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2. 发明申请

WO1994010288A2 MEDIUM-CHAIN THIOESTERASES IN PLANTS 审中-公开
标题翻译：植物中的中等链球菌
公开(公告)号：WO1994010288A2
公开(公告)日：1994-05-11
申请号：PCT/US1993010814
申请日：1993-10-29
申请人： CALGENE, INC. , VOELKER, Toni, Alois , DAVIES, Huw, Maelor , KNUTZON, Deborah, S.
发明人： CALGENE, INC.
IPC分类号： C12N0
CPC分类号： A23D9/00 , C07K14/415 , C12N9/16 , C12N15/8247 , C12P7/6418
摘要： By this invention, further plant medium-chain acyl-ACP thioesterases are provided, as well as uses of long-chain thioesterase sequences in conjunction with medium-chain thioesterase sequences. In a first embodiment, this invention relates to particular medium-chain thioesterase sequences from elm and Cuphea, and to DNA constructs for the expression of these thioesterases in host cells for production of C8 and C10 fatty acids. Other aspects of this invention relate to methods for using plant medium-chain thioesterases or medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells. As a further aspect, uses of long-chain thioesterase sequences for anti-sense methods in plant cells in conjunction with expression of medium-chain thioesterases in plant cells is described.
摘要翻译：通过本发明，提供了另外的植物中链酰基-ACP硫酯酶，以及长链硫酯酶序列与中链硫酯酶序列的用途。在第一个实施方案中，本发明涉及来自elm和Cuphea的特定中链硫酯酶序列，以及用于在宿主细胞中表达这些硫酯酶以生产C8和C10脂肪酸的DNA构建体。本发明的其它方面涉及使用植物中链硫酯酶或来自非植物来源的中链硫酯酶在植物细胞中提供中链脂肪酸的方法。作为另一方面，描述了长链硫酯酶序列在植物细胞中用于反义方法与植物细胞中中链硫酯酶表达的用途。

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