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    • 10. 发明申请
    • METHOD OF OBTAINING GELATIN FROM AQUATIC ANIMALS
    • 从水生动物获得凝胶的方法
    • WO2014209101A2
    • 2014-12-31
    • PCT/MY2014/000191
    • 2014-06-25
    • UNIVERSITI PUTRA MALAYSIA
    • MD. YUSOFF, FatimahBAKAR, JamilahBASRI, MahiranISMAIL, MaznahKHONG, Mun Hoe Nicholas
    • C07K14/78
    • A61K35/614A61K38/00C07K14/461
    • The present invention relates to a simple, cost and time effective process of producing gelatin from aquatic animal tissue, which comprises the steps of: (a) treating the cleaned and dehydrated aquatic animal tissue with salt solution, preferably of less than 1.0M; (b) treating biomass obtained after Step (a) with aqueous alkali; (c) washing the alkali-treated biomass with water until the washing water is substantially neutral; (d) treating the neutralized biomass from step (c) with aqueous acid with continuous stirring for up to 4 h; (e) washing the acid-treated biomass with water until the washing water is substantially neutral; (f) extracting with water at pH less or equal to 2 at temperatures in the range of 45°C to 65°C for 6 to 8 h; (g) dialysing the gelatin obtained from step (f) against neutral buffer or deionised distilled water for at least 24 hours; (h) removal of water from the gelatin obtained until moisture content of the resultin gelatine is less than 13%. The process provides a gelatine having excellent properties, such as quality, appearance, gel strength etc., suitable for use in pharmaceutical, cosmeceutical, food and photography products.
    • 本发明涉及从水生动物组织制备明胶的简单,成本和时间有效的方法,其包括以下步骤:(a)用优选小于1.0M的盐溶液处理清洁和脱水的水生动物组织; (b)用碱水处理步骤(a)后获得的生物质; (c)用水洗涤碱处理的生物质,直到洗涤水基本上为中性; (d)用连续搅拌的酸水溶液处理来自步骤(c)的中和的生物质达4小时; (e)用水洗涤酸处理的生物质,直到洗涤水基本上为中性; (f)在45℃至65℃的温度下,用pH小于或等于2的水萃取6至8小时; (g)将从步骤(f)获得的明胶对中性缓冲液或去离子蒸馏水透析至少24小时; (h)从明胶中除去水,直到明胶的含水量低于13%。 该方法提供了具有优异性能的明胶,如质量,外观,凝胶强度等,适用于制药,药妆,食品和摄影产品。