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    • 3. 发明申请
    • OLIGONUCLEOTIDE SEQUENCE FORMULA FOR LABELING OLIGONUCLEOTIDE PROBE AND PROTEINS FOR IN-SITU ANALYSIS
    • 用于标记寡核苷酸探针和蛋白质的寡核苷酸序列配方用于现场分析
    • WO0222874A9
    • 2003-04-03
    • PCT/US0128014
    • 2001-09-06
    • VENTANA MED SYST INCUTERMOHLEN JOSEPH GCONNAUGHTON JOHN
    • UTERMOHLEN JOSEPH GCONNAUGHTON JOHN
    • G01N33/53C12N15/09C12Q1/68G01N33/532G01N33/569
    • C12Q1/6841C12Q1/6811C12Q1/6816C12Q1/6876C12Q1/6888C12Q2600/158C12Q2525/161C12Q2525/151C12Q2537/143
    • The present invention provides oligonucleotide probes and oligonucleotide probe collections and protein labeling for detecting or localizing a plurality nucleic acid target genes or antigens within a cell or tissue sample. Specifically, the provides collections of oligonucleotide probe for use in in situ hybridization analyses in which each probe has a label-domain with the sequence formulas of (CTATTTT)n, (AAAATAG)n or (TTTTATC)n or (GATAAAA)n in which all cases "n" would equal 1 or greater. The present invention provides collections or "cocktails" of oligonucleotide probes for detecting or localizing specific nucleic acid target genes within a cell or tissue sample. The cocktails are useful for detecting the following: the Kappa gene (SEQ ID NOS: 1-16 inclusive); the Lamba gene (SEQ ID NOS: 501-509, 511-513, and 515); the CMV (cytomegalovirus) gene (SEQ ID NOS: 221-241 inclusive); EBER (Epstein-Barr RNA) gene (SEQ IN NOS: 51-54 inclusive): Alu (SEQ IN NOS: 55-56); PolyA (SEQ ID NO: 57); and the detection tail (SEQ ID NO: 330).
    • 本发明提供了用于检测或定位细胞或组织样品内的多个核酸靶基因或抗原的寡核苷酸探针和寡核苷酸探针收集物和蛋白质标记。 具体地,提供用于原位杂交分析的寡核苷酸探针的集合,其中每个探针具有带有(CTATTTT)n,(AAAATAG)n或(TTTTATC)n或(GATAAAA)n的序列式的标记结构域,其中 所有情况“n”将等于1或更大。 本发明提供用于检测或定位细胞或组织样品中的特异性核酸靶基因的寡核苷酸探针的“鸡尾酒”。 鸡尾酒可用于检测以下内容:Kappa基因(SEQ ID NOS:1-16包含); Lamba基因(SEQ ID NO:501-509,511-513和515); CMV(巨细胞病毒)基因(SEQ ID NOS:221-241); EBER(Epstein-Barr RNA)基因(SEQ ID NO:51-54(含)):Alu(SEQ ID NO:55-56); PolyA(SEQ ID NO:57); 和检测尾(SEQ ID NO:330)。
    • 5. 发明申请
    • OLIGONUCLEOTIDES FOR LABELING OLIGONUCLEOTIDE PROBES AND PROTEINS
    • 用于标记寡核苷酸探针和蛋白质的寡核苷酸
    • WO0222874A3
    • 2003-09-25
    • PCT/US0128014
    • 2001-09-06
    • VENTANA MED SYST INCUTERMOHLEN JOSEPH GCONNAUGHTON JOHN
    • UTERMOHLEN JOSEPH GCONNAUGHTON JOHN
    • G01N33/53C12N15/09C12Q1/68G01N33/532G01N33/569
    • C12Q1/6841C12Q1/6811C12Q1/6816C12Q1/6876C12Q1/6888C12Q2600/158C12Q2525/161C12Q2525/151C12Q2537/143
    • The present invention provides oligonucleotide probes and oligonucleotide probe collections and protein labeling for detecting or localizing a plurality nucleic acid target genes or antigens within a cell or tissue sample. Specifically, the provides collections of oligonucleotide probe for use in in situ hybridization analyses in which each probe has a label-domain with the sequence formulas of (CTATTTT)n, (AAAATAG)n or (TTTTATC)n or (GATAAAA)n in which all cases "n" would equal 1 or greater. The present invention provides collections or "cocktails" of oligonucleotide probes for detecting or localizing specific nucleic acid target genes within a cell or tissue sample. The cocktails are useful for detecting the following: the Kappa gene (SEQ ID NOS: 1-16 inclusive); the Lamba gene (SEQ ID NOS: 501-509, 511-513, and 515); the CMV (cytomegalovirus) gene (SEQ ID NOS: 221-241 inclusive); EBER (Epstein-Barr RNA) gene (SEQ IN NOS: 51-54 inclusive): Alu (SEQ IN NOS: 55-56); PolyA (SEQ ID NO: 57); and the detection tail (SEQ ID NO: 330).
    • 本发明提供了用于检测或定位细胞或组织样品内的多个核酸靶基因或抗原的寡核苷酸探针和寡核苷酸探针收集物和蛋白质标记。 具体地,提供用于原位杂交分析的寡核苷酸探针的集合,其中每个探针具有带有(CTATTTT)n,(AAAATAG)n或(TTTTATC)n或(GATAAAA)n的序列式的标记结构域,其中 所有情况“n”将等于1或更大。 本发明提供用于检测或定位细胞或组织样品中的特异性核酸靶基因的寡核苷酸探针的“鸡尾酒”。 鸡尾酒可用于检测以下内容:Kappa基因(SEQ ID NOS:1-16包含); Lamba基因(SEQ ID NO:501-509,511-513和515); CMV(巨细胞病毒)基因(SEQ ID NO:221-241); EBER(Epstein-Barr RNA)基因(SEQ ID NO:51-54(含)):Alu(SEQ ID NO:55-56); PolyA(SEQ ID NO:57); 和检测尾(SEQ ID NO:330)。