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2. 发明申请

WO2007022543A2 METHODS AND KITS FOR DISTINGUISHING BETWEEN SPECIFIC AND NON-SPECIFIC PROTEIN ASSOCIATIONS 审中-公开
标题翻译：用于特异性和非特异性蛋白质结合之间鉴别的方法和工具
公开(公告)号：WO2007022543A2
公开(公告)日：2007-02-22
申请号：PCT/US2006032967
申请日：2006-08-21
申请人： UNIV ROCKEFELLER , CHAIT BRIAN T , TACKETT ALAN J , DEGRASSE JEFFREY A , OEFFINGER MARLENE , ROUT MICHAEL P , SEKEDAT MATTHEW D
发明人： CHAIT BRIAN T , TACKETT ALAN J , DEGRASSE JEFFREY A , OEFFINGER MARLENE , ROUT MICHAEL P , SEKEDAT MATTHEW D
IPC分类号： G01N33/567 , G01N33/53 , G01N33/554 , G01N33/569 , G01N33/574
CPC分类号： G01N33/5023 , G01N33/5032 , G01N33/60 , G01N33/6845 , G01N33/6848
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (H) proteins non-specifically associated with the tagged given protein, or (Hi) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
摘要翻译：本发明是确定细胞中给定蛋白质和其它蛋白质之间的缔合是否是特异性的方法。该方法包括（a）提供其中给定蛋白质含有标签的细胞的第一样品，（b）提供相同细胞的第二样品，其中所述给定蛋白质和其它蛋白质经代谢标记，并且其中（c）混合和裂解第一细胞样品和第二细胞样品以提供蛋白质的混合物，（d）将给定蛋白质的标签与底物结合，（e）分离蛋白质与标记的给定蛋白质结合至底物，其中相关蛋白质包含：（i）与标记的给定蛋白质特异性相关的蛋白质，（H）与标记的给定蛋白质非特异性相关的蛋白质，或（嗨）其组合，（f）确定每个相关蛋白是否未标记，或标记蛋白和未标记蛋白质的混合物，其中如果相关蛋白未被标记，那么该蛋白质特异性地与具有标记的蛋白质相关联给予蛋白质。

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