和榆树。还提供了作为这些棕榈酰-ACP硫酯酶序列表达的结果，在其种子中具有增加的C16：0脂肪酸水平的转基因植物。

6. 发明申请

WO1994010288A3 MEDIUM-CHAIN THIOESTERASES IN PLANTS 审中-公开
公开(公告)号：WO1994010288A3
公开(公告)日：1994-05-11
申请号：PCT/US1993010814
申请日：1993-10-29
申请人： CALGENE, INC. , VOELKER, Toni, Alois , DAVIES, Huw, Maelor , KNUTZON, Deborah, S.
发明人： CALGENE, INC.
IPC分类号： C12N15/55

7. 发明申请

WO1992020236A1 PLANT MEDIUM-CHAIN THIOESTERASES 审中-公开
标题翻译：植物中链链霉菌
公开(公告)号：WO1992020236A1
公开(公告)日：1992-11-26
申请号：PCT/US1992004332
申请日：1992-05-21
申请人： CALGENE, INC. , VOELKER, Toni, Alois , DAVIES, Huw, Maelor
发明人： CALGENE, INC.
IPC分类号： A23D07/00
CPC分类号： C07K14/415 , A23D9/00 , C12N9/16 , C12N15/8247 , C12P7/6418
摘要： By this invention, further properties and uses of plant medium-chain thioesterases are provided. In a first embodiment, this invention relates to plant seed and oil derived from that seed, which normally do not contain laurate, but now are found to contain laurate. In yet a different embodiment, this invention relates to a particular medium-chain thioesterase sequence, the Bay medium-chain thioesterase DNA sequence and to DNA constructs for the expression of this enzyme in a host cell. Other aspects of this invention relate to methods for using a plant medium-chain thioesterase. Expression of a plant medium-chain thioesterase in a bacterial cell to produce medium-chain fatty acids is provided. Methods to produce an unsaturated medium-chain thioesterase by the use of a plant medium-chain thioesterase are also described herein.
摘要翻译：通过本发明，提供了植物中链硫酯酶的进一步的性质和用途。在第一个实施方案中，本发明涉及来自该种子的植物种子和油，其通常不含月桂酸酯，但现在发现含有月桂酸酯。在另一个不同的实施方案中，本发明涉及特定的中链硫酯酶序列，Bay中链硫酯酶DNA序列和用于在宿主细胞中表达该酶的DNA构建体。本发明的其它方面涉及使用植物中链硫酯酶的方法。提供植物中链硫酯酶在细菌细胞中的表达以产生中链脂肪酸。本文还描述了通过使用植物中链硫酯酶生产不饱和中链硫酯酶的方法。

8. 发明申请

WO2009117417A3 CHIMERIC PROMOTERS AND THEIR USES THEREOF IN PLANTS 审中-公开
标题翻译： CHIMERIC PROMOTERS及其在植物中的用途
公开(公告)号：WO2009117417A3
公开(公告)日：2009-12-17
申请号：PCT/US2009037403
申请日：2009-03-17
申请人： MONSANTO TECHNOLOGY LLC , MROCZKA ANDREW M , RAVANELLO MONICA P , VOELKER TONI , WAGNER NICHOLAS W
发明人： MROCZKA ANDREW M , RAVANELLO MONICA P , VOELKER TONI , WAGNER NICHOLAS W
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8234
摘要： The present invention provides novel promoters for use in plants. Specifically, the present invention provides novel enhanced plant promoters. The present invention also provides DNA constructs; transgenic cells, plants, and seeds containing these novel promoters; and methods for preparing and using the same.
摘要翻译：本发明提供了用于植物中的新型启动子。具体地说，本发明提供了新型增强的植物启动子。本发明还提供了DNA构建体; 含有这些新型启动子的转基因细胞，植物和种子; 以及制备和使用它们的方法。

9. 发明申请

WO2007106728A3 SOYBEAN SEED AND OIL COMPOSITIONS AND METHODS OF MAKING SAME 审中-公开
标题翻译：大豆种子和油组合物及其制备方法
公开(公告)号：WO2007106728A3
公开(公告)日：2008-12-04
申请号：PCT/US2007063643
申请日：2007-03-09
申请人： MONSANTO TECHNOLOGY LLC , FILLATTI JOANNE J , KEITHLY GREG E , VOELKER TONI , ULMASOV TIM
发明人： FILLATTI JOANNE J , KEITHLY GREG E , VOELKER TONI , ULMASOV TIM
IPC分类号： A01H1/00 , A01H5/00 , C12N5/00 , C12N5/02 , C12N5/04 , C12N15/00 , C12N15/82 , C12N15/87 , C12P21/06
CPC分类号： C12N15/8247 , A01H5/10 , A23D9/00 , C11B1/10 , C11C1/002 , C12N15/8218
摘要： Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.
摘要翻译：公开了获得产生具有低亚麻酸水平和适度增加的油酸水平的种子的大豆植物的方法。还公开了用于生产具有低亚麻酸水平，适度增加的油酸水平和低饱和脂肪酸水平的种子的方法。这些方法需要提供中等油酸水平的转基因与大豆种质的组合，其含有赋予低亚麻酸表型的大豆基因中的突变。这些方法还需要提供中度油酸水平和低饱和脂肪水平的转基因与含有赋予低亚麻酸表型的大豆基因突变的大豆种质的组合。还公开了通过这些方法生产的大豆植物和种子。

10. 发明申请

WO2007095243A3 NUCLEIC ACID CONSTRUCTS AND METHODS FOR PRODUCING ALTERED SEED OIL COMPOSITIONS 审中-公开
标题翻译：核酸结构及其制备改良的种子油组合物的方法
公开(公告)号：WO2007095243A3
公开(公告)日：2007-12-21
申请号：PCT/US2007003823
申请日：2007-02-12
申请人： MONSANTO TECHNOLOGY LLC , VOELKER TONI , FILLATTI JOANNE J , BRINGE NEAL A , ULMASOV TIM
发明人： VOELKER TONI , FILLATTI JOANNE J , BRINGE NEAL A , ULMASOV TIM
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , A01H5/10 , C12N5/04 , C12N9/10 , C12P21/02
CPC分类号： C12N15/8247 , A01H5/10 , A23D9/00 , A23K10/30 , A23K20/147 , A23K20/158 , C10L1/026 , C11B1/00 , C12N9/0083 , C12N15/8216 , C12N15/8218 , C12N15/8237 , Y02E50/13
摘要： The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions, e.g. soybean plants bearing see, wherein the seed exhibit an oil composition which comprises 42-85% oleic acid and 1.5-8% saturated fatty acids. The genes to be suppressed are selected from the group consisting of FAD2, FAD3, and FATB, and the genes whose expression is to be enhanced are selected from the group consisting of beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, and delta-9 desaturase.
摘要翻译：本发明在植物遗传学领域，并且提供与脂肪酸合成途径中的多个基因的协调操作相关的重组核酸分子，构建体和其它试剂。特别地，本发明的试剂与脂肪酸合成途径中某些基因的同时增强表达相关联，并抑制同一途径中某些其他基因的表达。还提供了掺入这种试剂的植物，特别是掺入这种构建体的植物，其中植物表现出改变的种子油组合物，例如，观察大豆植物，其中种子显示包含42-85％油酸和1.5-8％饱和脂肪酸的油组合物。待抑制的基因选自FAD2，FAD3和FATB，其表达增强的基因选自β-酮酰基-ACP合酶I，β-酮酰基-ACP合成酶IV ，和Δ-9去饱和酶。

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